MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5201–5300 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5004 792.3600 1582.7054
5006 792.3600 1582.7054
5007 792.3600 1582.7054
5008 792.3600 1582.7054
5009 792.3600 1582.7054
5010 792.3600 1582.7054
5011 792.3600 1582.7054
5012 792.3600 1582.7054
5013 792.3600 1582.7054
5014 792.3600 1582.7054
5015 792.3600 1582.7054
5016 792.3600 1582.7054
5017 792.3600 1582.7054
5018 792.3600 1582.7054
5019 792.3600 1582.7054
5020 792.3600 1582.7054
5021 792.3600 1582.7054
5022 792.3600 1582.7054
5023 805.8502 1609.6858
5024 805.8502 1609.6858
5025 805.8513 1609.6880
5026 805.8662 1609.7178
5027 805.8662 1609.7178
5028 805.8662 1609.7178
5029 805.8662 1609.7178
5030 805.8662 1609.7178
5031 805.8662 1609.7178
5032 805.8662 1609.7178
5033 805.8662 1609.7178
5034 805.8662 1609.7178
5035 805.8662 1609.7178
5036 805.8662 1609.7178
5038 805.8662 1609.7178
5039 805.8662 1609.7178
5040 805.8662 1609.7178
5041 805.8662 1609.7178
5042 805.8662 1609.7178
5043 805.8662 1609.7178
5044 805.8662 1609.7178
5045 805.8662 1609.7178
5046 805.8662 1609.7178
5047 805.8662 1609.7178
5048 805.8662 1609.7178
5049 805.8662 1609.7178
5050 805.8662 1609.7178
5051 805.8662 1609.7178
5052 805.8662 1609.7178
5053 805.8662 1609.7178
5054 805.8662 1609.7178
5055 805.8662 1609.7178
5056 805.8662 1609.7178
5057 805.8662 1609.7178
5058 805.8662 1609.7178
5059 805.8662 1609.7178
5060 805.8662 1609.7178
5061 805.8662 1609.7178
5062 805.8662 1609.7178
5063 805.8662 1609.7178
5064 805.8662 1609.7178
5065 805.8662 1609.7178
5066 805.8662 1609.7178
5067 805.8662 1609.7178
5068 805.8662 1609.7178
5069 805.8662 1609.7178
5070 805.8662 1609.7178
5071 805.8662 1609.7178
5072 805.8662 1609.7178
5073 805.8662 1609.7178
5075 805.8662 1609.7178
5076 805.8662 1609.7178
5077 805.8662 1609.7178
5078 805.8662 1609.7178
5079 805.8662 1609.7178
5080 805.8662 1609.7178
5081 805.8662 1609.7178
5082 805.8662 1609.7178
5083 805.8662 1609.7178
5084 805.8662 1609.7178
5085 805.8662 1609.7178
5086 805.8662 1609.7178
5087 805.8662 1609.7178
5088 805.8662 1609.7178
5089 805.8662 1609.7178
5090 805.8662 1609.7178
5091 805.8662 1609.7178
5092 805.8662 1609.7178
5093 805.8662 1609.7178
5094 805.8662 1609.7178
5095 805.8662 1609.7178
5096 805.8662 1609.7178
5097 805.8662 1609.7178
5098 805.8662 1609.7178
5099 805.8662 1609.7178
5100 805.8662 1609.7178
5101 805.8662 1609.7178
5102 805.8662 1609.7178
5103 805.8662 1609.7178
5104 805.8662 1609.7178
5105 805.8662 1609.7178
5106 805.8662 1609.7178
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