MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5601–5700 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5457 832.8785 1663.7424
5458 832.8785 1663.7424
5459 832.8785 1663.7424
5460 832.8785 1663.7424
5461 832.8785 1663.7424
5462 832.8785 1663.7424
5463 832.8785 1663.7424
5464 832.8785 1663.7424
5466 832.8785 1663.7424
5467 832.8785 1663.7424
5468 832.8785 1663.7424
5469 832.8785 1663.7424
5470 832.8785 1663.7424
5471 832.8785 1663.7424
5472 832.8785 1663.7424
5473 832.8785 1663.7424
5474 832.8785 1663.7424
5475 832.8785 1663.7424
5476 832.8785 1663.7424
5478 832.8785 1663.7424
5479 832.8785 1663.7424
5480 832.8785 1663.7424
5481 832.8785 1663.7424
5482 832.8785 1663.7424
5483 832.8785 1663.7424
5484 832.8785 1663.7424
5485 832.8785 1663.7424
5486 832.8785 1663.7424
5487 832.8785 1663.7424
5488 832.8785 1663.7424
5489 832.8785 1663.7424
5490 832.8785 1663.7424
5491 832.8785 1663.7424
5492 832.8785 1663.7424
5493 832.8785 1663.7424
5494 832.8785 1663.7424
5495 832.8785 1663.7424
5496 832.8785 1663.7424
5497 832.8785 1663.7424
5498 832.8785 1663.7424
5499 832.8785 1663.7424
5500 832.8785 1663.7424
5501 832.8785 1663.7424
5502 832.8785 1663.7424
5503 832.8785 1663.7424
5504 832.8785 1663.7424
5505 832.8785 1663.7424
5506 832.8785 1663.7424
5507 832.8844 1663.7542
5508 832.8927 1663.7708
5510 846.3808 1690.7470
5511 846.3828 1690.7510
5512 846.3846 1690.7546
5513 846.3846 1690.7546
5514 846.3846 1690.7546
5515 846.3846 1690.7546
5516 846.3846 1690.7546
5517 846.3846 1690.7546
5518 846.3846 1690.7546
5519 846.3846 1690.7546
5520 846.3846 1690.7546
5521 846.3846 1690.7546
5522 846.3846 1690.7546
5523 846.3846 1690.7546
5524 846.3846 1690.7546
5525 846.3846 1690.7546
5526 846.3846 1690.7546
5527 846.3846 1690.7546
5528 846.3846 1690.7546
5529 846.3846 1690.7546
5530 846.3846 1690.7546
5532 846.3846 1690.7546
5533 846.3846 1690.7546
5534 846.3846 1690.7546
5535 846.3846 1690.7546
5536 846.3846 1690.7546
5537 846.3846 1690.7546
5538 846.3846 1690.7546
5539 846.3846 1690.7546
5540 846.3846 1690.7546
5541 846.3846 1690.7546
5542 846.3846 1690.7546
5543 846.3846 1690.7546
5544 846.3846 1690.7546
5545 846.3846 1690.7546
5546 846.3846 1690.7546
5547 846.3846 1690.7546
5548 846.3846 1690.7546
5549 846.3846 1690.7546
5550 846.3846 1690.7546
5551 846.3846 1690.7546
5553 846.3846 1690.7546
5554 846.3846 1690.7546
5555 846.3846 1690.7546
5556 846.3846 1690.7546
5557 846.3846 1690.7546
5558 846.3846 1690.7546
5560 846.3846 1690.7546
5561 846.3846 1690.7546
5562 846.3846 1690.7546
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