MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer4
MS data file : Ofer4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:07:42 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,499

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 17)

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+1

Accession Score Description
1 AMYA1_ASPOR 586 Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1

+2

Accession Score Description
1 LAPA_ASPOR 289 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+3

Accession Score Description
1 P20D2_ASPOR 190 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 189 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2U8Y3_ASPOR 186 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1

+6

Accession Score Description
1 Q2UB64_ASPOR 180 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+7

Accession Score Description
1 Q2ULW8_ASPOR 174 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1

+8

Accession Score Description
1 EGLC_ASPOR 161 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+9

Accession Score Description
1 TRYP_PIG 138 Trypsin OS=Sus scrofa PE=1 SV=1

-10

Accession Score Description
1 Q2U2Z5_ASPOR 107 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1
Score Mass Matches Sequences emPAI
10.1 Q2U2Z5_ASPOR 107 14669 9 (7) 5 (4) 3.31
Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1

-9 peptide matches (9 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
4117   369.8792 1106.6158 1106.6084 6.65 0 4 0.79 +1Score > 25 indicates identity
Score > 15 indicates homology		 U R.SVVVHLPDNK.R
4118   554.3154 1106.6162 1106.6084 7.08 0 12 0.97 +1Score > 24 indicates identity U R.SVVVHLPDNK.R
4140   316.6867 1262.7177 1262.7095 6.48 1 26 0.029 +1Score > 24 indicates identity U R.SVVVHLPDNKR.I
4141   632.3668 1262.7190 1262.7095 7.55 1 29 0.0024 +1Score > 24 indicates identity
Score > 15 indicates homology		 U R.SVVVHLPDNKR.I
4142   421.9139 1262.7199 1262.7095 8.20 1 29 0.017 +1Score > 24 indicates identity U R.SVVVHLPDNKR.I
4183   556.5799 1666.7179 1666.7046 7.98 0 26 0.0077 +1Score > 24 indicates identity
Score > 17 indicates homology		 U K.DGNCYATGGHLDPYK.R
4191   573.6006 1717.7800 1717.7730 4.07 0 56 7.6e-006 +1Score > 26 indicates identity
Score > 18 indicates homology		 U R.INCGNFHLVSDGEEK.K
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer4.raw

Score > 26 indicates identity
4215   608.6141 1822.8205 1822.8057 8.12 1 38 0.0037 -1Score > 26 indicates identity U K.DGNCYATGGHLDPYKR.G
-5.31 1 11 1.7 2 NMRQMVLELNASDDR   + 2 Oxidation (M)
12.9 0 7 4.8 3 LYDSTDSDHTGTAGLDR  
19.1 0 4 8.7 4 TGELDANGPQSVADYER   + 2 Deamidated (NQ)
2.69 0 3 10 5 ATQATVDALGDMSHAYR   + Oxidation (M); Deamidated (NQ)
-46.9 1 2 14 6 LKPQSALQNSSGYSGKR   + 3 Deamidated (NQ)
-39.4 0 1 15 7 QMSLLYPGLEETWTR  
-28.9 0 1 15 7 TASSVTSSAPICVASTQR   + Deamidated (NQ)
12.4 1 0 21 9 RMEEWMADDAEAVVR   + Oxidation (M)
4216   456.7129 1822.8225 1822.8057 9.23 1 25 0.013 +1Score > 26 indicates identity
Score > 19 indicates homology		 U K.DGNCYATGGHLDPYKR.G
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