MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer4
MS data file : Ofer4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:07:42 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,499

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–17 (out of 17)


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+11

Accession Score Description
1 AMYG_ASPOR 106 Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2

-12

Accession Score Description
1 RNT2_ASPOR 102 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2
Score Mass Matches Sequences emPAI
12.1 RNT2_ASPOR 102 31293 9 (5) 7 (4) 0.66
Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

-9 peptide matches (9 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3996   341.6766 681.3386 681.3333 7.78 0 14 0.63 +1Score > 24 indicates identity U K.GLDSYK.A
3997   682.3462 681.3389 681.3333 8.19 0 18 0.22 +1Score > 24 indicates identity U K.GLDSYK.A
4009   361.7103 721.4060 721.4010 6.93 0 36 0.0035 +1Score > 24 indicates identity U K.TVDLFK.G
4010   722.4138 721.4065 721.4010 7.59 0 27 0.025 +1Score > 24 indicates identity U K.TVDLFK.G
4054   437.2409 872.4672 872.4603 7.92 0 48 0.0003 +1Score > 28 indicates identity
Score > 25 indicates homology
U K.AGIVPDSSK.T
4073   476.2459 950.4772 950.4709 6.68 0 50 0.00028 +1Score > 27 indicates identity U K.EVGDYLQK.T
4088   500.7585 999.5024 999.4947 7.79 0 35 0.0012 +1Score > 26 indicates identity
Score > 18 indicates homology
U R.TELLSYMK.K + Oxidation (M)
4184   560.9278 1679.7616 1679.7283 19.8 0 18 0.34 +1Score > 26 indicates identity U K.HGTCINTIEPSCYK.D + Deamidated (NQ)
4295   856.7549 2567.2429 2567.2061 14.3 0 8 0.2 +1Score > 27 indicates identity
Score > 14 indicates homology
U K.GNAITGEYQPIDTLTSPGCSTSGIK.Y + Deamidated (NQ)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
Q2UDP7_ASPOR 27 201276 12.1
AAA domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090012000087 PE=4 SV=1

+13

Accession Score Description
1 ABNC_ASPOR 99 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1

+14

Accession Score Description
1 Q2UTD2_ASPOR 82 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000064 PE=3 SV=1

+15

Accession Score Description
1 GFP_AEQVI 64 Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1

+16

Accession Score Description
1 LAP2_ASPOR 35 probable leucine aminopeptidase 2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lap2 PE=1 SV=1

+17

Accession Score Description
1 Q2U7H4_ASPOR 30 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000838 PE=3 SV=1
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