MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5801–5900 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5681 859.8907 1717.7668
5682 859.8907 1717.7668
5683 859.8907 1717.7668
5684 859.8907 1717.7668
5685 859.8907 1717.7668
5686 859.8907 1717.7668
5687 859.8907 1717.7668
5688 859.8907 1717.7668
5689 859.8907 1717.7668
5690 859.8907 1717.7668
5691 859.8907 1717.7668
5692 859.8907 1717.7668
5693 859.8907 1717.7668
5694 859.8907 1717.7668
5695 859.8907 1717.7668
5696 859.8907 1717.7668
5697 859.8907 1717.7668
5698 859.8907 1717.7668
5699 859.8907 1717.7668
5700 859.8907 1717.7668
5701 859.8907 1717.7668
5702 859.8907 1717.7668
5703 859.8907 1717.7668
5704 859.8907 1717.7668
5705 859.8907 1717.7668
5706 859.8907 1717.7668
5707 859.8907 1717.7668
5708 859.8907 1717.7668
5709 859.8907 1717.7668
5710 859.8907 1717.7668
5711 859.8907 1717.7668
5712 859.8907 1717.7668
5713 859.8907 1717.7668
5714 859.8907 1717.7668
5715 859.8907 1717.7668
5716 859.8907 1717.7668
5717 859.8907 1717.7668
5718 859.8907 1717.7668
5719 859.8907 1717.7668
5720 859.8907 1717.7668
5721 859.8907 1717.7668
5722 859.8907 1717.7668
5723 859.8907 1717.7668
5724 859.8907 1717.7668
5725 859.8907 1717.7668
5726 859.8907 1717.7668
5727 859.8907 1717.7668
5729 859.8907 1717.7668
5730 859.8907 1717.7668
5731 859.8907 1717.7668
5732 859.8907 1717.7668
5733 859.8907 1717.7668
5734 859.8907 1717.7668
5735 859.8907 1717.7668
5738 859.8907 1717.7668
5739 859.8907 1717.7668
5740 859.8907 1717.7668
5741 859.8907 1717.7668
5743 859.8907 1717.7668
5744 859.8907 1717.7668
5745 859.8907 1717.7668
5747 859.8907 1717.7668
5748 859.8907 1717.7668
5749 859.8907 1717.7668
5750 859.8907 1717.7668
5751 859.8907 1717.7668
5752 859.8907 1717.7668
5753 859.8907 1717.7668
5754 859.8907 1717.7668
5755 859.8907 1717.7668
5756 859.8907 1717.7668
5757 859.8907 1717.7668
5758 859.8907 1717.7668
5760 859.8907 1717.7668
5761 859.8907 1717.7668
5763 859.8907 1717.7668
5764 859.8907 1717.7668
5765 859.8907 1717.7668
5766 859.8907 1717.7668
5767 859.8907 1717.7668
5769 859.8907 1717.7668
5770 859.8907 1717.7668
5771 859.8907 1717.7668
5772 859.8907 1717.7668
5773 859.8907 1717.7668
5776 859.8907 1717.7668
5777 859.8907 1717.7668
5778 859.8907 1717.7668
5779 859.8907 1717.7668
5780 859.8907 1717.7668
5781 859.8907 1717.7668
5782 859.8907 1717.7668
5785 859.8907 1717.7668
5787 859.8907 1717.7668
5788 859.8907 1717.7668
5790 859.8907 1717.7668
5791 859.8907 1717.7668
5792 859.8907 1717.7668
5793 859.8907 1717.7668
5794 859.8907 1717.7668
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