MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5989 873.4040 1744.7934 1744.7904 1.75 0 1 3.3 +1Score > 19 indicates identity DIDVDYASHSPQIER + Deamidated (NQ)
6251 900.4092 1798.8038 1798.8308 -15.0 0 1 1.4 +1Score > 15 indicates identity IYDSDSILSMHHEPR
4040 711.3232 1420.6318 1420.6187 9.25 1 1 2 +1Score > 16 indicates identity MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M)
5409 832.8785 1663.7424 1663.7406 1.10 1 1 1.7 +1Score > 16 indicates identity MDPSLNTMANDRQR + Oxidation (M)
608 427.6943 853.3740 853.3786 -5.34 0 1 0.8 +1Score > 13 indicates identity NGTMMIR + 2 Oxidation (M)
6082 886.9030 1771.7914 1771.7584 18.7 1 1 1.8 +1Score > 16 indicates identity DPASDQMQHWKEQR + Deamidated (NQ); Oxidation (M)
691 441.1942 880.3738 880.3562 20.0 0 1 1 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
4293 738.3355 1474.6564 1474.6663 -6.70 0 1 1.6 +1Score > 15 indicates identity SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M)
6891 954.4337 1906.8528 1906.8652 -6.47 0 1 2.3 +1Score > 17 indicates identity ATIDGLENMNSPEMVAAK + Deamidated (NQ); Oxidation (M)
2200 562.7557 1123.4968 1123.4894 6.65 0 1 1.2 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
4448 751.8416 1501.6686 1501.6658 1.90 1 1 2.2 +1Score > 17 indicates identity AEHRGGSGNFAEDR
4806 778.8539 1555.6932 1555.7123 -12.2 1 1 1.5 +1Score > 15 indicates identity FVENKSQLMGACR + Deamidated (NQ); Oxidation (M)
2149 562.7557 1123.4968 1123.5067 -8.75 0 1 1.3 +1Score > 14 indicates identity QQETAVATMK + 2 Deamidated (NQ); Oxidation (M)
4450 751.8416 1501.6686 1501.6658 1.90 1 1 2.2 +1Score > 17 indicates identity AEHRGGSGNFAEDR
86 387.1788 772.3430 772.3351 10.2 0 1 0.81 +1Score > 13 indicates identity DPNGDVR + Deamidated (NQ)
1827 535.7434 1069.4722 1069.4862 -13.1 0 1 0.81 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
1902 535.7434 1069.4722 1069.4902 -16.8 0 1 0.81 +1Score > 13 indicates identity YQLNMWSK + Deamidated (NQ)
2527 589.7679 1177.5212 1177.5363 -12.8 1 1 1.2 +1Score > 14 indicates identity EGENPYANKR + Deamidated (NQ)
3125 643.7925 1285.5704 1285.5761 -4.41 0 1 1.3 +1Score > 15 indicates identity MQAYFDQLDR
6383 913.9153 1825.8160 1825.8451 -15.9 1 1 2.3 +1Score > 17 indicates identity ASGGFAMLAVDQREAMR + Deamidated (NQ); Oxidation (M)
6994 967.9399 1933.8652 1933.8705 -2.70 1 1 2.2 +1Score > 17 indicates identity IMCAFKMMATCSNVVR + Oxidation (M)
671 441.1934 880.3722 880.3562 18.2 0 1 1 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
770 441.2004 880.3862 880.3926 -7.26 0 1 1.3 +1Score > 15 indicates identity QQGEVYR + 2 Deamidated (NQ)
651 441.1925 880.3704 880.3862 -17.8 0 1 1.1 +1Score > 14 indicates identity GFCAGVDR
4141 724.8293 1447.6440 1447.6579 -9.59 0 1 1 +1Score > 13 indicates identity TLWVSDDAEEQR
