MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer4
MS data file : Ofer4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:07:42 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,499

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–17 (out of 17)


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-11

Accession Score Description
1 AMYG_ASPOR 106 Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2
Score Mass Matches Sequences emPAI
11.1 AMYG_ASPOR 106 65929 7 (5) 6 (5) 0.28
Glucoamylase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=glaA PE=2 SV=2

-7 peptide matches (7 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
4033   412.2225 822.4304 822.4236 8.35 0 34 0.0077 +1Score > 26 indicates identity
Score > 25 indicates homology
U K.VVTDSFR.S
4080   490.7656 979.5166 979.5087 8.15 0 31 0.0099 +1Score > 25 indicates identity
Score > 24 indicates homology
U R.SIYAINSGR.A
4082   491.2511 980.4876 980.4927 -5.13 0 13 1.2 +3Score > 26 indicates identity U R.SIYAINSGR.A + Deamidated (NQ)
4090   504.7754 1007.5362 1007.5287 7.44 0 2 1 +1Score > 26 indicates identity
Score > 14 indicates homology
U R.ALVEGSSFAK.T
4091   507.7747 1013.5348 1013.5254 9.33 0 53 8.6e-005 +1Score > 26 indicates identity
Score > 25 indicates homology
U R.AENQAVAVGR.Y
4103   524.2686 1046.5226 1046.5145 7.78 0 41 0.0026 +1Score > 27 indicates identity U K.SAVQSDVWR.-
4108   534.7869 1067.5592 1067.5499 8.77 0 46 0.00033 +1Score > 25 indicates identity
Score > 23 indicates homology
U K.ATTVYGESIK.I

+12

Accession Score Description
1 RNT2_ASPOR 102 Ribonuclease T2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rntB PE=1 SV=2

+13

Accession Score Description
1 ABNC_ASPOR 99 Probable arabinan endo-1,5-alpha-L-arabinosidase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=abnC PE=3 SV=1

+14

Accession Score Description
1 Q2UTD2_ASPOR 82 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000064 PE=3 SV=1

+15

Accession Score Description
1 GFP_AEQVI 64 Green fluorescent protein OS=Aequorea victoria GN=GFP PE=1 SV=1

+16

Accession Score Description
1 LAP2_ASPOR 35 probable leucine aminopeptidase 2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lap2 PE=1 SV=1

+17

Accession Score Description
1 Q2U7H4_ASPOR 30 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000838 PE=3 SV=1
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