MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Ofer4
MS data file : Ofer4.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 7 May 2026 at 18:07:42 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,499

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Protein families 1–10 (out of 17)

Page: 1 2 Next 

-1

Accession Score Description
1 AMYA1_ASPOR 586 Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1
Score Mass Matches Sequences emPAI
1.1 AMYA1_ASPOR 586 55288 25 (18) 16 (14) 1.67
Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1
1 sameset of AMYA1_ASPOR
AMYA3_ASPOR 586 55283 25 (18) 16 (14) 1.67
Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1

-25 peptide matches (24 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3956   575.3442 574.3369 574.3326 7.48 0 29 0.031 +1Score > 27 indicates identity U R.IDTVK.H
4001   348.1924 694.3702 694.3650 7.62 0 37 0.0022 +1Score > 23 indicates identity U R.NYAISK.D
4002   695.3777 694.3704 694.3650 7.87 0 16 0.25 +1Score > 23 indicates identity U R.NYAISK.D
4031   410.7245 819.4344 819.4279 7.99 0 19 0.13 +1Score > 27 indicates identity
Score > 22 indicates homology		 U K.NWPIYK.D
4044   849.4763 848.4690 848.4644 5.50 0 12 1 +1Score > 25 indicates identity
Score > 25 indicates homology		 U R.VLYPTEK.L
4045   425.2429 848.4712 848.4644 8.12 0 35 0.0029 +1Score > 25 indicates identity
Score > 22 indicates homology		 U R.VLYPTEK.L
4065   464.7858 927.5570 927.5501 7.46 0 54 5.9e-005 +1Score > 24 indicates identity U K.LIASANAIR.N
4066   930.4618 929.4545 929.4495 5.45 0 22 0.027 +1Score > 26 indicates identity
Score > 19 indicates homology		 U K.DTGFVTYK.N
4067   465.7350 929.4554 929.4495 6.44 0 18 0.14 +1Score > 26 indicates identity
Score > 22 indicates homology		 U K.DTGFVTYK.N
4083   328.5160 982.5262 982.5196 6.73 0 46 0.00038 +1Score > 24 indicates identity U K.ALSSALHER.G
4084   492.2711 982.5276 982.5196 8.23 0 76 4.3e-007 +1Score > 24 indicates identity U K.ALSSALHER.G
4096   1026.4723 1025.4650 1025.4607 4.24 0 17 0.062 +1Score > 24 indicates identity
Score > 18 indicates homology		 U K.DFWPGYNK.A
4097   513.7412 1025.4678 1025.4607 6.99 0 20 0.074 +1Score > 25 indicates identity
Score > 21 indicates homology		 U K.DFWPGYNK.A
4147   657.3444 1312.6742 1312.6663 6.06 0 95 2.8e-009 +1Score > 26 indicates identity
Score > 22 indicates homology		 U R.FASYTNDIALAK.N
4148 +1 657.8372 1313.6598 1313.6503 7.27 0 102 1.6e-009 +1Score > 27 indicates identity U R.FASYTNDIALAK.N + Deamidated (NQ)
4151   671.8588 1341.7030 1341.6929 7.60 0 62 1.1e-005 +1Score > 27 indicates identity
Score > 25 indicates homology		 U R.SQSIYFLLTDR.F
4161   716.8904 1431.7662 1431.7569 6.51 0 86 5.5e-008 +1Score > 26 indicates identity U K.GTDGSQIVTILSNK.G
4170   520.9610 1559.8612 1559.8519 5.96 1 46 0.00016 +1Score > 25 indicates identity
Score > 21 indicates homology		 U R.KGTDGSQIVTILSNK.G
4171   780.9380 1559.8614 1559.8519 6.13 1 30 0.0042 +1Score > 25 indicates identity
Score > 19 indicates homology		 U R.KGTDGSQIVTILSNK.G
4196   580.6234 1738.8484 1738.8348 7.78 1 41 0.00064 +1Score > 27 indicates identity
Score > 21 indicates homology		 U K.NWPIYKDDTTIAMR.K + Oxidation (M)
4221   930.4434 1858.8722 1858.8625 5.25 0 35 0.001 +1Score > 27 indicates identity
Score > 18 indicates homology		 U R.EATWLSGYPTDSELYK.L
4230   954.4273 1906.8400 1906.8288 5.88 0 72 1.2e-006 +1Score > 25 indicates identity U K.STSGSMDDLYNMINTVK.S + 2 Oxidation (M)
4231   636.9562 1907.8468 1907.8129 17.8 0 22 0.097 +1Score > 25 indicates identity U K.STSGSMDDLYNMINTVK.S + 2 Oxidation (M); Deamidated (NQ)
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer4.raw

Score > 25 indicates identity

Score > 20 indicates homology
4263   759.0130 2274.0172 2273.9859 13.8 0 57 1.4e-005 -1Score > 25 indicates identity
Score > 20 indicates homology		 U K.SDCPDSTLLGTFVENHDNPR.F + Deamidated (NQ)
13.8 0 29 0.0077 2 K.SDCPDSTLLGTFVENHDNPR.F + Deamidated (NQ)
-8.96 1 5 2.1 3 NFTQGMREGFTDIFTQTHK   + Oxidation (M); Deamidated (NQ)
-24.6 1 5 2.1 4 ANIDAVGSDNRTPLICAAAMGR   + 2 Deamidated (NQ)

+2

Accession Score Description
1 LAPA_ASPOR 289 Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1

+3

Accession Score Description
1 P20D2_ASPOR 190 Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1

+4

Accession Score Description
1 ORYZ_ASPOR 189 Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2

+5

Accession Score Description
1 Q2U8Y3_ASPOR 186 Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1

+6

Accession Score Description
1 Q2UB64_ASPOR 180 SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1

+7

Accession Score Description
1 Q2ULW8_ASPOR 174 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1

+8

Accession Score Description
1 EGLC_ASPOR 161 Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1

+9

Accession Score Description
1 TRYP_PIG 138 Trypsin OS=Sus scrofa PE=1 SV=1

+10

Accession Score Description
1 Q2U2Z5_ASPOR 107 Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1
Page: 1 2 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/