3343 657.2986 1312.5826 1312.5571 19.4 0 1 1.8 +1Score > 16 indicates identity YGDAFTQPEQR + 2 Deamidated (NQ)
4352 738.3355 1474.6564 1474.6582 -1.22 1 1 1.6 +1Score > 15 indicates identity AECQNQQGNLRR + 2 Deamidated (NQ)
6538 927.4214 1852.8282 1852.8241 2.25 1 1 2.1 +1Score > 17 indicates identity EWYEKHGNSHQGEPR
1187 481.7188 961.4230 961.4253 -2.38 0 1 1.3 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
3159 643.7925 1285.5704 1285.5761 -4.41 0 1 1.4 +1Score > 15 indicates identity MQAYFDQLDR
2368 576.2618 1150.5090 1150.4891 17.4 0 1 1.5 +1Score > 15 indicates identity DEAQEQQFR + Deamidated (NQ)
5420 832.8785 1663.7424 1663.7408 0.97 0 1 1.7 +1Score > 16 indicates identity GMSVEMLFDFMAVR + 2 Oxidation (M)
6738 940.9276 1879.8406 1879.8258 7.90 0 1 2.5 +1Score > 17 indicates identity MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M)
6903 954.4337 1906.8528 1906.8690 -8.48 1 1 2.4 +1Score > 17 indicates identity LSAEDNARVNVAMNQTR + 3 Deamidated (NQ); Oxidation (M)
3310 657.2986 1312.5826 1312.5686 10.7 1 1 1.8 +1Score > 16 indicates identity IMDRMTMNGGR + 2 Oxidation (M)
5275 819.3723 1636.7300 1636.7119 11.1 1 1 2 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
2143 562.7557 1123.4968 1123.4894 6.65 0 1 1.3 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
2488 589.7679 1177.5212 1177.5107 8.95 0 1 1.2 +1Score > 14 indicates identity MQQSIDPAMR + 2 Deamidated (NQ)
4612 765.3478 1528.6810 1528.6715 6.24 0 1 1.3 +1Score > 15 indicates identity EMEQTAFSAELTR + Deamidated (NQ); Oxidation (M)
6859 954.4337 1906.8528 1906.8877 -18.3 1 1 2.4 +1Score > 17 indicates identity EQQLEVMTGCRTGQIR + 2 Deamidated (NQ)
3311 657.2986 1312.5826 1312.5757 5.26 0 1 1.8 +1Score > 16 indicates identity EYTWANLMER + Deamidated (NQ)
6919 954.4337 1906.8528 1906.8877 -18.3 1 1 2.4 +1Score > 17 indicates identity EQQLEVMTGCRTGQIR + 2 Deamidated (NQ)
4513 751.8416 1501.6686 1501.6909 -14.8 1 1 2.3 +1Score > 17 indicates identity QNRSGAEHFGLQR + 3 Deamidated (NQ)
5273 819.3723 1636.7300 1636.7119 11.1 1 1 2.1 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
5313 819.3723 1636.7300 1636.7403 -6.24 0 1 2.1 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
6563 927.4214 1852.8282 1852.8631 -18.8 0 1 2.1 +1Score > 17 indicates identity SSIQLISSNWPYWNR + 3 Deamidated (NQ)
3766 697.8171 1393.6196 1393.6118 5.61 0 1 2 +1Score > 16 indicates identity MMLDNHALYDR + Oxidation (M)
5477 832.8785 1663.7424 1663.7487 -3.73 1 1 1.8 +1Score > 16 indicates identity ASETFMYARAMGWK + Oxidation (M)
6445 913.9153 1825.8160 1825.8451 -15.9 1 1 2.4 +1Score > 17 indicates identity ASGGFAMLAVDQREAMR + Deamidated (NQ); Oxidation (M)
5303 819.3723 1636.7300 1636.7403 -6.24 0 1 2.1 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
