MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6801–6900 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6850 954.4337 1906.8528
6851 954.4337 1906.8528
6852 954.4337 1906.8528
6853 954.4337 1906.8528
6854 954.4337 1906.8528
6855 954.4337 1906.8528
6856 954.4337 1906.8528
6858 954.4337 1906.8528
6860 954.4337 1906.8528
6861 954.4337 1906.8528
6862 954.4337 1906.8528
6863 954.4337 1906.8528
6864 954.4337 1906.8528
6865 954.4337 1906.8528
6866 954.4337 1906.8528
6867 954.4337 1906.8528
6868 954.4337 1906.8528
6869 954.4337 1906.8528
6872 954.4337 1906.8528
6873 954.4337 1906.8528
6874 954.4337 1906.8528
6875 954.4337 1906.8528
6876 954.4337 1906.8528
6877 954.4337 1906.8528
6878 954.4337 1906.8528
6879 954.4337 1906.8528
6881 954.4337 1906.8528
6882 954.4337 1906.8528
6884 954.4337 1906.8528
6885 954.4337 1906.8528
6886 954.4337 1906.8528
6887 954.4337 1906.8528
6889 954.4337 1906.8528
6890 954.4337 1906.8528
6893 954.4337 1906.8528
6895 954.4337 1906.8528
6896 954.4337 1906.8528
6897 954.4337 1906.8528
6898 954.4337 1906.8528
6900 954.4337 1906.8528
6901 954.4337 1906.8528
6902 954.4337 1906.8528
6905 954.4337 1906.8528
6906 954.4337 1906.8528
6907 954.4337 1906.8528
6908 954.4337 1906.8528
6909 954.4337 1906.8528
6910 954.4337 1906.8528
6911 954.4337 1906.8528
6912 954.4337 1906.8528
6913 954.4337 1906.8528
6914 954.4337 1906.8528
6916 954.4337 1906.8528
6917 954.4337 1906.8528
6918 954.4337 1906.8528
6920 954.4337 1906.8528
6921 954.4337 1906.8528
6922 954.4337 1906.8528
6923 954.4337 1906.8528
6925 954.4337 1906.8528
6926 954.4337 1906.8528
6927 954.4337 1906.8528
6928 954.4337 1906.8528
6929 954.4337 1906.8528
6930 954.4337 1906.8528
6931 954.4337 1906.8528
6932 954.4337 1906.8528
6933 954.4337 1906.8528
6934 954.4337 1906.8528
6935 954.4337 1906.8528
6936 954.4337 1906.8528
6937 954.4337 1906.8528
6938 954.4337 1906.8528
6939 954.4337 1906.8528
6940 954.4337 1906.8528
6941 954.4337 1906.8528
6942 954.4337 1906.8528
6943 954.4337 1906.8528
6944 954.4337 1906.8528
6945 954.4337 1906.8528
6946 954.4337 1906.8528
6947 954.4337 1906.8528
6948 954.4337 1906.8528
6949 954.4337 1906.8528
6950 954.4337 1906.8528
6951 954.4337 1906.8528
6952 954.4337 1906.8528
6953 954.4337 1906.8528
6954 954.4337 1906.8528
6955 954.4337 1906.8528
6956 954.4337 1906.8528
6957 954.4337 1906.8528
6958 954.4337 1906.8528
6959 954.4349 1906.8552
6960 954.4379 1906.8612
6961 954.4381 1906.8616
6962 954.4387 1906.8628
6963 954.4399 1906.8652
6964 954.4405 1906.8664
6965 954.4438 1906.8730
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