MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Ofer4 |
| MS data file | : | Ofer4.mgf |
| Database | : | A-oryzae 20191108 (12,205 sequences; 5,465,702 residues) |
| Timestamp | : | 7 May 2026 at 18:07:42 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,499 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 23 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMYA1_ASPOR | 586 | Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 1.1 | AMYA1_ASPOR |
586 | 55288 | 25 (18) | 16 (14) | 1.67 | |
| Alpha-amylase A type-1/2 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy1 PE=1 SV=1 | |||||||
| 1 sameset of AMYA1_ASPOR | |||||||
| AMYA3_ASPOR |
586 | 55283 | 25 (18) | 16 (14) | 1.67 | ||
| Alpha-amylase A type-3 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=amy3 PE=3 SV=1 | |||||||
25 peptide matches (24 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 3956 |
575.3442 | 574.3369 | 574.3326 | 7.48 | 0 | 29 | 0.031 | 1Score > 27 indicates identity |
U | R.IDTVK.H | |
| 4001 |
348.1924 | 694.3702 | 694.3650 | 7.62 | 0 | 37 | 0.0022 | 1Score > 23 indicates identity |
U | R.NYAISK.D | |
| 4002 |
695.3777 | 694.3704 | 694.3650 | 7.87 | 0 | 16 | 0.25 | 1Score > 23 indicates identity |
U | R.NYAISK.D | |
D:\Xcalibur\Data\Jennifer\PRT1426 Ofer gel\Ofer4.rawScore > 27 indicates identity Score > 22 indicates homology |
|||||||||||
| 4031 |
410.7245 | 819.4344 | 819.4279 | 7.99 | 0 | 19 | 0.13 | 1Score > 27 indicates identity Score > 22 indicates homology |
U | K.NWPIYK.D | |
| 26.6 | 0 | 9 | 1.2 | 2 | PNADIYK | ||||||
| 22.5 | 1 | 8 | 1.5 | 3 | AVEKDMK | ||||||
| 22.5 | 0 | 8 | 1.5 | 3 | GLEQVMK + Oxidation (M) | ||||||
| -21.9 | 1 | 8 | 1.5 | 3 | LGEKVMK + Oxidation (M) | ||||||
| -22.0 | 0 | 8 | 1.5 | 3 | VVTQMVK + Oxidation (M) | ||||||
| 43.6 | 0 | 6 | 2.2 | 7 | NAPHQPR + Deamidated (NQ) | ||||||
| 48.3 | 0 | 5 | 2.7 | 8 | YHMELK | ||||||
| -17.8 | 1 | 5 | 3.1 | 9 | DPKGLYK | ||||||
| 4044 |
849.4763 | 848.4690 | 848.4644 | 5.50 | 0 | 12 | 1 | 1Score > 25 indicates identity Score > 25 indicates homology |
U | R.VLYPTEK.L | |
| 4045 |
425.2429 | 848.4712 | 848.4644 | 8.12 | 0 | 35 | 0.0029 | 1Score > 25 indicates identity Score > 22 indicates homology |
U | R.VLYPTEK.L | |
| 4065 |
464.7858 | 927.5570 | 927.5501 | 7.46 | 0 | 54 | 5.9e-005 | 1Score > 24 indicates identity |
U | K.LIASANAIR.N | |
| 4066 |
930.4618 | 929.4545 | 929.4495 | 5.45 | 0 | 22 | 0.027 | 1Score > 26 indicates identity Score > 19 indicates homology |
U | K.DTGFVTYK.N | |
| 4067 |
465.7350 | 929.4554 | 929.4495 | 6.44 | 0 | 18 | 0.14 | 1Score > 26 indicates identity Score > 22 indicates homology |
U | K.DTGFVTYK.N | |
| 4083 |
328.5160 | 982.5262 | 982.5196 | 6.73 | 0 | 46 | 0.00038 | 1Score > 24 indicates identity |
U | K.ALSSALHER.G | |
| 4084 |
492.2711 | 982.5276 | 982.5196 | 8.23 | 0 | 76 | 4.3e-007 | 1Score > 24 indicates identity |
U | K.ALSSALHER.G | |
| 4096 |
1026.4723 | 1025.4650 | 1025.4607 | 4.24 | 0 | 17 | 0.062 | 1Score > 24 indicates identity Score > 18 indicates homology |
U | K.DFWPGYNK.A | |
| 4097 |
513.7412 | 1025.4678 | 1025.4607 | 6.99 | 0 | 20 | 0.074 | 1Score > 25 indicates identity Score > 21 indicates homology |
