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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6901–7000 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6966 967.9255 1933.8364
6967 967.9399 1933.8652
6968 967.9399 1933.8652
6969 967.9399 1933.8652
6970 967.9399 1933.8652
6971 967.9399 1933.8652
6972 967.9399 1933.8652
6973 967.9399 1933.8652
6974 967.9399 1933.8652
6975 967.9399 1933.8652
6976 967.9399 1933.8652
6977 967.9399 1933.8652
6978 967.9399 1933.8652
6980 967.9399 1933.8652
6981 967.9399 1933.8652
6982 967.9399 1933.8652
6983 967.9399 1933.8652
6984 967.9399 1933.8652
6985 967.9399 1933.8652
6986 967.9399 1933.8652
6988 967.9399 1933.8652
6989 967.9399 1933.8652
6990 967.9399 1933.8652
6991 967.9399 1933.8652
6992 967.9399 1933.8652
6993 967.9399 1933.8652
6995 967.9399 1933.8652
6996 967.9399 1933.8652
6997 967.9399 1933.8652
6998 967.9399 1933.8652
6999 967.9399 1933.8652
7000 967.9399 1933.8652
7001 967.9399 1933.8652
7002 967.9399 1933.8652
7003 967.9399 1933.8652
7005 967.9399 1933.8652
7006 967.9399 1933.8652
7007 967.9399 1933.8652
7008 967.9399 1933.8652
7009 967.9399 1933.8652
7010 967.9399 1933.8652
7011 967.9399 1933.8652
7012 967.9399 1933.8652
7013 967.9399 1933.8652
7014 967.9399 1933.8652
7015 967.9399 1933.8652
7016 967.9399 1933.8652
7017 967.9399 1933.8652
7018 967.9399 1933.8652
7019 967.9399 1933.8652
7020 967.9399 1933.8652
7022 967.9399 1933.8652
7023 967.9399 1933.8652
7024 967.9399 1933.8652
7025 967.9399 1933.8652
7026 967.9399 1933.8652
7027 967.9399 1933.8652
7029 967.9399 1933.8652
7030 967.9399 1933.8652
7031 967.9399 1933.8652
7032 967.9399 1933.8652
7033 967.9399 1933.8652
7034 967.9399 1933.8652
7035 967.9399 1933.8652
7036 967.9399 1933.8652
7037 967.9399 1933.8652
7038 967.9399 1933.8652
7039 967.9399 1933.8652
7040 967.9399 1933.8652
7041 967.9399 1933.8652
7042 967.9399 1933.8652
7043 967.9399 1933.8652
7044 967.9399 1933.8652
7045 967.9399 1933.8652
7047 967.9399 1933.8652
7048 967.9399 1933.8652
7049 967.9399 1933.8652
7050 967.9399 1933.8652
7051 967.9399 1933.8652
7052 967.9399 1933.8652
7054 967.9399 1933.8652
7055 967.9399 1933.8652
7056 967.9399 1933.8652
7058 967.9399 1933.8652
7059 967.9399 1933.8652
7060 967.9399 1933.8652
7061 967.9399 1933.8652
7062 967.9399 1933.8652
7063 967.9399 1933.8652
7064 967.9399 1933.8652
7065 967.9399 1933.8652
7066 967.9399 1933.8652
7068 967.9399 1933.8652
7069 967.9399 1933.8652
7070 967.9399 1933.8652
7071 967.9399 1933.8652
7072 967.9399 1933.8652
7073 967.9399 1933.8652
7074 967.9399 1933.8652
7075 967.9399 1933.8652
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