MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6701–6800 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6733 940.9276 1879.8406
6734 940.9276 1879.8406
6735 940.9276 1879.8406
6736 940.9276 1879.8406
6739 940.9276 1879.8406
6740 940.9276 1879.8406
6741 940.9276 1879.8406
6742 940.9276 1879.8406
6743 940.9276 1879.8406
6744 940.9276 1879.8406
6745 940.9276 1879.8406
6747 940.9276 1879.8406
6748 940.9276 1879.8406
6751 940.9276 1879.8406
6752 940.9276 1879.8406
6753 940.9276 1879.8406
6754 940.9276 1879.8406
6755 940.9276 1879.8406
6756 940.9276 1879.8406
6757 940.9276 1879.8406
6758 940.9276 1879.8406
6759 940.9276 1879.8406
6760 940.9276 1879.8406
6761 940.9276 1879.8406
6762 940.9276 1879.8406
6763 940.9276 1879.8406
6765 940.9276 1879.8406
6766 940.9276 1879.8406
6768 940.9276 1879.8406
6769 940.9276 1879.8406
6770 940.9276 1879.8406
6773 940.9276 1879.8406
6774 940.9276 1879.8406
6775 940.9276 1879.8406
6776 940.9276 1879.8406
6777 940.9276 1879.8406
6778 940.9276 1879.8406
6779 940.9276 1879.8406
6780 940.9276 1879.8406
6781 940.9276 1879.8406
6782 940.9276 1879.8406
6783 940.9276 1879.8406
6784 940.9276 1879.8406
6785 940.9276 1879.8406
6786 940.9276 1879.8406
6787 940.9276 1879.8406
6788 940.9276 1879.8406
6789 940.9276 1879.8406
6790 940.9276 1879.8406
6791 940.9276 1879.8406
6792 940.9276 1879.8406
6793 940.9276 1879.8406
6794 940.9276 1879.8406
6795 940.9276 1879.8406
6796 940.9276 1879.8406
6797 940.9276 1879.8406
6798 940.9276 1879.8406
6799 940.9276 1879.8406
6800 940.9276 1879.8406
6801 940.9276 1879.8406
6802 940.9276 1879.8406
6803 940.9276 1879.8406
6804 940.9276 1879.8406
6805 954.4272 1906.8398
6806 954.4297 1906.8448
6807 954.4312 1906.8478
6808 954.4330 1906.8514
6809 954.4337 1906.8528
6810 954.4337 1906.8528
6811 954.4337 1906.8528
6812 954.4337 1906.8528
6813 954.4337 1906.8528
6814 954.4337 1906.8528
6815 954.4337 1906.8528
6816 954.4337 1906.8528
6817 954.4337 1906.8528
6818 954.4337 1906.8528
6819 954.4337 1906.8528
6820 954.4337 1906.8528
6822 954.4337 1906.8528
6824 954.4337 1906.8528
6825 954.4337 1906.8528
6826 954.4337 1906.8528
6827 954.4337 1906.8528
6828 954.4337 1906.8528
6829 954.4337 1906.8528
6830 954.4337 1906.8528
6833 954.4337 1906.8528
6834 954.4337 1906.8528
6835 954.4337 1906.8528
6836 954.4337 1906.8528
6837 954.4337 1906.8528
6838 954.4337 1906.8528
6839 954.4337 1906.8528
6840 954.4337 1906.8528
6841 954.4337 1906.8528
6843 954.4337 1906.8528
6844 954.4337 1906.8528
6845 954.4337 1906.8528
6848 954.4337 1906.8528
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