MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5101–5200 (out of 7204)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4892 792.3507 1582.6868
4893 792.3516 1582.6886
4894 792.3530 1582.6914
4895 792.3600 1582.7054
4896 792.3600 1582.7054
4897 792.3600 1582.7054
4898 792.3600 1582.7054
4899 792.3600 1582.7054
4900 792.3600 1582.7054
4901 792.3600 1582.7054
4902 792.3600 1582.7054
4903 792.3600 1582.7054
4904 792.3600 1582.7054
4905 792.3600 1582.7054
4906 792.3600 1582.7054
4907 792.3600 1582.7054
4908 792.3600 1582.7054
4909 792.3600 1582.7054
4910 792.3600 1582.7054
4911 792.3600 1582.7054
4912 792.3600 1582.7054
4913 792.3600 1582.7054
4914 792.3600 1582.7054
4915 792.3600 1582.7054
4916 792.3600 1582.7054
4917 792.3600 1582.7054
4918 792.3600 1582.7054
4919 792.3600 1582.7054
4920 792.3600 1582.7054
4921 792.3600 1582.7054
4922 792.3600 1582.7054
4923 792.3600 1582.7054
4924 792.3600 1582.7054
4925 792.3600 1582.7054
4926 792.3600 1582.7054
4927 792.3600 1582.7054
4928 792.3600 1582.7054
4929 792.3600 1582.7054
4930 792.3600 1582.7054
4931 792.3600 1582.7054
4932 792.3600 1582.7054
4933 792.3600 1582.7054
4934 792.3600 1582.7054
4935 792.3600 1582.7054
4936 792.3600 1582.7054
4937 792.3600 1582.7054
4938 792.3600 1582.7054
4939 792.3600 1582.7054
4940 792.3600 1582.7054
4941 792.3600 1582.7054
4942 792.3600 1582.7054
4943 792.3600 1582.7054
4944 792.3600 1582.7054
4945 792.3600 1582.7054
4946 792.3600 1582.7054
4947 792.3600 1582.7054
4948 792.3600 1582.7054
4953 792.3600 1582.7054
4955 792.3600 1582.7054
4956 792.3600 1582.7054
4957 792.3600 1582.7054
4958 792.3600 1582.7054
4959 792.3600 1582.7054
4961 792.3600 1582.7054
4962 792.3600 1582.7054
4963 792.3600 1582.7054
4964 792.3600 1582.7054
4965 792.3600 1582.7054
4966 792.3600 1582.7054
4967 792.3600 1582.7054
4969 792.3600 1582.7054
4970 792.3600 1582.7054
4971 792.3600 1582.7054
4972 792.3600 1582.7054
4973 792.3600 1582.7054
4974 792.3600 1582.7054
4975 792.3600 1582.7054
4976 792.3600 1582.7054
4978 792.3600 1582.7054
4979 792.3600 1582.7054
4980 792.3600 1582.7054
4981 792.3600 1582.7054
4982 792.3600 1582.7054
4983 792.3600 1582.7054
4984 792.3600 1582.7054
4986 792.3600 1582.7054
4988 792.3600 1582.7054
4990 792.3600 1582.7054
4991 792.3600 1582.7054
4992 792.3600 1582.7054
4993 792.3600 1582.7054
4994 792.3600 1582.7054
4996 792.3600 1582.7054
4997 792.3600 1582.7054
4998 792.3600 1582.7054
4999 792.3600 1582.7054
5000 792.3600 1582.7054
5001 792.3600 1582.7054
5002 792.3600 1582.7054
5003 792.3600 1582.7054
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