MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 7001–7100 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
7077 967.9399 1933.8652
7078 967.9399 1933.8652
7079 967.9399 1933.8652
7080 967.9399 1933.8652
7081 967.9399 1933.8652
7083 967.9399 1933.8652
7084 967.9399 1933.8652
7085 967.9399 1933.8652
7086 967.9399 1933.8652
7087 967.9399 1933.8652
7088 967.9399 1933.8652
7089 967.9399 1933.8652
7090 967.9399 1933.8652
7091 967.9399 1933.8652
7092 967.9399 1933.8652
7093 967.9399 1933.8652
7094 967.9399 1933.8652
7095 967.9399 1933.8652
7096 967.9399 1933.8652
7097 967.9399 1933.8652
7098 967.9399 1933.8652
7099 967.9399 1933.8652
7100 967.9399 1933.8652
7103 967.9399 1933.8652
7104 967.9399 1933.8652
7105 967.9399 1933.8652
7106 967.9399 1933.8652
7108 967.9399 1933.8652
7111 967.9399 1933.8652
7112 967.9399 1933.8652
7113 967.9399 1933.8652
7114 967.9399 1933.8652
7115 967.9399 1933.8652
7117 967.9399 1933.8652
7118 967.9399 1933.8652
7119 967.9399 1933.8652
7120 967.9399 1933.8652
7121 967.9399 1933.8652
7122 967.9399 1933.8652
7123 967.9399 1933.8652
7124 967.9399 1933.8652
7125 967.9399 1933.8652
7126 967.9399 1933.8652
7127 980.1954 1958.3762
7128 980.1954 1958.3762
7129 980.1954 1958.3762
7130 980.1954 1958.3762
7131 980.1954 1958.3762
7132 980.1954 1958.3762
7133 980.1954 1958.3762
7134 980.1954 1958.3762
7135 980.1954 1958.3762
7136 980.1954 1958.3762
7137 980.1954 1958.3762
7138 980.1954 1958.3762
7139 980.1954 1958.3762
7140 980.1954 1958.3762
7141 980.1954 1958.3762
7142 980.1954 1958.3762
7143 980.1954 1958.3762
7144 980.1954 1958.3762
7145 980.1954 1958.3762
7146 980.1954 1958.3762
7147 980.1954 1958.3762
7148 980.1954 1958.3762
7149 980.1954 1958.3762
7150 980.1954 1958.3762
7151 980.1954 1958.3762
7152 980.1954 1958.3762
7153 980.1954 1958.3762
7154 980.1954 1958.3762
7155 980.1954 1958.3762
7156 980.1954 1958.3762
7157 980.1954 1958.3762
7158 980.1954 1958.3762
7159 980.1954 1958.3762
7160 980.1954 1958.3762
7161 980.1954 1958.3762
7162 980.1954 1958.3762
7163 980.1954 1958.3762
7164 980.1954 1958.3762
7165 980.1954 1958.3762
7166 980.1954 1958.3762
7167 980.1954 1958.3762
7168 980.1954 1958.3762
7169 980.1954 1958.3762
7170 980.1954 1958.3762
7171 980.1954 1958.3762
7172 980.1954 1958.3762
7173 980.1954 1958.3762
7174 980.1954 1958.3762
7175 980.1954 1958.3762
7176 980.1954 1958.3762
7177 980.1954 1958.3762
7178 980.1954 1958.3762
7179 980.1954 1958.3762
7180 980.1954 1958.3762
7181 980.1954 1958.3762
7182 980.1954 1958.3762
7183 980.1954 1958.3762
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