MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4423 751.8416 1501.6686 1501.6943 -17.1 1 2 1.9 +1Score > 17 indicates identity EKMQTNPQNQQR + Deamidated (NQ)
6572 927.4214 1852.8282 1852.8241 2.25 1 2 1.8 +1Score > 17 indicates identity EWYEKHGNSHQGEPR
6727 940.9276 1879.8406 1879.8469 -3.33 0 2 2.1 +1Score > 17 indicates identity ISGADLMEGSSTAEEVQR + Deamidated (NQ)
2947 630.2831 1258.5516 1258.5621 -8.27 0 2 0.71 +1Score > 13 indicates identity ISMMPGMPSHR + Oxidation (M)
3314 657.2986 1312.5826 1312.5830 -0.24 0 2 1.6 +1Score > 16 indicates identity NHCEILQGNAR + 2 Deamidated (NQ)
1722 522.2372 1042.4598 1042.4727 -12.3 1 2 0.71 +1Score > 13 indicates identity ARHGDAGGMR + Oxidation (M)
4282 738.3355 1474.6564 1474.6524 2.76 1 2 1.4 +1Score > 15 indicates identity NMAWRHNPNYR + Deamidated (NQ); Oxidation (M)
4279 738.3355 1474.6564 1474.6663 -6.70 0 1 1.4 +1Score > 15 indicates identity SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M)
4656 765.3478 1528.6810 1528.7005 -12.7 0 1 1.1 +1Score > 15 indicates identity EVPGVQLTNEGDNR + 2 Deamidated (NQ)
4784 778.8539 1555.6932 1555.7163 -14.8 0 1 1.3 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
141 387.1808 772.3470 772.3351 15.4 0 1 0.71 +1Score > 13 indicates identity DPNGDVR + Deamidated (NQ)
1057 468.2127 934.4108 934.4178 -7.46 1 1 1.4 +1Score > 15 indicates identity LDEDCKR
2845 616.7802 1231.5458 1231.5431 2.24 0 1 1.2 +1Score > 15 indicates identity MEDETLGFFK + Oxidation (M)
2976 630.2844 1258.5542 1258.5367 14.0 0 1 0.75 +1Score > 13 indicates identity QWNNEPAGWR + 2 Deamidated (NQ)
3304 657.2986 1312.5826 1312.5830 -0.24 0 1 1.6 +1Score > 16 indicates identity NHCEILQGNAR + 2 Deamidated (NQ)
3312 657.2986 1312.5826 1312.5571 19.4 0 1 1.6 +1Score > 16 indicates identity YGDAFTQPEQR + 2 Deamidated (NQ)
3641 684.3109 1366.6072 1366.6299 -16.6 1 1 1.6 +1Score > 16 indicates identity SRDAFIDEMQR
4683 765.3478 1528.6810 1528.6576 15.3 0 1 1.1 +1Score > 15 indicates identity HQEMGQPNVSTER + Deamidated (NQ); Oxidation (M)
6764 940.9276 1879.8406 1879.8469 -3.35 0 1 2.1 +1Score > 17 indicates identity NEVVADGNDSVTMTATVR + 2 Deamidated (NQ)
3650 684.3109 1366.6072 1366.6266 -14.1 1 1 1.6 +1Score > 16 indicates identity DWRETNIDYR
4105 724.8293 1447.6440 1447.6725 -19.7 1 1 0.89 +1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
4315 738.3355 1474.6564 1474.6663 -6.70 0 1 1.4 +1Score > 15 indicates identity SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M)
6598 927.4214 1852.8282 1852.8326 -2.36 1 1 1.8 +1Score > 17 indicates identity TLNSNQFDNEDAIKDK + 2 Deamidated (NQ)
4018 711.3232 1420.6318 1420.6252 4.68 1 1 1.8 +1Score > 16 indicates identity ANNAINDMVRQR + 4 Deamidated (NQ); Oxidation (M)
6722 940.9276 1879.8406 1879.8258 7.90 0 1 2.1 +1Score > 17 indicates identity MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M)