U | K.DFWPGYNK.A | |
| 4147 |
657.3444 | 1312.6742 | 1312.6663 | 6.06 | 0 | 95 | 2.8e-009 | 1Score > 26 indicates identity Score > 22 indicates homology |
U | R.FASYTNDIALAK.N | |
| 4148 |
1 |
657.8372 | 1313.6598 | 1313.6503 | 7.27 | 0 | 102 | 1.6e-009 | 1Score > 27 indicates identity |
U | R.FASYTNDIALAK.N + Deamidated (NQ) |
| 4151 |
671.8588 | 1341.7030 | 1341.6929 | 7.60 | 0 | 62 | 1.1e-005 | 1Score > 27 indicates identity Score > 25 indicates homology |
U | R.SQSIYFLLTDR.F | |
| 4161 |
716.8904 | 1431.7662 | 1431.7569 | 6.51 | 0 | 86 | 5.5e-008 | 1Score > 26 indicates identity |
U | K.GTDGSQIVTILSNK.G | |
| 4170 |
520.9610 | 1559.8612 | 1559.8519 | 5.96 | 1 | 46 | 0.00016 | 1Score > 25 indicates identity Score > 21 indicates homology |
U | R.KGTDGSQIVTILSNK.G | |
| 4171 |
780.9380 | 1559.8614 | 1559.8519 | 6.13 | 1 | 30 | 0.0042 | 1Score > 25 indicates identity Score > 19 indicates homology |
U | R.KGTDGSQIVTILSNK.G | |
| 4196 |
580.6234 | 1738.8484 | 1738.8348 | 7.78 | 1 | 41 | 0.00064 | 1Score > 27 indicates identity Score > 21 indicates homology |
U | K.NWPIYKDDTTIAMR.K + Oxidation (M) | |
| 4221 |
930.4434 | 1858.8722 | 1858.8625 | 5.25 | 0 | 35 | 0.001 | 1Score > 27 indicates identity Score > 18 indicates homology |
U | R.EATWLSGYPTDSELYK.L | |
| 4230 |
954.4273 | 1906.8400 | 1906.8288 | 5.88 | 0 | 72 | 1.2e-006 | 1Score > 25 indicates identity |
U | K.STSGSMDDLYNMINTVK.S + 2 Oxidation (M) | |
| 4231 |
636.9562 | 1907.8468 | 1907.8129 | 17.8 | 0 | 22 | 0.097 | 1Score > 25 indicates identity |
U | K.STSGSMDDLYNMINTVK.S + 2 Oxidation (M); Deamidated (NQ) | |
| 4263 |
759.0130 | 2274.0172 | 2273.9859 | 13.8 | 0 | 57 | 1.4e-005 | 1Score > 25 indicates identity Score > 20 indicates homology |
U | K.SDCPDSTLLGTFVENHDNPR.F + Deamidated (NQ) | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LAPA_ASPOR | 289 | Leucine aminopeptidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=lapA PE=1 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | P20D2_ASPOR | 190 | Probable carboxypeptidase AO090003000058 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000058 PE=3 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ORYZ_ASPOR | 189 | Alkaline protease 1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alp1 PE=1 SV=2 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U8Y3_ASPOR | 186 | Chitin-binding type-4 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000246 PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2UB64_ASPOR | 180 | SGNH_hydro domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090102000092 PE=4 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2ULW8_ASPOR | 174 | Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000239 PE=4 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EGLC_ASPOR | 161 | Probable glucan endo-1,3-beta-glucosidase eglC OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglC PE=3 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 138 | Trypsin OS=Sus scrofa PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q2U2Z5_ASPOR | 107 | Sod_Cu domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000258 PE=4 SV=1 |
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