MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 4901–5000 (out of 7204)

Page: Previous 1 …45 46 47 48 49 50 51 52 53 54 55 … 73 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4662 765.3478 1528.6810
4663 765.3478 1528.6810
4664 765.3478 1528.6810
4665 765.3478 1528.6810
4666 765.3478 1528.6810
4667 765.3478 1528.6810
4668 765.3478 1528.6810
4669 765.3478 1528.6810
4670 765.3478 1528.6810
4671 765.3478 1528.6810
4672 765.3478 1528.6810
4673 765.3478 1528.6810
4674 765.3478 1528.6810
4675 765.3478 1528.6810
4676 765.3478 1528.6810
4677 765.3478 1528.6810
4678 765.3478 1528.6810
4679 765.3478 1528.6810
4680 765.3478 1528.6810
4681 765.3478 1528.6810
4684 765.3478 1528.6810
4685 765.3478 1528.6810
4686 765.3478 1528.6810
4687 765.3478 1528.6810
4688 765.3478 1528.6810
4689 765.3478 1528.6810
4691 765.3524 1528.6902
4692 765.3527 1528.6908
4693 765.3531 1528.6916
4695 765.3544 1528.6942
4696 765.3560 1528.6974
4697 765.3599 1528.7052
4698 765.3613 1528.7080
4699 778.8539 1555.6932
4700 778.8539 1555.6932
4701 778.8539 1555.6932
4702 778.8539 1555.6932
4703 778.8539 1555.6932
4704 778.8539 1555.6932
4705 778.8539 1555.6932
4706 778.8539 1555.6932
4707 778.8539 1555.6932
4708 778.8539 1555.6932
4709 778.8539 1555.6932
4710 778.8539 1555.6932
4711 778.8539 1555.6932
4712 778.8539 1555.6932
4713 778.8539 1555.6932
4714 778.8539 1555.6932
4715 778.8539 1555.6932
4716 778.8539 1555.6932
4717 778.8539 1555.6932
4718 778.8539 1555.6932
4719 778.8539 1555.6932
4720 778.8539 1555.6932
4721 778.8539 1555.6932
4722 778.8539 1555.6932
4723 778.8539 1555.6932
4724 778.8539 1555.6932
4725 778.8539 1555.6932
4726 778.8539 1555.6932
4727 778.8539 1555.6932
4728 778.8539 1555.6932
4729 778.8539 1555.6932
4730 778.8539 1555.6932
4731 778.8539 1555.6932
4732 778.8539 1555.6932
4733 778.8539 1555.6932
4734 778.8539 1555.6932
4735 778.8539 1555.6932
4736 778.8539 1555.6932
4737 778.8539 1555.6932
4738 778.8539 1555.6932
4739 778.8539 1555.6932
4740 778.8539 1555.6932
4741 778.8539 1555.6932
4742 778.8539 1555.6932
4743 778.8539 1555.6932
4744 778.8539 1555.6932
4745 778.8539 1555.6932
4746 778.8539 1555.6932
4747 778.8539 1555.6932
4748 778.8539 1555.6932
4749 778.8539 1555.6932
4750 778.8539 1555.6932
4751 778.8539 1555.6932
4752 778.8539 1555.6932
4753 778.8539 1555.6932
4754 778.8539 1555.6932
4755 778.8539 1555.6932
4757 778.8539 1555.6932
4758 778.8539 1555.6932
4759 778.8539 1555.6932
4760 778.8539 1555.6932
4761 778.8539 1555.6932
4762 778.8539 1555.6932
4763 778.8539 1555.6932
4766 778.8539 1555.6932
4769 778.8539 1555.6932
4770 778.8539 1555.6932
Page: Previous 1 …45 46 47 48 49 50 51 52 53 54 55 … 73 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/