MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 701–800 (out of 7204)

Page: Previous 1 …3 4 5 6 7 8 9 10 11 12 13 … 73 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4042 711.3232 1420.6318 1420.6512 -13.6 1 0 2.2 +1Score > 16 indicates identity QMMGMEPKQIAR + 2 Deamidated (NQ)
4479 751.8416 1501.6686 1501.6909 -14.8 1 0 2.4 +1Score > 17 indicates identity QNRSGAEHFGLQR + 3 Deamidated (NQ)
5312 819.3723 1636.7300 1636.7403 -6.24 0 0 2.2 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
6452 913.9153 1825.8160 1825.8451 -15.9 1 0 2.5 +1Score > 17 indicates identity ASGGFAMLAVDQREAMR + Deamidated (NQ); Oxidation (M)
548 427.6943 853.3740 853.3600 16.5 0 0 0.89 +1Score > 13 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
892 454.7066 907.3986 907.3957 3.29 0 0 1.2 +1Score > 14 indicates identity QMQNELK + 2 Deamidated (NQ); Oxidation (M)
6557 927.4214 1852.8282 1852.8241 2.25 1 0 2.3 +1Score > 17 indicates identity EWYEKHGNSHQGEPR
3470 670.8048 1339.5950 1339.6125 -13.0 1 0 2.1 +1Score > 16 indicates identity AANQAFMMERR + Oxidation (M)
665 441.1931 880.3716 880.3562 17.5 0 0 1.1 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
3632 684.3109 1366.6072 1366.6266 -14.1 1 0 2 +1Score > 16 indicates identity DWRETNIDYR
6318 900.4101 1798.8056 1798.8229 -9.61 1 0 1.8 +1Score > 15 indicates identity ALTFCEAEMDNLTRK + Deamidated (NQ)
2079 549.2496 1096.4846 1096.4715 12.0 0 0 1.5 +1Score > 15 indicates identity NMGGMASLMGK + Deamidated (NQ)
2190 562.7557 1123.4968 1123.5067 -8.75 0 0 1.4 +1Score > 14 indicates identity QQETAVATMK + 2 Deamidated (NQ); Oxidation (M)
3173 643.7925 1285.5704 1285.5609 7.45 0 0 1.5 +1Score > 15 indicates identity SSDLFSICSDR
6231 900.4092 1798.8038 1798.8270 -12.9 0 0 1.6 +1Score > 15 indicates identity VINGFMIQGGGFEPGMK + 2 Deamidated (NQ); Oxidation (M)
6749 940.9276 1879.8406 1879.8469 -3.35 0 0 2.7 +1Score > 17 indicates identity NEVVADGNDSVTMTATVR + 2 Deamidated (NQ)
4786 778.8539 1555.6932 1555.7154 -14.3 0 0 1.7 +1Score > 15 indicates identity ALQQYTADQFNAGK + 2 Deamidated (NQ)
5107 805.8662 1609.7178 1609.7406 -14.1 0 0 1.6 +1Score > 15 indicates identity MLQESVDALFDNGR + Oxidation (M)
1820 535.7434 1069.4722 1069.4862 -13.1 0 0 0.91 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
2159 562.7557 1123.4968 1123.5154 -16.5 0 0 1.4 +1Score > 14 indicates identity IMGWMEGLR + 2 Oxidation (M)
3147 643.7925 1285.5704 1285.5761 -4.41 0 0 1.5 +1Score > 15 indicates identity MQAYFDQLDR
4267 738.3355 1474.6564 1474.6776 -14.3 1 0 1.8 +1Score > 15 indicates identity NAWWVGSKDCPR
5268 819.3723 1636.7300 1636.7119 11.1 1 0 2.2 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
5291 819.3723 1636.7300 1636.7403 -6.24 0 0 2.2 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
