MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5901–6000 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5795 859.8907 1717.7668
5798 859.8907 1717.7668
5799 859.8907 1717.7668
5800 859.8907 1717.7668
5801 859.8907 1717.7668
5802 859.8907 1717.7668
5803 859.8907 1717.7668
5804 859.8907 1717.7668
5805 859.8907 1717.7668
5807 859.8907 1717.7668
5808 859.8907 1717.7668
5809 859.8907 1717.7668
5810 859.8907 1717.7668
5811 859.8907 1717.7668
5812 859.8907 1717.7668
5813 859.8907 1717.7668
5814 859.8907 1717.7668
5815 859.8907 1717.7668
5816 859.8907 1717.7668
5817 859.8907 1717.7668
5818 859.8907 1717.7668
5819 859.8907 1717.7668
5820 859.8907 1717.7668
5821 859.8907 1717.7668
5822 859.8907 1717.7668
5823 859.8907 1717.7668
5824 859.8907 1717.7668
5825 859.8907 1717.7668
5826 859.8907 1717.7668
5827 859.8907 1717.7668
5828 859.8907 1717.7668
5829 859.8907 1717.7668
5830 859.8907 1717.7668
5831 859.8987 1717.7828
5832 859.9025 1717.7904
5833 873.3957 1744.7768
5834 873.3964 1744.7782
5836 873.3969 1744.7792
5837 873.3969 1744.7792
5838 873.3969 1744.7792
5839 873.3969 1744.7792
5840 873.3969 1744.7792
5841 873.3969 1744.7792
5842 873.3969 1744.7792
5843 873.3969 1744.7792
5844 873.3969 1744.7792
5845 873.3969 1744.7792
5846 873.3969 1744.7792
5847 873.3969 1744.7792
5848 873.3969 1744.7792
5849 873.3969 1744.7792
5850 873.3969 1744.7792
5851 873.3969 1744.7792
5852 873.3969 1744.7792
5853 873.3969 1744.7792
5854 873.3969 1744.7792
5855 873.3969 1744.7792
5856 873.3969 1744.7792
5857 873.3969 1744.7792
5858 873.3969 1744.7792
5859 873.3969 1744.7792
5861 873.3969 1744.7792
5862 873.3969 1744.7792
5863 873.3969 1744.7792
5864 873.3969 1744.7792
5865 873.3969 1744.7792
5866 873.3969 1744.7792
5867 873.3969 1744.7792
5868 873.3969 1744.7792
5869 873.3969 1744.7792
5870 873.3969 1744.7792
5871 873.3969 1744.7792
5872 873.3969 1744.7792
5873 873.3969 1744.7792
5874 873.3969 1744.7792
5875 873.3969 1744.7792
5877 873.3969 1744.7792
5878 873.3969 1744.7792
5879 873.3969 1744.7792
5880 873.3969 1744.7792
5881 873.3969 1744.7792
5882 873.3969 1744.7792
5883 873.3969 1744.7792
5884 873.3969 1744.7792
5885 873.3969 1744.7792
5886 873.3969 1744.7792
5887 873.3969 1744.7792
5888 873.3969 1744.7792
5889 873.3969 1744.7792
5890 873.3969 1744.7792
5891 873.3969 1744.7792
5892 873.3969 1744.7792
5893 873.3969 1744.7792
5894 873.3969 1744.7792
5895 873.3969 1744.7792
5896 873.3969 1744.7792
5898 873.3969 1744.7792
5899 873.3969 1744.7792
5900 873.3969 1744.7792
5901 873.3969 1744.7792
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