MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6601–6700 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6623 927.4214 1852.8282
6624 927.4214 1852.8282
6625 927.4214 1852.8282
6626 927.4214 1852.8282
6627 927.4214 1852.8282
6628 927.4214 1852.8282
6629 927.4214 1852.8282
6630 927.4214 1852.8282
6631 927.4214 1852.8282
6632 927.4214 1852.8282
6633 927.4214 1852.8282
6634 927.4214 1852.8282
6635 927.4214 1852.8282
6636 927.4214 1852.8282
6637 927.4214 1852.8282
6638 927.4214 1852.8282
6639 927.4214 1852.8282
6640 927.4242 1852.8338
6641 927.4279 1852.8412
6642 927.4333 1852.8520
6643 940.9276 1879.8406
6644 940.9276 1879.8406
6645 940.9276 1879.8406
6646 940.9276 1879.8406
6647 940.9276 1879.8406
6648 940.9276 1879.8406
6649 940.9276 1879.8406
6650 940.9276 1879.8406
6651 940.9276 1879.8406
6652 940.9276 1879.8406
6653 940.9276 1879.8406
6654 940.9276 1879.8406
6655 940.9276 1879.8406
6656 940.9276 1879.8406
6657 940.9276 1879.8406
6658 940.9276 1879.8406
6659 940.9276 1879.8406
6660 940.9276 1879.8406
6661 940.9276 1879.8406
6662 940.9276 1879.8406
6663 940.9276 1879.8406
6664 940.9276 1879.8406
6665 940.9276 1879.8406
6666 940.9276 1879.8406
6667 940.9276 1879.8406
6668 940.9276 1879.8406
6669 940.9276 1879.8406
6670 940.9276 1879.8406
6671 940.9276 1879.8406
6672 940.9276 1879.8406
6673 940.9276 1879.8406
6674 940.9276 1879.8406
6675 940.9276 1879.8406
6676 940.9276 1879.8406
6677 940.9276 1879.8406
6678 940.9276 1879.8406
6679 940.9276 1879.8406
6680 940.9276 1879.8406
6681 940.9276 1879.8406
6682 940.9276 1879.8406
6683 940.9276 1879.8406
6686 940.9276 1879.8406
6687 940.9276 1879.8406
6688 940.9276 1879.8406
6689 940.9276 1879.8406
6690 940.9276 1879.8406
6691 940.9276 1879.8406
6692 940.9276 1879.8406
6693 940.9276 1879.8406
6694 940.9276 1879.8406
6696 940.9276 1879.8406
6697 940.9276 1879.8406
6698 940.9276 1879.8406
6699 940.9276 1879.8406
6700 940.9276 1879.8406
6701 940.9276 1879.8406
6703 940.9276 1879.8406
6704 940.9276 1879.8406
6705 940.9276 1879.8406
6706 940.9276 1879.8406
6707 940.9276 1879.8406
6708 940.9276 1879.8406
6709 940.9276 1879.8406
6710 940.9276 1879.8406
6711 940.9276 1879.8406
6712 940.9276 1879.8406
6713 940.9276 1879.8406
6714 940.9276 1879.8406
6715 940.9276 1879.8406
6716 940.9276 1879.8406
6717 940.9276 1879.8406
6718 940.9276 1879.8406
6719 940.9276 1879.8406
6720 940.9276 1879.8406
6721 940.9276 1879.8406
6723 940.9276 1879.8406
6724 940.9276 1879.8406
6729 940.9276 1879.8406
6731 940.9276 1879.8406
6732 940.9276 1879.8406
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