MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer4
MS data file : Ofer4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:07:42 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,499

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–17 (out of 17)


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+11

Accession Score Description
1 AMYG_ASPOR 106 Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2

+12

Accession Score Description
1 RNT2_ASPOR 102 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

-13

Accession Score Description
1 ABNC_ASPOR 99 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1
Score Mass Matches Sequences emPAI
13.1 ABNC_ASPOR 99 34396 8 (4) 5 (3) 0.45
Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1

-8 peptide matches (7 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3991 +1 331.1781 660.3416 660.3377 5.94 0 16 0.48 +1Score > 27 indicates identity
Score > 25 indicates homology
U R.INVCR.S
3994   340.6687 679.3228 679.3177 7.55 0 15 0.2 +1Score > 26 indicates identity
Score > 21 indicates homology
U R.GDFVDK.N
4111   360.5275 1078.5607 1078.5560 4.36 0 13 0.16 +1Score > 26 indicates identity
Score > 17 indicates homology
U K.NLWAPDVHK.G
4112   540.2882 1078.5618 1078.5560 5.45 0 34 0.01 +1Score > 26 indicates identity U K.NLWAPDVHK.G
4193   865.4056 1728.7966 1728.7856 6.39 0 16 0.038 +1Score > 26 indicates identity
Score > 15 indicates homology
U R.NGDLSVSSYQFGWNR.L
4195   865.8965 1729.7784 1729.7696 5.11 0 47 0.00026 +1Score > 25 indicates identity
Score > 23 indicates homology
U R.NGDLSVSSYQFGWNR.L + Deamidated (NQ)
4210   903.3908 1804.7670 1804.7540 7.23 0 63 1.3e-006 +1Score > 24 indicates identity
Score > 17 indicates homology
U K.ANGYDTSFPAQGEEYR.I + Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
Q2U9G9_ASPOR 16 61119 13.1
Amidase domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000035 PE=4 SV=1

+14

Accession Score Description
1 Q2UTD2_ASPOR 82 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000064 PE=3 SV=1

+15

Accession Score Description
1 GFP_AEQVI 64 Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1

+16

Accession Score Description
1 LAP2_ASPOR 35 probable leucine aminopeptidase 2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lap2 PE=1 SV=1

+17

Accession Score Description
1 Q2U7H4_ASPOR 30 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000838 PE=3 SV=1
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