MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6101–6200 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
6025 886.9030 1771.7914
6026 886.9030 1771.7914
6027 886.9030 1771.7914
6028 886.9030 1771.7914
6029 886.9030 1771.7914
6030 886.9030 1771.7914
6031 886.9030 1771.7914
6032 886.9030 1771.7914
6033 886.9030 1771.7914
6034 886.9030 1771.7914
6035 886.9030 1771.7914
6036 886.9030 1771.7914
6037 886.9030 1771.7914
6038 886.9030 1771.7914
6040 886.9030 1771.7914
6041 886.9030 1771.7914
6042 886.9030 1771.7914
6043 886.9030 1771.7914
6044 886.9030 1771.7914
6045 886.9030 1771.7914
6046 886.9030 1771.7914
6047 886.9030 1771.7914
6048 886.9030 1771.7914
6049 886.9030 1771.7914
6050 886.9030 1771.7914
6051 886.9030 1771.7914
6052 886.9030 1771.7914
6053 886.9030 1771.7914
6054 886.9030 1771.7914
6055 886.9030 1771.7914
6056 886.9030 1771.7914
6057 886.9030 1771.7914
6058 886.9030 1771.7914
6059 886.9030 1771.7914
6060 886.9030 1771.7914
6061 886.9030 1771.7914
6062 886.9030 1771.7914
6063 886.9030 1771.7914
6064 886.9030 1771.7914
6065 886.9030 1771.7914
6066 886.9030 1771.7914
6068 886.9030 1771.7914
6069 886.9030 1771.7914
6071 886.9030 1771.7914
6073 886.9030 1771.7914
6074 886.9030 1771.7914
6075 886.9030 1771.7914
6076 886.9030 1771.7914
6077 886.9030 1771.7914
6078 886.9030 1771.7914
6079 886.9030 1771.7914
6080 886.9030 1771.7914
6081 886.9030 1771.7914
6083 886.9030 1771.7914
6084 886.9030 1771.7914
6086 886.9030 1771.7914
6088 886.9030 1771.7914
6089 886.9030 1771.7914
6090 886.9030 1771.7914
6091 886.9030 1771.7914
6092 886.9030 1771.7914
6094 886.9030 1771.7914
6095 886.9030 1771.7914
6096 886.9030 1771.7914
6097 886.9030 1771.7914
6098 886.9030 1771.7914
6100 886.9030 1771.7914
6101 886.9030 1771.7914
6102 886.9030 1771.7914
6103 886.9030 1771.7914
6104 886.9030 1771.7914
6105 886.9030 1771.7914
6106 886.9030 1771.7914
6107 886.9030 1771.7914
6108 886.9030 1771.7914
6109 886.9030 1771.7914
6110 886.9030 1771.7914
6111 886.9030 1771.7914
6112 886.9030 1771.7914
6114 886.9030 1771.7914
6115 886.9030 1771.7914
6116 886.9030 1771.7914
6117 886.9030 1771.7914
6119 886.9030 1771.7914
6120 886.9030 1771.7914
6121 886.9030 1771.7914
6122 886.9030 1771.7914
6123 886.9030 1771.7914
6124 886.9030 1771.7914
6125 886.9030 1771.7914
6126 886.9030 1771.7914
6127 886.9030 1771.7914
6128 886.9030 1771.7914
6129 886.9030 1771.7914
6130 886.9030 1771.7914
6131 886.9030 1771.7914
6132 886.9030 1771.7914
6133 886.9030 1771.7914
6134 886.9030 1771.7914
6135 886.9030 1771.7914
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