995 468.2127 934.4108 934.4178 -7.47 0 1 1.4 +1Score > 15 indicates identity MNVGDVQR + Deamidated (NQ); Oxidation (M)
6582 927.4214 1852.8282 1852.8241 2.25 1 1 1.8 +1Score > 17 indicates identity EWYEKHGNSHQGEPR
4977 792.3600 1582.7054 1582.6875 11.4 0 1 1.2 +1Score > 15 indicates identity VYFCDPQSPWQR + Deamidated (NQ)
98 387.1800 772.3454 772.3537 -10.7 1 1 0.72 +1Score > 13 indicates identity HKDMNK + Deamidated (NQ)
2461 589.7679 1177.5212 1177.5332 -10.1 1 1 1 +1Score > 14 indicates identity ANNPTSRMMR + Deamidated (NQ)
4362 738.3355 1474.6564 1474.6728 -11.1 0 1 1.4 +1Score > 15 indicates identity NQYYGITAGPAYR + 2 Deamidated (NQ)
7101 967.9399 1933.8652 1933.8993 -17.6 1 1 1.9 +1Score > 17 indicates identity LSGFDMGYSGIQFGGDKR
1034 468.2127 934.4108 934.4178 -7.46 1 1 1.4 +1Score > 15 indicates identity LDEDCKR
4142 724.8293 1447.6440 1447.6725 -19.7 1 1 0.9 +1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
1117 468.2127 934.4108 934.4178 -7.46 1 1 1.4 +1Score > 15 indicates identity LDEDCKR
1182 481.7188 961.4230 961.4253 -2.38 0 1 1.2 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
5774 859.8907 1717.7668 1717.7803 -7.85 1 1 1.3 +1Score > 15 indicates identity FMAQYQQMKAQGIR + 3 Deamidated (NQ); Oxidation (M)
5988 873.4007 1744.7868 1744.7735 7.63 0 1 2.6 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
2654 603.2741 1204.5336 1204.5394 -4.77 0 1 1.6 +1Score > 16 indicates identity DDPSMVADLSR
2989 630.2849 1258.5552 1258.5789 -18.8 0 1 0.76 +1Score > 13 indicates identity DDGLILNDNGGR + Deamidated (NQ)
4455 751.8416 1501.6686 1501.6727 -2.71 1 1 2 +1Score > 17 indicates identity RMPAMMNYTAGVK + Deamidated (NQ); 2 Oxidation (M)
5902 873.3969 1744.7792 1744.7904 -6.37 0 1 2.5 +1Score > 18 indicates identity ELNNPEHDSYAISEK
6429 913.9153 1825.8160 1825.8451 -15.9 1 1 2 +1Score > 17 indicates identity ASGGFAMLAVDQREAMR + Deamidated (NQ); Oxidation (M)
1031 468.2127 934.4108 934.4178 -7.46 1 1 1.4 +1Score > 15 indicates identity LDEDCKR
6387 913.9153 1825.8160 1825.8372 -11.6 0 1 2 +1Score > 17 indicates identity AMLTLMLQAMGQADAGR + Deamidated (NQ); 3 Oxidation (M)
6558 927.4214 1852.8282 1852.8625 -18.5 1 1 1.9 +1Score > 17 indicates identity LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
2318 576.2618 1150.5090 1150.5254 -14.2 1 1 1.4 +1Score > 15 indicates identity LEFNNDNRK + 2 Deamidated (NQ)
1828 535.7434 1069.4722 1069.4862 -13.1 0 1 0.73 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
1055 468.2127 934.4108 934.4178 -7.46 1 1 1.4 +1Score > 15 indicates identity LDEDCKR
836 454.7066 907.3986 907.3817 18.6 1 1 1 +1Score > 14 indicates identity RDNQAMR + 2 Deamidated (NQ); Oxidation (M)
