MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 5001–5100 (out of 7204)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4771 778.8539 1555.6932
4772 778.8539 1555.6932
4773 778.8539 1555.6932
4774 778.8539 1555.6932
4775 778.8539 1555.6932
4777 778.8539 1555.6932
4778 778.8539 1555.6932
4779 778.8539 1555.6932
4780 778.8539 1555.6932
4782 778.8539 1555.6932
4783 778.8539 1555.6932
4785 778.8539 1555.6932
4788 778.8539 1555.6932
4790 778.8539 1555.6932
4791 778.8539 1555.6932
4792 778.8539 1555.6932
4793 778.8539 1555.6932
4794 778.8539 1555.6932
4795 778.8539 1555.6932
4796 778.8539 1555.6932
4797 778.8539 1555.6932
4798 778.8539 1555.6932
4800 778.8539 1555.6932
4801 778.8539 1555.6932
4804 778.8539 1555.6932
4805 778.8539 1555.6932
4807 778.8539 1555.6932
4808 778.8539 1555.6932
4809 778.8539 1555.6932
4810 778.8539 1555.6932
4811 778.8539 1555.6932
4812 778.8539 1555.6932
4813 778.8539 1555.6932
4814 778.8539 1555.6932
4816 778.8539 1555.6932
4817 778.8539 1555.6932
4818 778.8539 1555.6932
4819 778.8539 1555.6932
4821 778.8539 1555.6932
4823 778.8539 1555.6932
4824 778.8539 1555.6932
4825 778.8539 1555.6932
4826 778.8539 1555.6932
4827 778.8539 1555.6932
4830 778.8539 1555.6932
4831 778.8539 1555.6932
4832 778.8539 1555.6932
4833 778.8539 1555.6932
4835 778.8539 1555.6932
4837 778.8539 1555.6932
4838 778.8539 1555.6932
4839 778.8539 1555.6932
4840 778.8539 1555.6932
4843 778.8539 1555.6932
4845 778.8539 1555.6932
4847 778.8539 1555.6932
4848 778.8539 1555.6932
4849 778.8539 1555.6932
4850 778.8539 1555.6932
4851 778.8539 1555.6932
4852 778.8539 1555.6932
4853 778.8539 1555.6932
4854 778.8539 1555.6932
4855 778.8539 1555.6932
4856 778.8539 1555.6932
4857 778.8539 1555.6932
4858 778.8589 1555.7032
4859 778.8611 1555.7076
4860 778.8662 1555.7178
4861 792.3442 1582.6738
4862 792.3447 1582.6748
4863 792.3450 1582.6754
4864 792.3452 1582.6758
4865 792.3455 1582.6764
4866 792.3460 1582.6774
4867 792.3465 1582.6784
4868 792.3467 1582.6788
4869 792.3467 1582.6788
4870 792.3468 1582.6790
4871 792.3468 1582.6790
4872 792.3470 1582.6794
4873 792.3471 1582.6796
4874 792.3473 1582.6800
4875 792.3474 1582.6802
4876 792.3474 1582.6802
4877 792.3480 1582.6814
4878 792.3481 1582.6816
4879 792.3481 1582.6816
4880 792.3483 1582.6820
4881 792.3483 1582.6820
4882 792.3488 1582.6830
4883 792.3489 1582.6832
4884 792.3491 1582.6836
4885 792.3491 1582.6836
4886 792.3491 1582.6836
4887 792.3493 1582.6840
4888 792.3497 1582.6848
4889 792.3500 1582.6854
4890 792.3502 1582.6858
4891 792.3503 1582.6860
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