MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6501–6600 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6496 927.4214 1852.8282
6497 927.4214 1852.8282
6498 927.4214 1852.8282
6499 927.4214 1852.8282
6500 927.4214 1852.8282
6501 927.4214 1852.8282
6502 927.4214 1852.8282
6503 927.4214 1852.8282
6504 927.4214 1852.8282
6505 927.4214 1852.8282
6506 927.4214 1852.8282
6507 927.4214 1852.8282
6508 927.4214 1852.8282
6509 927.4214 1852.8282
6510 927.4214 1852.8282
6511 927.4214 1852.8282
6512 927.4214 1852.8282
6513 927.4214 1852.8282
6514 927.4214 1852.8282
6515 927.4214 1852.8282
6516 927.4214 1852.8282
6517 927.4214 1852.8282
6518 927.4214 1852.8282
6519 927.4214 1852.8282
6520 927.4214 1852.8282
6522 927.4214 1852.8282
6523 927.4214 1852.8282
6524 927.4214 1852.8282
6526 927.4214 1852.8282
6527 927.4214 1852.8282
6528 927.4214 1852.8282
6529 927.4214 1852.8282
6530 927.4214 1852.8282
6531 927.4214 1852.8282
6532 927.4214 1852.8282
6533 927.4214 1852.8282
6534 927.4214 1852.8282
6535 927.4214 1852.8282
6536 927.4214 1852.8282
6537 927.4214 1852.8282
6539 927.4214 1852.8282
6540 927.4214 1852.8282
6541 927.4214 1852.8282
6543 927.4214 1852.8282
6544 927.4214 1852.8282
6545 927.4214 1852.8282
6547 927.4214 1852.8282
6548 927.4214 1852.8282
6550 927.4214 1852.8282
6551 927.4214 1852.8282
6552 927.4214 1852.8282
6553 927.4214 1852.8282
6555 927.4214 1852.8282
6560 927.4214 1852.8282
6561 927.4214 1852.8282
6562 927.4214 1852.8282
6564 927.4214 1852.8282
6566 927.4214 1852.8282
6567 927.4214 1852.8282
6570 927.4214 1852.8282
6571 927.4214 1852.8282
6574 927.4214 1852.8282
6575 927.4214 1852.8282
6578 927.4214 1852.8282
6579 927.4214 1852.8282
6580 927.4214 1852.8282
6583 927.4214 1852.8282
6584 927.4214 1852.8282
6585 927.4214 1852.8282
6587 927.4214 1852.8282
6589 927.4214 1852.8282
6590 927.4214 1852.8282
6591 927.4214 1852.8282
6592 927.4214 1852.8282
6593 927.4214 1852.8282
6594 927.4214 1852.8282
6595 927.4214 1852.8282
6599 927.4214 1852.8282
6600 927.4214 1852.8282
6601 927.4214 1852.8282
6602 927.4214 1852.8282
6603 927.4214 1852.8282
6604 927.4214 1852.8282
6605 927.4214 1852.8282
6606 927.4214 1852.8282
6607 927.4214 1852.8282
6608 927.4214 1852.8282
6609 927.4214 1852.8282
6610 927.4214 1852.8282
6611 927.4214 1852.8282
6612 927.4214 1852.8282
6613 927.4214 1852.8282
6614 927.4214 1852.8282
6616 927.4214 1852.8282
6617 927.4214 1852.8282
6618 927.4214 1852.8282
6619 927.4214 1852.8282
6620 927.4214 1852.8282
6621 927.4214 1852.8282
6622 927.4214 1852.8282
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