6414 913.9153 1825.8160 1825.8186 -1.39 1 1 2.1 +1Score > 17 indicates identity ERQTGMQMVQSVLER + 3 Deamidated (NQ); 2 Oxidation (M)
1974 549.2496 1096.4846 1096.4892 -4.19 1 1 1.3 +1Score > 15 indicates identity EEMKQIMR + Deamidated (NQ); 2 Oxidation (M)
3564 670.8048 1339.5950 1339.6112 -12.0 0 1 1.8 +1Score > 16 indicates identity ASVSMQMGIQVR + 2 Deamidated (NQ); 2 Oxidation (M)
6285 900.4092 1798.8038 1798.7870 9.35 0 1 1.3 +1Score > 15 indicates identity TQADAWDINQGHQSAR + 2 Deamidated (NQ)
3795 697.8171 1393.6196 1393.6031 11.9 0 1 1.8 +1Score > 16 indicates identity TEQVNQQLMER + 3 Deamidated (NQ); Oxidation (M)
1162 481.7188 961.4230 961.4253 -2.38 0 1 1.2 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
6070 886.9030 1771.7914 1771.7725 10.7 1 1 1.7 +1Score > 16 indicates identity QEVCAMMALNMLRR + 2 Deamidated (NQ); 3 Oxidation (M)
649 441.1924 880.3702 880.3562 15.9 0 1 0.98 +1Score > 14 indicates identity EDEAFNR + Deamidated (NQ)
3071 630.2864 1258.5582 1258.5724 -11.3 1 1 1 +1Score > 14 indicates identity VNDRSHQMEK + Oxidation (M)
5835 873.3965 1744.7784 1744.7947 -9.29 0 1 2.6 +1Score > 18 indicates identity MIGPNCPGVITPGECK + Oxidation (M)
5403 832.8785 1663.7424 1663.7359 3.94 0 1 1.6 +1Score > 16 indicates identity AMEDQIINDLNDTR + Deamidated (NQ); Oxidation (M)
3318 657.2986 1312.5826 1312.5830 -0.24 0 1 1.7 +1Score > 16 indicates identity NHCEILQGNAR + 2 Deamidated (NQ)
3773 697.8171 1393.6196 1393.6329 -9.53 1 1 1.8 +1Score > 16 indicates identity EEMATEMERLR
6397 913.9153 1825.8160 1825.8482 -17.6 1 1 2.1 +1Score > 17 indicates identity LAELQYARNDQAFQR + 4 Deamidated (NQ)
2865 616.7802 1231.5458 1231.5680 -18.0 0 1 1.3 +1Score > 15 indicates identity TDAEQAQELAR + Deamidated (NQ)
1209 481.7188 961.4230 961.4253 -2.38 0 1 1.2 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
5274 819.3723 1636.7300 1636.7119 11.1 1 1 1.9 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
6118 886.9030 1771.7914 1771.7968 -3.04 0 1 1.7 +1Score > 16 indicates identity SQSTVGAGDSMVGAMTLK + Deamidated (NQ); 2 Oxidation (M)
2927 630.2807 1258.5468 1258.5621 -12.1 0 1 0.76 +1Score > 13 indicates identity ISMMPGMPSHR + Oxidation (M)
2026 549.2496 1096.4846 1096.4819 2.52 1 1 1.3 +1Score > 15 indicates identity MSGGETDTRK + Oxidation (M)
2150 562.7557 1123.4968 1123.4894 6.65 0 1 1.2 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
5586 846.3846 1690.7546 1690.7475 4.25 0 1 1.6 +1Score > 16 indicates identity ESSVPFSYYDNQQK
6417 913.9153 1825.8160 1825.8186 -1.39 1 1 2.1 +1Score > 17 indicates identity ERQTGMQMVQSVLER + 3 Deamidated (NQ); 2 Oxidation (M)
1069 468.2127 934.4108 934.4178 -7.46 1 1 1.5 +1Score > 15 indicates identity LDEDCKR
1832 535.7434 1069.4722 1069.4862 -13.1 0 1 0.77 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
1935 535.7434 1069.4722 1069.4902 -16.8 0 1 0.77 +1Score > 13 indicates identity YQLNMWSK + Deamidated (NQ)
