MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1202 481.7188 961.4230 961.4253 -2.38 0 0 1.6 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
2075 549.2496 1096.4846 1096.4893 -4.20 0 0 1.7 +1Score > 15 indicates identity DEMIMSAIR + 2 Oxidation (M)
2785 616.7802 1231.5458 1231.5390 5.53 0 0 1.7 +1Score > 15 indicates identity ADNDMAPLQQK + 2 Deamidated (NQ)
5575 846.3846 1690.7546 1690.7403 8.48 0 0 2 +1Score > 16 indicates identity DGQTAMSIHVMQGER + 2 Oxidation (M)
3136 643.7925 1285.5704 1285.5609 7.45 0 0 1.6 +1Score > 15 indicates identity SSDLFSICSDR
5259 819.3723 1636.7300 1636.7119 11.1 1 0 2.4 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
2336 576.2618 1150.5090 1150.5254 -14.2 1 0 1.8 +1Score > 15 indicates identity LEFNNDNRK + 2 Deamidated (NQ)
5971 873.3969 1744.7792 1744.7735 3.28 0 0 3.4 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
2834 616.7802 1231.5458 1231.5615 -12.7 1 0 1.7 +1Score > 15 indicates identity IREMSDNDPR
3966 711.3232 1420.6318 1420.6252 4.68 1 0 2.4 +1Score > 16 indicates identity ANNAINDMVRQR + 4 Deamidated (NQ); Oxidation (M)
102 387.1801 772.3456 772.3351 13.6 0 0 0.98 +1Score > 13 indicates identity DPNGDVR + Deamidated (NQ)
2188 562.7557 1123.4968 1123.4968 0.046 0 0 1.5 +1Score > 14 indicates identity DAFIDEMQR
4280 738.3355 1474.6564 1474.6657 -6.25 1 0 1.9 +1Score > 15 indicates identity ELHMGRMSQPTR + Deamidated (NQ); 2 Oxidation (M)
1704 522.2372 1042.4598 1042.4576 2.17 0 0 0.99 +1Score > 13 indicates identity GFADAIMMR + 2 Oxidation (M)
4765 778.8539 1555.6932 1555.7163 -14.8 0 0 1.9 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
1680 522.2372 1042.4598 1042.4542 5.42 0 0 0.99 +1Score > 13 indicates identity MNFNNVFR + 2 Deamidated (NQ)
2185 562.7557 1123.4968 1123.4968 0.046 0 0 1.5 +1Score > 14 indicates identity DAFIDEMQR
3157 643.7925 1285.5704 1285.5761 -4.41 0 0 1.6 +1Score > 15 indicates identity MQAYFDQLDR
3461 670.8048 1339.5950 1339.5900 3.74 0 0 2.4 +1Score > 16 indicates identity MMLEHYQQTK + 2 Oxidation (M)
4500 751.8416 1501.6686 1501.6507 11.9 0 0 2.7 +1Score > 17 indicates identity EASGFGADYNAMIR + Deamidated (NQ)
5122 805.8662 1609.7178 1609.7150 1.80 1 0 1.8 +1Score > 15 indicates identity MKLLMPDNMSQER + 2 Deamidated (NQ); Oxidation (M)
5152 805.8662 1609.7178 1609.7150 1.80 1 0 1.8 +1Score > 15 indicates identity MKLLMPDNMSQER + 2 Deamidated (NQ); Oxidation (M)
6454 913.9153 1825.8160 1825.8251 -4.96 0 0 2.8 +1Score > 17 indicates identity MTNNDTTLQLSSVLNR + 4 Deamidated (NQ); Oxidation (M)
1 387.1682 772.3218
2 387.1759 772.3372
3 387.1759 772.3372
4 387.1759 772.3372
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78 387.1759 772.3372
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