MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5301–5400 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5108 805.8662 1609.7178
5110 805.8662 1609.7178
5111 805.8662 1609.7178
5112 805.8662 1609.7178
5114 805.8662 1609.7178
5115 805.8662 1609.7178
5117 805.8662 1609.7178
5118 805.8662 1609.7178
5119 805.8662 1609.7178
5120 805.8662 1609.7178
5121 805.8662 1609.7178
5123 805.8662 1609.7178
5124 805.8662 1609.7178
5125 805.8662 1609.7178
5126 805.8662 1609.7178
5127 805.8662 1609.7178
5128 805.8662 1609.7178
5129 805.8662 1609.7178
5130 805.8662 1609.7178
5131 805.8662 1609.7178
5132 805.8662 1609.7178
5133 805.8662 1609.7178
5134 805.8662 1609.7178
5135 805.8662 1609.7178
5136 805.8662 1609.7178
5137 805.8662 1609.7178
5138 805.8662 1609.7178
5139 805.8662 1609.7178
5140 805.8662 1609.7178
5141 805.8662 1609.7178
5142 805.8662 1609.7178
5143 805.8662 1609.7178
5144 805.8662 1609.7178
5145 805.8662 1609.7178
5146 805.8662 1609.7178
5147 805.8662 1609.7178
5148 805.8662 1609.7178
5149 805.8662 1609.7178
5150 805.8662 1609.7178
5151 805.8662 1609.7178
5153 805.8662 1609.7178
5154 805.8662 1609.7178
5155 805.8662 1609.7178
5156 805.8662 1609.7178
5157 805.8662 1609.7178
5158 805.8662 1609.7178
5159 805.8662 1609.7178
5160 805.8662 1609.7178
5161 805.8662 1609.7178
5162 805.8662 1609.7178
5163 805.8662 1609.7178
5164 805.8662 1609.7178
5165 805.8662 1609.7178
5166 805.8662 1609.7178
5167 805.8662 1609.7178
5168 805.8662 1609.7178
5169 805.8662 1609.7178
5170 805.8662 1609.7178
5171 805.8662 1609.7178
5172 805.8662 1609.7178
5173 805.8662 1609.7178
5174 805.8662 1609.7178
5175 805.8662 1609.7178
5176 805.8662 1609.7178
5177 805.8662 1609.7178
5178 805.8662 1609.7178
5179 805.8662 1609.7178
5180 805.8662 1609.7178
5181 805.8662 1609.7178
5182 805.8662 1609.7178
5183 805.8662 1609.7178
5184 805.8662 1609.7178
5185 819.3723 1636.7300
5186 819.3723 1636.7300
5187 819.3723 1636.7300
5188 819.3723 1636.7300
5189 819.3723 1636.7300
5190 819.3723 1636.7300
5191 819.3723 1636.7300
5192 819.3723 1636.7300
5193 819.3723 1636.7300
5194 819.3723 1636.7300
5195 819.3723 1636.7300
5196 819.3723 1636.7300
5197 819.3723 1636.7300
5198 819.3723 1636.7300
5199 819.3723 1636.7300
5200 819.3723 1636.7300
5201 819.3723 1636.7300
5202 819.3723 1636.7300
5203 819.3723 1636.7300
5204 819.3723 1636.7300
5205 819.3723 1636.7300
5206 819.3723 1636.7300
5207 819.3723 1636.7300
5208 819.3723 1636.7300
5209 819.3723 1636.7300
5210 819.3723 1636.7300
5211 819.3723 1636.7300
5212 819.3723 1636.7300
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