6067 886.9030 1771.7914 1771.7725 10.7 1 1 1.7 +1Score > 16 indicates identity QEVCAMMALNMLRR + 2 Deamidated (NQ); 3 Oxidation (M)
6899 954.4337 1906.8528 1906.8652 -6.47 0 1 2.2 +1Score > 17 indicates identity ATIDGLENMNSPEMVAAK + Deamidated (NQ); Oxidation (M)
1838 535.7434 1069.4722 1069.4862 -13.1 0 1 0.77 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
2871 616.7802 1231.5458 1231.5503 -3.62 0 1 1.4 +1Score > 15 indicates identity MTDLHTDVER + Oxidation (M)
4451 751.8416 1501.6686 1501.6891 -13.6 1 1 2.1 +1Score > 17 indicates identity WEWSRYYAWR
4645 765.3478 1528.6810 1528.6576 15.3 0 1 1.2 +1Score > 15 indicates identity HQEMGQPNVSTER + Deamidated (NQ); Oxidation (M)
2410 576.2618 1150.5090 1150.5288 -17.2 0 1 1.4 +1Score > 15 indicates identity TGDIMTIGGNR + Deamidated (NQ); Oxidation (M)
4305 738.3355 1474.6564 1474.6663 -6.70 0 1 1.5 +1Score > 15 indicates identity SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M)
1063 468.2127 934.4108 934.4290 -19.5 1 1 1.5 +1Score > 15 indicates identity LNRSCER + Deamidated (NQ)
5906 873.3969 1744.7792 1744.7735 3.28 0 1 2.7 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
5759 859.8907 1717.7668 1717.7876 -12.1 1 1 1.4 +1Score > 15 indicates identity CRAEEPALNMLADGR + Oxidation (M)
5940 873.3969 1744.7792 1744.7978 -10.6 1 1 2.7 +1Score > 18 indicates identity NFMENVLRYLSDDK + 2 Deamidated (NQ)
6400 913.9153 1825.8160 1825.8186 -1.39 1 1 2.2 +1Score > 17 indicates identity ERQTGMQMVQSVLER + 3 Deamidated (NQ); 2 Oxidation (M)
3834 697.8171 1393.6196 1393.6474 -19.9 1 1 1.9 +1Score > 16 indicates identity NVDKQTQDFAAR + 2 Deamidated (NQ)
6880 954.4337 1906.8528 1906.8784 -13.4 0 1 2.3 +1Score > 17 indicates identity MSQFFYIHPDNPQQR
2803 616.7802 1231.5458 1231.5655 -16.0 1 1 1.4 +1Score > 15 indicates identity EMREIYYGR + Oxidation (M)
2992 630.2850 1258.5554 1258.5621 -5.25 0 1 0.83 +1Score > 13 indicates identity ISMMPGMPSHR + Oxidation (M)
5784 859.8907 1717.7668 1717.7876 -12.1 1 1 1.5 +1Score > 15 indicates identity CRAEEPALNMLADGR + Oxidation (M)
7116 967.9399 1933.8652 1933.8312 17.6 0 1 2.1 +1Score > 17 indicates identity HQMSFDNGMNWNNALR
4505 751.8416 1501.6686 1501.6772 -5.69 0 1 2.1 +1Score > 17 indicates identity YHQWMNELPER
7102 967.9399 1933.8652 1933.8992 -17.6 1 1 2.1 +1Score > 17 indicates identity MSTPDNRSVNFFSLFR + Deamidated (NQ); Oxidation (M)
2371 576.2618 1150.5090 1150.5117 -2.32 0 1 1.5 +1Score > 15 indicates identity YMTWDNPPK
2931 630.2814 1258.5482 1258.5621 -11.0 0 1 0.79 +1Score > 13 indicates identity ISMMPGMPSHR + Oxidation (M)
4295 738.3355 1474.6564 1474.6663 -6.70 0 1 1.5 +1Score > 15 indicates identity SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M)
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