MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5401–5500 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5213 819.3723 1636.7300
5214 819.3723 1636.7300
5215 819.3723 1636.7300
5216 819.3723 1636.7300
5217 819.3723 1636.7300
5218 819.3723 1636.7300
5219 819.3723 1636.7300
5220 819.3723 1636.7300
5221 819.3723 1636.7300
5222 819.3723 1636.7300
5223 819.3723 1636.7300
5225 819.3723 1636.7300
5226 819.3723 1636.7300
5227 819.3723 1636.7300
5228 819.3723 1636.7300
5229 819.3723 1636.7300
5230 819.3723 1636.7300
5231 819.3723 1636.7300
5232 819.3723 1636.7300
5233 819.3723 1636.7300
5234 819.3723 1636.7300
5235 819.3723 1636.7300
5236 819.3723 1636.7300
5237 819.3723 1636.7300
5238 819.3723 1636.7300
5239 819.3723 1636.7300
5240 819.3723 1636.7300
5241 819.3723 1636.7300
5242 819.3723 1636.7300
5243 819.3723 1636.7300
5244 819.3723 1636.7300
5245 819.3723 1636.7300
5247 819.3723 1636.7300
5249 819.3723 1636.7300
5250 819.3723 1636.7300
5251 819.3723 1636.7300
5252 819.3723 1636.7300
5253 819.3723 1636.7300
5254 819.3723 1636.7300
5255 819.3723 1636.7300
5260 819.3723 1636.7300
5261 819.3723 1636.7300
5265 819.3723 1636.7300
5266 819.3723 1636.7300
5267 819.3723 1636.7300
5269 819.3723 1636.7300
5270 819.3723 1636.7300
5271 819.3723 1636.7300
5276 819.3723 1636.7300
5278 819.3723 1636.7300
5282 819.3723 1636.7300
5284 819.3723 1636.7300
5285 819.3723 1636.7300
5286 819.3723 1636.7300
5287 819.3723 1636.7300
5288 819.3723 1636.7300
5289 819.3723 1636.7300
5290 819.3723 1636.7300
5292 819.3723 1636.7300
5293 819.3723 1636.7300
5297 819.3723 1636.7300
5298 819.3723 1636.7300
5300 819.3723 1636.7300
5301 819.3723 1636.7300
5302 819.3723 1636.7300
5305 819.3723 1636.7300
5306 819.3723 1636.7300
5307 819.3723 1636.7300
5308 819.3723 1636.7300
5309 819.3723 1636.7300
5311 819.3723 1636.7300
5314 819.3723 1636.7300
5315 819.3723 1636.7300
5316 819.3723 1636.7300
5317 819.3723 1636.7300
5318 819.3723 1636.7300
5319 819.3723 1636.7300
5320 819.3723 1636.7300
5323 819.3723 1636.7300
5325 819.3723 1636.7300
5327 819.3723 1636.7300
5329 819.3723 1636.7300
5330 819.3723 1636.7300
5331 819.3723 1636.7300
5332 819.3723 1636.7300
5333 819.3723 1636.7300
5334 819.3723 1636.7300
5335 819.3723 1636.7300
5336 819.3723 1636.7300
5337 819.3723 1636.7300
5338 819.3723 1636.7300
5339 819.3723 1636.7300
5340 819.3723 1636.7300
5341 819.3723 1636.7300
5342 819.3723 1636.7300
5343 819.3723 1636.7300
5344 819.3723 1636.7300
5347 832.8785 1663.7424
5348 832.8785 1663.7424
5349 832.8785 1663.7424
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