MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer4
MS data file : Ofer4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:07:42 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,499

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–17 (out of 17)


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+11

Accession Score Description
1 AMYG_ASPOR 106 Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2

+12

Accession Score Description
1 RNT2_ASPOR 102 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

+13

Accession Score Description
1 ABNC_ASPOR 99 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1

+14

Accession Score Description
1 Q2UTD2_ASPOR 82 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000064 PE=3 SV=1

-15

Accession Score Description
1 GFP_AEQVI 64 Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1
Score Mass Matches Sequences emPAI
15.1 GFP_AEQVI 64 26983 7 (2) 6 (2) 0.26
Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1
2 samesets of GFP_AEQVI
IGF1_BOVIN 64 42206 7 (2) 6 (2) 0.16
Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal
B7UCZ6_9RHAB 64 85329 7 (2) 6 (2) 0.08
G protein/GFP fusion protein OS=Recombinant vesicular stomatitis Indiana virus rVSV-G/GFP OX=582817 GN=G PE=3 SV=1

-7 peptide matches (7 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
4035   413.7126 825.4106 825.4055 6.25 0 10 0.26 +1Score > 24 indicates identity
Score > 16 indicates homology
U K.FICTTGK.L
4105   525.7679 1049.5212 1049.5142 6.74 0 13 0.36 +1Score > 27 indicates identity
Score > 21 indicates homology
U K.FEGDTLVNR.I
4144   641.7957 1281.5768 1281.5659 8.52 0 46 0.00052 +1Score > 26 indicates identity U K.SAMPEGYVQER.T + Oxidation (M)
4152   674.3365 1346.6584 1346.6507 5.78 1 13 0.11 +1Score > 27 indicates identity
Score > 15 indicates homology
U R.TIFFKDDGNYK.T
4153   449.8936 1346.6590 1346.6507 6.17 1 41 0.00046 +1Score > 27 indicates identity
Score > 20 indicates homology
U R.TIFFKDDGNYK.T
4166   493.2635 1476.7687 1476.7572 7.74 1 13 0.19 +1Score > 27 indicates identity
Score > 18 indicates homology
U R.AEVKFEGDTLVNR.I
4167   752.3389 1502.6632 1502.6525 7.13 0 15 0.53 +1Score > 25 indicates identity U K.FSVSGEGEGDATYGK.L

+16

Accession Score Description
1 LAP2_ASPOR 35 probable leucine aminopeptidase 2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lap2 PE=1 SV=1

+17

Accession Score Description
1 Q2U7H4_ASPOR 30 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000838 PE=3 SV=1
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