2514 589.7679 1177.5212 1177.5332 -10.1 1 0 1.3 +1Score > 14 indicates identity ANNPTSRMMR + Deamidated (NQ)
2675 603.2741 1204.5336 1204.5506 -14.1 0 0 2 +1Score > 16 indicates identity GCGITVDQAER
3180 643.7925 1285.5704 1285.5609 7.46 0 0 1.5 +1Score > 15 indicates identity DNGAEYTMTLR + Oxidation (M)
6405 913.9153 1825.8160 1825.8482 -17.6 1 0 2.5 +1Score > 17 indicates identity EDAQLLYGFNNKQER + 2 Deamidated (NQ)
6730 940.9276 1879.8406 1879.8557 -7.98 0 0 2.7 +1Score > 17 indicates identity SCAFLIADGVMPSNENR
3814 697.8171 1393.6196 1393.6217 -1.50 0 0 2.2 +1Score > 16 indicates identity SMQDPIADMLTR + Deamidated (NQ); Oxidation (M)
4968 792.3600 1582.7054 1582.6875 11.4 0 0 1.5 +1Score > 15 indicates identity VYFCDPQSPWQR + Deamidated (NQ)
5786 859.8907 1717.7668 1717.8006 -19.6 1 0 1.7 +1Score > 15 indicates identity SQILDEAKAEAEQER + 2 Deamidated (NQ)
4456 751.8416 1501.6686 1501.6727 -2.71 1 0 2.5 +1Score > 17 indicates identity RMPAMMNYTAGVK + Deamidated (NQ); 2 Oxidation (M)
5509 846.3795 1690.7444 1690.7475 -1.79 0 0 1.5 +1Score > 15 indicates identity ESSVPFSYYDNQQK
2789 616.7802 1231.5458 1231.5615 -12.7 1 0 1.6 +1Score > 15 indicates identity IREMSDNDPR
3175 643.7925 1285.5704 1285.5761 -4.41 0 0 1.5 +1Score > 15 indicates identity MQAYFDQLDR
3385 657.2986 1312.5826 1312.5686 10.7 1 0 2 +1Score > 16 indicates identity IMDRMTMNGGR + 2 Oxidation (M)
4440 751.8416 1501.6686 1501.6891 -13.6 1 0 2.5 +1Score > 17 indicates identity WEWSRYYAWR
133 387.1807 772.3468 772.3351 15.2 0 0 0.92 +1Score > 13 indicates identity DPNGDVR + Deamidated (NQ)
1197 481.7188 961.4230 961.4253 -2.38 0 0 1.5 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
3357 657.2986 1312.5826 1312.5686 10.7 1 0 2 +1Score > 16 indicates identity IMDRMTMNGGR + 2 Oxidation (M)
3613 684.3109 1366.6072 1366.6266 -14.1 1 0 2 +1Score > 16 indicates identity DWRETNIDYR
3928 711.3232 1420.6318 1420.6252 4.68 1 0 2.2 +1Score > 16 indicates identity ANNAINDMVRQR + 4 Deamidated (NQ); Oxidation (M)
6113 886.9030 1771.7914 1771.7909 0.29 0 0 2.1 +1Score > 16 indicates identity EIVFMGTPEFAAECR + Oxidation (M)
1584 508.7311 1015.4476 1015.4570 -9.24 0 0 1.1 +1Score > 13 indicates identity TADEQQAPR + Deamidated (NQ)
6565 927.4214 1852.8282 1852.8625 -18.5 1 0 2.4 +1Score > 17 indicates identity LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
3246 657.2906 1312.5666 1312.5686 -1.49 1 0 1.4 +1Score > 14 indicates identity IMDRMTMNGGR + 2 Oxidation (M)
6410 913.9153 1825.8160 1825.8186 -1.39 1 0 2.6 +1Score > 17 indicates identity ERQTGMQMVQSVLER + 3 Deamidated (NQ); 2 Oxidation (M)
3216 643.7925 1285.5704 1285.5649 4.31 0 0 1.5 +1Score > 15 indicates identity DGDVMNFLFNV + Oxidation (M)
784 441.2004 880.3862 880.3927 -7.31 0 0 1.5 +1Score > 15 indicates identity DGDGFGVSK