3505 670.8048 1339.5950 1339.6143 -14.4 0 1 2 +1Score > 16 indicates identity TGVSNTLENEFK + 2 Deamidated (NQ)
3122 643.7925 1285.5704 1285.5761 -4.41 0 1 1.4 +1Score > 15 indicates identity MQAYFDQLDR
5552 846.3846 1690.7546 1690.7403 8.49 0 1 1.8 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
5109 805.8662 1609.7178 1609.7161 1.07 0 1 1.5 +1Score > 15 indicates identity GEAVWFAPDQDYGR
5992 873.4066 1744.7986 1744.8110 -7.09 1 1 3.7 +1Score > 19 indicates identity FFRFNTAWQWGER + Deamidated (NQ)
685 441.1940 880.3734 880.3562 19.5 0 1 1.1 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
3142 643.7925 1285.5704 1285.5761 -4.41 0 1 1.4 +1Score > 15 indicates identity MQAYFDQLDR
5746 859.8907 1717.7668 1717.7577 5.32 0 1 1.6 +1Score > 15 indicates identity GAQLASDDNMLPDSQR + Deamidated (NQ)
5933 873.3969 1744.7792 1744.8057 -15.1 1 1 3 +1Score > 18 indicates identity QGDFKSPEGFYSVQR + Deamidated (NQ)
3492 670.8048 1339.5950 1339.6125 -13.0 1 1 2.1 +1Score > 16 indicates identity AANQAFMMERR + Oxidation (M)
1194 481.7188 961.4230 961.4253 -2.38 0 1 1.4 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
3124 643.7925 1285.5704 1285.5761 -4.41 0 1 1.4 +1Score > 15 indicates identity MQAYFDQLDR
3489 670.8048 1339.5950 1339.6125 -13.0 1 1 2.1 +1Score > 16 indicates identity AANQAFMMERR + Oxidation (M)
4472 751.8416 1501.6686 1501.6685 0.097 0 1 2.3 +1Score > 17 indicates identity TDTGSQQGYFLAGR + 2 Deamidated (NQ)
4449 751.8416 1501.6686 1501.6806 -7.95 1 1 2.3 +1Score > 17 indicates identity RLDFMMQEFNR + Oxidation (M)
6396 913.9153 1825.8160 1825.7900 14.2 0 1 2.4 +1Score > 17 indicates identity NGYNSAALNGDMNQALR + 2 Deamidated (NQ); Oxidation (M)
6597 927.4214 1852.8282 1852.8340 -3.10 1 1 2.2 +1Score > 17 indicates identity TVNGETAERWQYDER
3791 697.8171 1393.6196 1393.6078 8.49 1 1 2.1 +1Score > 16 indicates identity RNGMSGVQCVER + 2 Deamidated (NQ)
1198 481.7188 961.4230 961.4141 9.31 0 1 1.4 +1Score > 15 indicates identity NGWENNVK + 2 Deamidated (NQ)
3339 657.2986 1312.5826 1312.5830 -0.24 0 1 1.9 +1Score > 16 indicates identity NHCEILQGNAR + 2 Deamidated (NQ)
4787 778.8539 1555.6932 1555.7154 -14.3 0 1 1.6 +1Score > 15 indicates identity ALQQYTADQFNAGK + 2 Deamidated (NQ)
1176 481.7188 961.4230 961.4063 17.5 0 1 1.4 +1Score > 15 indicates identity METPQPDK + Deamidated (NQ); Oxidation (M)
2065 549.2496 1096.4846 1096.4893 -4.20 0 1 1.5 +1Score > 15 indicates identity DEMIMSAIR + 2 Oxidation (M)
6451 913.9153 1825.8160 1825.8451 -15.9 1 1 2.4 +1Score > 17 indicates identity ASGGFAMLAVDQREAMR + Deamidated (NQ); Oxidation (M)
6726 940.9276 1879.8406 1879.8291 6.13 0 1 2.6 +1Score > 17 indicates identity MIQEQTMLNVADNSGAR + 3 Deamidated (NQ)
890 454.7066 907.3986 907.3817 18.6 1 1 1.2 +1Score > 14 indicates identity ENMGRER + Deamidated (NQ); Oxidation (M)
