MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6301–6400 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6247 900.4092 1798.8038
6248 900.4092 1798.8038
6249 900.4092 1798.8038
6250 900.4092 1798.8038
6253 900.4092 1798.8038
6254 900.4092 1798.8038
6255 900.4092 1798.8038
6256 900.4092 1798.8038
6257 900.4092 1798.8038
6258 900.4092 1798.8038
6259 900.4092 1798.8038
6260 900.4092 1798.8038
6261 900.4092 1798.8038
6263 900.4092 1798.8038
6264 900.4092 1798.8038
6265 900.4092 1798.8038
6266 900.4092 1798.8038
6267 900.4092 1798.8038
6268 900.4092 1798.8038
6269 900.4092 1798.8038
6270 900.4092 1798.8038
6273 900.4092 1798.8038
6274 900.4092 1798.8038
6275 900.4092 1798.8038
6277 900.4092 1798.8038
6278 900.4092 1798.8038
6279 900.4092 1798.8038
6281 900.4092 1798.8038
6282 900.4092 1798.8038
6284 900.4092 1798.8038
6286 900.4092 1798.8038
6287 900.4092 1798.8038
6288 900.4092 1798.8038
6290 900.4092 1798.8038
6291 900.4092 1798.8038
6292 900.4092 1798.8038
6293 900.4092 1798.8038
6295 900.4092 1798.8038
6297 900.4092 1798.8038
6298 900.4092 1798.8038
6300 900.4092 1798.8038
6301 900.4092 1798.8038
6302 900.4092 1798.8038
6303 900.4092 1798.8038
6304 900.4092 1798.8038
6305 900.4092 1798.8038
6306 900.4092 1798.8038
6307 900.4092 1798.8038
6308 900.4092 1798.8038
6309 900.4092 1798.8038
6310 900.4092 1798.8038
6312 900.4092 1798.8038
6313 900.4092 1798.8038
6315 900.4092 1798.8038
6319 913.9153 1825.8160
6320 913.9153 1825.8160
6321 913.9153 1825.8160
6322 913.9153 1825.8160
6323 913.9153 1825.8160
6324 913.9153 1825.8160
6325 913.9153 1825.8160
6326 913.9153 1825.8160
6327 913.9153 1825.8160
6328 913.9153 1825.8160
6329 913.9153 1825.8160
6330 913.9153 1825.8160
6332 913.9153 1825.8160
6333 913.9153 1825.8160
6334 913.9153 1825.8160
6335 913.9153 1825.8160
6336 913.9153 1825.8160
6337 913.9153 1825.8160
6338 913.9153 1825.8160
6339 913.9153 1825.8160
6340 913.9153 1825.8160
6341 913.9153 1825.8160
6342 913.9153 1825.8160
6343 913.9153 1825.8160
6345 913.9153 1825.8160
6347 913.9153 1825.8160
6348 913.9153 1825.8160
6349 913.9153 1825.8160
6350 913.9153 1825.8160
6351 913.9153 1825.8160
6352 913.9153 1825.8160
6353 913.9153 1825.8160
6354 913.9153 1825.8160
6355 913.9153 1825.8160
6356 913.9153 1825.8160
6357 913.9153 1825.8160
6358 913.9153 1825.8160
6359 913.9153 1825.8160
6360 913.9153 1825.8160
6361 913.9153 1825.8160
6362 913.9153 1825.8160
6363 913.9153 1825.8160
6364 913.9153 1825.8160
6365 913.9153 1825.8160
6366 913.9153 1825.8160
6367 913.9153 1825.8160
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