5897 873.3969 1744.7792 1744.7839 -2.64 1 0 3.3 +1Score > 18 indicates identity GGQYCSADYQAQLKR + Deamidated (NQ)
6246 900.4092 1798.8038 1798.7870 9.35 1 0 1.6 +1Score > 15 indicates identity QPNGQVWSPQNDDRR + 3 Deamidated (NQ)
3481 670.8048 1339.5950 1339.6112 -12.0 0 0 2.2 +1Score > 16 indicates identity ASVSMQMGIQVR + 2 Deamidated (NQ); 2 Oxidation (M)
2996 630.2854 1258.5562 1258.5621 -4.62 0 0 0.98 +1Score > 13 indicates identity ISMMPGMPSHR + Oxidation (M)
5579 846.3846 1690.7546 1690.7832 -16.9 0 0 2 +1Score > 16 indicates identity NGIANNMLLENGGSLR + 3 Deamidated (NQ); Oxidation (M)
287 400.6820 799.3494 799.3501 -0.76 0 0 0.94 +1Score > 13 indicates identity YPDYSR
3201 643.7925 1285.5704 1285.5761 -4.41 0 0 1.5 +1Score > 15 indicates identity MQAYFDQLDR
601 427.6943 853.3740 853.3600 16.5 0 0 0.94 +1Score > 13 indicates identity MTSSAGQR + Deamidated (NQ); Oxidation (M)
3494 670.8048 1339.5950 1339.5900 3.74 0 0 2.3 +1Score > 16 indicates identity MMLEHYQQTK + 2 Oxidation (M)
3806 697.8171 1393.6196 1393.6329 -9.53 1 0 2.3 +1Score > 16 indicates identity EEMATEMERLR
5224 819.3723 1636.7300 1636.7457 -9.53 0 0 2.3 +1Score > 16 indicates identity VWWAETTWQMQR + Oxidation (M)
5304 819.3723 1636.7300 1636.7371 -4.31 1 0 2.3 +1Score > 16 indicates identity CVELVREMMGEQR + Deamidated (NQ)
2642 603.2741 1204.5336 1204.5394 -4.75 0 0 2.1 +1Score > 16 indicates identity ADCEQLAELR + Deamidated (NQ)
1839 535.7434 1069.4722 1069.4862 -13.1 1 0 0.94 +1Score > 13 indicates identity EAMGFSEKR + Oxidation (M)
2136 562.7557 1123.4968 1123.5179 -18.8 0 0 1.5 +1Score > 14 indicates identity SASLSDIEMR + Oxidation (M)
5903 873.3969 1744.7792 1744.7735 3.28 0 0 3.3 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
6725 940.9276 1879.8406 1879.8305 5.41 1 0 2.8 +1Score > 17 indicates identity QSHGNPLMKQGHQMQR + 4 Deamidated (NQ)
4266 738.3355 1474.6564 1474.6324 16.3 0 0 1.8 +1Score > 15 indicates identity QHQQFADQESVR + 3 Deamidated (NQ)
4471 751.8416 1501.6686 1501.6806 -7.95 1 0 2.6 +1Score > 17 indicates identity RLDFMMQEFNR + Oxidation (M)
5944 873.3969 1744.7792 1744.7838 -2.63 1 0 3.3 +1Score > 18 indicates identity AREASGFGADYNAMIR + Deamidated (NQ); Oxidation (M)
2179 562.7557 1123.4968 1123.4894 6.65 0 0 1.5 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
2374 576.2618 1150.5090 1150.5117 -2.32 0 0 1.8 +1Score > 15 indicates identity YMTWDNPPK
4954 792.3600 1582.7054 1582.7045 0.58 1 0 1.6 +1Score > 15 indicates identity CQYETLVENNRR + 2 Deamidated (NQ)
2904 616.7802 1231.5458 1231.5431 2.26 0 0 1.7 +1Score > 15 indicates identity TNFNFAIMEK + 2 Deamidated (NQ); Oxidation (M)
6280 900.4092 1798.8038 1798.8196 -8.76 0 0 1.7 +1Score > 15 indicates identity TEGATVFGYQVMDPER
580 427.6943 853.3740 853.3851 -13.0 0 0 0.95 +1Score > 13 indicates identity GEMSTVSK + Oxidation (M)