3148 643.7925 1285.5704 1285.5761 -4.41 0 1 1.4 +1Score > 15 indicates identity MQAYFDQLDR
3327 657.2986 1312.5826 1312.5571 19.4 0 1 1.9 +1Score > 16 indicates identity YGDAFTQPEQR + 2 Deamidated (NQ)
3474 670.8048 1339.5950 1339.5748 15.1 0 1 2.1 +1Score > 16 indicates identity AMQDQVMELSR + Deamidated (NQ); 2 Oxidation (M)
6924 954.4337 1906.8528 1906.8883 -18.6 0 1 2.5 +1Score > 17 indicates identity MNTYSITLPWPPSNNR + Deamidated (NQ); Oxidation (M)
2337 576.2618 1150.5090 1150.5117 -2.32 0 1 1.6 +1Score > 15 indicates identity YMTWDNPPK
4950 792.3600 1582.7054 1582.6967 5.56 1 1 1.4 +1Score > 15 indicates identity ASMDELRQAAESMK + Deamidated (NQ); Oxidation (M)
5324 819.3723 1636.7300 1636.7403 -6.24 0 1 2.1 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
569 427.6943 853.3740 853.3851 -13.0 1 1 0.88 +1Score > 13 indicates identity MSSKEQK + Deamidated (NQ); Oxidation (M)
6294 900.4092 1798.8038 1798.8196 -8.76 0 1 1.5 +1Score > 15 indicates identity TEGATVFGYQVMDPER
6588 927.4214 1852.8282 1852.8625 -18.5 1 1 2.2 +1Score > 17 indicates identity LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
4318 738.3355 1474.6564 1474.6663 -6.70 0 1 1.7 +1Score > 15 indicates identity SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M)
6888 954.4337 1906.8528 1906.8784 -13.4 0 1 2.6 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
6695 940.9276 1879.8406 1879.8630 -11.9 0 1 2.6 +1Score > 17 indicates identity MTAQLACDALQMALWR + 2 Deamidated (NQ)
6894 954.4337 1906.8528 1906.8877 -18.3 1 1 2.6 +1Score > 17 indicates identity EQQLEVMTGCRTGQIR + 2 Deamidated (NQ)
1840 535.7434 1069.4722 1069.4862 -13.1 0 1 0.89 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
5116 805.8662 1609.7178 1609.7150 1.80 1 1 1.6 +1Score > 15 indicates identity MKLLMPDNMSQER + 2 Deamidated (NQ); Oxidation (M)
5280 819.3723 1636.7300 1636.7185 7.05 0 1 2.2 +1Score > 16 indicates identity VGGLSVMCEDAEAAGR + Oxidation (M)
6039 886.9030 1771.7914 1771.8154 -13.5 1 1 2 +1Score > 16 indicates identity VMALVGENGAGKSTMMK + Deamidated (NQ); 3 Oxidation (M)
6087 886.9030 1771.7914 1771.7947 -1.86 1 1 2 +1Score > 16 indicates identity ENCIFLDNPHQARR + 3 Deamidated (NQ)
808 441.2085 880.4024 880.4113 -10.0 0 1 2.5 +1Score > 17 indicates identity YMELGPR + Oxidation (M)
2679 603.2741 1204.5336 1204.5394 -4.75 0 1 2 +1Score > 16 indicates identity ADCEQLAELR + Deamidated (NQ)
1867 535.7434 1069.4722 1069.4902 -16.8 0 1 0.89 +1Score > 13 indicates identity YQLNMWSK + Deamidated (NQ)
2344 576.2618 1150.5090 1150.5117 -2.32 0 1 1.6 +1Score > 15 indicates identity YMTWDNPPK
6072 886.9030 1771.7914 1771.7584 18.7 1 1 2 +1Score > 16 indicates identity DPASDQMQHWKEQR + Deamidated (NQ); Oxidation (M)
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