MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 6001–6100 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5904 873.3969 1744.7792
5905 873.3969 1744.7792
5907 873.3969 1744.7792
5908 873.3969 1744.7792
5909 873.3969 1744.7792
5911 873.3969 1744.7792
5912 873.3969 1744.7792
5915 873.3969 1744.7792
5916 873.3969 1744.7792
5917 873.3969 1744.7792
5919 873.3969 1744.7792
5920 873.3969 1744.7792
5921 873.3969 1744.7792
5924 873.3969 1744.7792
5925 873.3969 1744.7792
5926 873.3969 1744.7792
5927 873.3969 1744.7792
5928 873.3969 1744.7792
5929 873.3969 1744.7792
5931 873.3969 1744.7792
5932 873.3969 1744.7792
5934 873.3969 1744.7792
5935 873.3969 1744.7792
5936 873.3969 1744.7792
5937 873.3969 1744.7792
5938 873.3969 1744.7792
5939 873.3969 1744.7792
5941 873.3969 1744.7792
5942 873.3969 1744.7792
5943 873.3969 1744.7792
5945 873.3969 1744.7792
5946 873.3969 1744.7792
5947 873.3969 1744.7792
5948 873.3969 1744.7792
5950 873.3969 1744.7792
5951 873.3969 1744.7792
5952 873.3969 1744.7792
5953 873.3969 1744.7792
5954 873.3969 1744.7792
5955 873.3969 1744.7792
5956 873.3969 1744.7792
5957 873.3969 1744.7792
5958 873.3969 1744.7792
5959 873.3969 1744.7792
5960 873.3969 1744.7792
5961 873.3969 1744.7792
5963 873.3969 1744.7792
5964 873.3969 1744.7792
5965 873.3969 1744.7792
5966 873.3969 1744.7792
5967 873.3969 1744.7792
5968 873.3969 1744.7792
5969 873.3969 1744.7792
5970 873.3969 1744.7792
5972 873.3969 1744.7792
5973 873.3969 1744.7792
5974 873.3969 1744.7792
5975 873.3969 1744.7792
5976 873.3969 1744.7792
5977 873.3969 1744.7792
5978 873.3969 1744.7792
5979 873.3969 1744.7792
5980 873.3969 1744.7792
5981 873.3969 1744.7792
5982 873.3969 1744.7792
5984 873.3974 1744.7802
5985 873.3981 1744.7816
5987 873.4005 1744.7864
5990 873.4048 1744.7950
5991 873.4064 1744.7982
5993 873.4078 1744.8010
5994 873.4099 1744.8052
5995 886.8901 1771.7656
5996 886.8931 1771.7716
5997 886.8984 1771.7822
5998 886.9022 1771.7898
5999 886.9026 1771.7906
6000 886.9030 1771.7914
6001 886.9030 1771.7914
6002 886.9030 1771.7914
6003 886.9030 1771.7914
6004 886.9030 1771.7914
6005 886.9030 1771.7914
6006 886.9030 1771.7914
6008 886.9030 1771.7914
6009 886.9030 1771.7914
6010 886.9030 1771.7914
6011 886.9030 1771.7914
6012 886.9030 1771.7914
6013 886.9030 1771.7914
6014 886.9030 1771.7914
6015 886.9030 1771.7914
6016 886.9030 1771.7914
6017 886.9030 1771.7914
6019 886.9030 1771.7914
6020 886.9030 1771.7914
6021 886.9030 1771.7914
6022 886.9030 1771.7914
6023 886.9030 1771.7914
6024 886.9030 1771.7914
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