MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5701–5800 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5563 846.3846 1690.7546
5564 846.3846 1690.7546
5565 846.3846 1690.7546
5566 846.3846 1690.7546
5567 846.3846 1690.7546
5568 846.3846 1690.7546
5569 846.3846 1690.7546
5570 846.3846 1690.7546
5571 846.3846 1690.7546
5572 846.3846 1690.7546
5573 846.3846 1690.7546
5574 846.3846 1690.7546
5576 846.3846 1690.7546
5577 846.3846 1690.7546
5578 846.3846 1690.7546
5580 846.3846 1690.7546
5581 846.3846 1690.7546
5582 846.3846 1690.7546
5585 846.3846 1690.7546
5589 846.3846 1690.7546
5590 846.3846 1690.7546
5591 846.3846 1690.7546
5593 846.3846 1690.7546
5594 846.3846 1690.7546
5595 846.3846 1690.7546
5596 846.3846 1690.7546
5598 846.3846 1690.7546
5600 846.3846 1690.7546
5601 846.3846 1690.7546
5602 846.3846 1690.7546
5604 846.3846 1690.7546
5607 846.3846 1690.7546
5609 846.3846 1690.7546
5610 846.3846 1690.7546
5611 846.3846 1690.7546
5612 846.3846 1690.7546
5613 846.3846 1690.7546
5614 846.3846 1690.7546
5616 846.3846 1690.7546
5617 846.3846 1690.7546
5619 846.3846 1690.7546
5620 846.3846 1690.7546
5621 846.3846 1690.7546
5622 846.3846 1690.7546
5623 846.3846 1690.7546
5624 846.3846 1690.7546
5625 846.3846 1690.7546
5626 846.3846 1690.7546
5627 846.3846 1690.7546
5628 846.3846 1690.7546
5629 846.3846 1690.7546
5630 846.3846 1690.7546
5631 846.3846 1690.7546
5632 846.3846 1690.7546
5633 846.3846 1690.7546
5634 846.3846 1690.7546
5635 846.3846 1690.7546
5636 846.3846 1690.7546
5637 846.3846 1690.7546
5638 846.3846 1690.7546
5639 846.3846 1690.7546
5640 846.3846 1690.7546
5641 846.3846 1690.7546
5642 846.3846 1690.7546
5643 846.3846 1690.7546
5644 846.3846 1690.7546
5645 846.3846 1690.7546
5646 846.3846 1690.7546
5647 846.3846 1690.7546
5648 846.3846 1690.7546
5649 846.3846 1690.7546
5650 846.3846 1690.7546
5652 846.3846 1690.7546
5653 846.3846 1690.7546
5654 846.3846 1690.7546
5655 846.3846 1690.7546
5657 846.3879 1690.7612
5658 846.3889 1690.7632
5659 846.3893 1690.7640
5660 846.3902 1690.7658
5661 846.3911 1690.7676
5662 846.3914 1690.7682
5663 846.3917 1690.7688
5664 846.3920 1690.7694
5665 846.3923 1690.7700
5666 846.3925 1690.7704
5667 846.3925 1690.7704
5668 846.3931 1690.7716
5669 846.3932 1690.7718
5670 846.4015 1690.7884
5671 859.8907 1717.7668
5672 859.8907 1717.7668
5673 859.8907 1717.7668
5674 859.8907 1717.7668
5675 859.8907 1717.7668
5676 859.8907 1717.7668
5677 859.8907 1717.7668
5678 859.8907 1717.7668
5679 859.8907 1717.7668
5680 859.8907 1717.7668
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