MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5501–5600 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5350 832.8785 1663.7424
5351 832.8785 1663.7424
5352 832.8785 1663.7424
5353 832.8785 1663.7424
5354 832.8785 1663.7424
5355 832.8785 1663.7424
5356 832.8785 1663.7424
5357 832.8785 1663.7424
5358 832.8785 1663.7424
5359 832.8785 1663.7424
5360 832.8785 1663.7424
5362 832.8785 1663.7424
5363 832.8785 1663.7424
5364 832.8785 1663.7424
5365 832.8785 1663.7424
5366 832.8785 1663.7424
5367 832.8785 1663.7424
5368 832.8785 1663.7424
5369 832.8785 1663.7424
5370 832.8785 1663.7424
5371 832.8785 1663.7424
5372 832.8785 1663.7424
5373 832.8785 1663.7424
5374 832.8785 1663.7424
5375 832.8785 1663.7424
5376 832.8785 1663.7424
5377 832.8785 1663.7424
5378 832.8785 1663.7424
5379 832.8785 1663.7424
5380 832.8785 1663.7424
5381 832.8785 1663.7424
5382 832.8785 1663.7424
5383 832.8785 1663.7424
5384 832.8785 1663.7424
5385 832.8785 1663.7424
5386 832.8785 1663.7424
5387 832.8785 1663.7424
5388 832.8785 1663.7424
5389 832.8785 1663.7424
5390 832.8785 1663.7424
5391 832.8785 1663.7424
5392 832.8785 1663.7424
5393 832.8785 1663.7424
5394 832.8785 1663.7424
5395 832.8785 1663.7424
5396 832.8785 1663.7424
5397 832.8785 1663.7424
5398 832.8785 1663.7424
5401 832.8785 1663.7424
5402 832.8785 1663.7424
5404 832.8785 1663.7424
5405 832.8785 1663.7424
5406 832.8785 1663.7424
5407 832.8785 1663.7424
5408 832.8785 1663.7424
5410 832.8785 1663.7424
5411 832.8785 1663.7424
5412 832.8785 1663.7424
5413 832.8785 1663.7424
5414 832.8785 1663.7424
5415 832.8785 1663.7424
5416 832.8785 1663.7424
5417 832.8785 1663.7424
5418 832.8785 1663.7424
5419 832.8785 1663.7424
5421 832.8785 1663.7424
5422 832.8785 1663.7424
5423 832.8785 1663.7424
5424 832.8785 1663.7424
5425 832.8785 1663.7424
5426 832.8785 1663.7424
5427 832.8785 1663.7424
5428 832.8785 1663.7424
5429 832.8785 1663.7424
5430 832.8785 1663.7424
5432 832.8785 1663.7424
5433 832.8785 1663.7424
5434 832.8785 1663.7424
5435 832.8785 1663.7424
5436 832.8785 1663.7424
5437 832.8785 1663.7424
5438 832.8785 1663.7424
5439 832.8785 1663.7424
5440 832.8785 1663.7424
5441 832.8785 1663.7424
5442 832.8785 1663.7424
5443 832.8785 1663.7424
5444 832.8785 1663.7424
5445 832.8785 1663.7424
5446 832.8785 1663.7424
5447 832.8785 1663.7424
5448 832.8785 1663.7424
5449 832.8785 1663.7424
5450 832.8785 1663.7424
5451 832.8785 1663.7424
5452 832.8785 1663.7424
5453 832.8785 1663.7424
5454 832.8785 1663.7424
5455 832.8785 1663.7424
5456 832.8785 1663.7424
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