2475 589.7679 1177.5212 1177.5107 8.95 0 0 1.4 +1Score > 14 indicates identity MQQSIDPAMR + 2 Deamidated (NQ)
6141 886.9030 1771.7914 1771.7909 0.29 0 0 2.1 +1Score > 16 indicates identity EIVFMGTPEFAAECR + Oxidation (M)
3391 657.2986 1312.5826 1312.5686 10.7 1 0 2.1 +1Score > 16 indicates identity IMDRMTMNGGR + 2 Oxidation (M)
3674 684.3109 1366.6072 1366.6299 -16.6 1 0 2.1 +1Score > 16 indicates identity SRDAFIDEMQR
4466 751.8416 1501.6686 1501.6909 -14.8 1 0 2.6 +1Score > 17 indicates identity QNRSGAEHFGLQR + 3 Deamidated (NQ)
5914 873.3969 1744.7792 1744.7735 3.28 0 0 3.4 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
6871 954.4337 1906.8528 1906.8877 -18.3 1 0 2.8 +1Score > 17 indicates identity EQQLEVMTGCRTGQIR + 2 Deamidated (NQ)
762 441.2004 880.3862 880.3926 -7.26 0 0 1.5 +1Score > 15 indicates identity QQGEVYR + 2 Deamidated (NQ)
1086 468.2127 934.4108 934.4178 -7.46 1 0 1.8 +1Score > 15 indicates identity LDEDCKR
1831 535.7434 1069.4722 1069.4862 -13.1 0 0 0.96 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
5074 805.8662 1609.7178 1609.7253 -4.66 0 0 1.7 +1Score > 15 indicates identity LSNVSQSQNDLMTR + 2 Deamidated (NQ); Oxidation (M)
5983 873.3971 1744.7796 1744.7947 -8.61 0 0 3.4 +1Score > 18 indicates identity MIGPNCPGVITPGECK + Oxidation (M)
2487 589.7679 1177.5212 1177.5363 -12.8 1 0 1.4 +1Score > 14 indicates identity EGENPYANKR + Deamidated (NQ)
3144 643.7925 1285.5704 1285.5609 7.46 0 0 1.6 +1Score > 15 indicates identity DNGAEYTMTLR + Oxidation (M)
6272 900.4092 1798.8038 1798.8308 -15.0 0 0 1.7 +1Score > 15 indicates identity IYDSDSILSMHHEPR
6437 913.9153 1825.8160 1825.7900 14.2 0 0 2.7 +1Score > 17 indicates identity NGYNSAALNGDMNQALR + 2 Deamidated (NQ); Oxidation (M)
3341 657.2986 1312.5826 1312.6081 -19.4 1 0 2.1 +1Score > 16 indicates identity AGGTIMFSNNKR + 2 Deamidated (NQ); Oxidation (M)
6573 927.4214 1852.8282 1852.8625 -18.5 1 0 2.5 +1Score > 17 indicates identity LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
2505 589.7679 1177.5212 1177.5332 -10.1 1 0 1.4 +1Score > 14 indicates identity ANNPTSRMMR + Deamidated (NQ)
6772 940.9276 1879.8406 1879.8469 -3.35 0 0 2.9 +1Score > 17 indicates identity NEVVADGNDSVTMTATVR + 2 Deamidated (NQ)
1070 468.2127 934.4108 934.4178 -7.46 1 0 1.8 +1Score > 15 indicates identity LDEDCKR
6242 900.4092 1798.8038 1798.8308 -15.0 0 0 1.7 +1Score > 15 indicates identity IYDSDSILSMHHEPR
3930 711.3232 1420.6318 1420.6252 4.68 1 0 2.4 +1Score > 16 indicates identity ANNAINDMVRQR + 4 Deamidated (NQ); Oxidation (M)
4140 724.8293 1447.6440 1447.6725 -19.7 1 0 1.2 +1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
Page: Previous 1 …3 4 5 6 7 8 9 10 11 12 13 … 73 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/