MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3643 684.3109 1366.6072 1366.6299 -16.6 1 2 1.3 +1Score > 16 indicates identity SRDAFIDEMQR
6099 886.9030 1771.7914 1771.8028 -6.42 0 2 1.4 +1Score > 16 indicates identity LNDEMFEVTFWWR
6556 927.4214 1852.8282 1852.8625 -18.5 1 2 1.6 +1Score > 17 indicates identity LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
4952 792.3600 1582.7054 1582.7045 0.58 1 2 1 +1Score > 15 indicates identity CQYETLVENNRR + 2 Deamidated (NQ)
1625 522.2309 1042.4472 1042.4277 18.7 0 2 0.65 +1Score > 13 indicates identity FSLGNMDDK + Deamidated (NQ); Oxidation (M)
2175 562.7557 1123.4968 1123.4894 6.65 0 2 0.96 +1Score > 14 indicates identity NLNDTNYNR + Deamidated (NQ)
2312 576.2618 1150.5090 1150.4891 17.4 0 2 1.1 +1Score > 15 indicates identity DEAQEQQFR + Deamidated (NQ)
4117 724.8293 1447.6440 1447.6725 -19.7 1 2 0.77 +1Score > 13 indicates identity QLDSEACRELAR + Deamidated (NQ)
6435 913.9153 1825.8160 1825.8251 -4.96 0 2 1.7 +1Score > 17 indicates identity MTNNDTTLQLSSVLNR + 4 Deamidated (NQ); Oxidation (M)
6409 913.9153 1825.8160 1825.7900 14.2 0 2 1.7 +1Score > 17 indicates identity NGYNSAALNGDMNQALR + 2 Deamidated (NQ); Oxidation (M)
2656 603.2741 1204.5336 1204.5295 3.46 0 2 1.4 +1Score > 16 indicates identity HACGYELAER
755 441.2004 880.3862 880.3926 -7.26 0 2 1 +1Score > 15 indicates identity QQGEVYR + 2 Deamidated (NQ)
3926 711.3232 1420.6318 1420.6252 4.68 1 2 1.5 +1Score > 16 indicates identity ANNAINDMVRQR + 4 Deamidated (NQ); Oxidation (M)
5326 819.3723 1636.7300 1636.7403 -6.24 0 2 1.5 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
5277 819.3723 1636.7300 1636.7403 -6.24 0 2 1.5 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
6596 927.4214 1852.8282 1852.8631 -18.8 0 2 1.6 +1Score > 17 indicates identity SSIQLISSNWPYWNR + 3 Deamidated (NQ)
1030 468.2127 934.4108 934.4178 -7.46 1 2 1.2 +1Score > 15 indicates identity LDEDCKR
1853 535.7434 1069.4722 1069.4862 -13.1 0 2 0.63 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
3778 697.8171 1393.6196 1393.6078 8.49 1 2 1.5 +1Score > 16 indicates identity RNGMSGVQCVER + 2 Deamidated (NQ)
5876 873.3969 1744.7792 1744.7838 -2.63 1 2 2.2 +1Score > 18 indicates identity AREASGFGADYNAMIR + Deamidated (NQ); Oxidation (M)
6586 927.4214 1852.8282 1852.8625 -18.5 1 2 1.6 +1Score > 17 indicates identity LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
6615 927.4214 1852.8282 1852.8241 2.25 1 2 1.6 +1Score > 17 indicates identity EWYEKHGNSHQGEPR
636 427.6943 853.3740 853.3600 16.5 0 2 0.63 +1Score > 13 indicates identity MQTSDTR + Oxidation (M)
5986 873.3999 1744.7852 1744.7735 6.72 0 2 2.2 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
6262 900.4092 1798.8038 1798.7870 9.35 0 2 1.1 +1Score > 15 indicates identity TQADAWDINQGHQSAR + 2 Deamidated (NQ)
7053 967.9399 1933.8652 1933.8476 9.14 0 2 1.7 +1Score > 17 indicates identity MSTQYETQGYTINNAGR + Deamidated (NQ)
2470 589.7679 1177.5212 1177.5107 8.95 0 2 0.92 +1Score > 14 indicates identity MQQSIDPAMR + 2 Deamidated (NQ)
6381 913.9153 1825.8160 1825.8483 -17.6 0 2 1.8 +1Score > 17 indicates identity QFTLDDVSGAEFNQQK
1175 481.7188 961.4230 961.4253 -2.38 0 2 1 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
2797 616.7802 1231.5458 1231.5615 -12.7 1 2 1.1 +1Score > 15 indicates identity IREMSDNDPR
2530 589.7679 1177.5212 1177.5107 8.95 0 2 0.93 +1Score > 14 indicates identity MQQSIDPAMR + 2 Deamidated (NQ)
3202 643.7925 1285.5704 1285.5609 7.46 0 2 1.1 +1Score > 15 indicates identity DNGAEYTMTLR + Oxidation (M)
1819 535.7434 1069.4722 1069.4862 -13.1 1 2 0.64 +1Score > 13 indicates identity EAMGFSEKR + Oxidation (M)
5789 859.8907 1717.7668 1717.7907 -13.9 1 2 1.2 +1Score > 15 indicates identity SVVNNDPQGYPNRVR + 4 Deamidated (NQ)
6271 900.4092 1798.8038 1798.7953 4.76 0 2 1.1 +1Score > 15 indicates identity CLNHMFAAEIYMQR + Oxidation (M)
6883 954.4337 1906.8528 1906.8877 -18.3 1 2 1.9 +1Score > 17 indicates identity EQQLEVMTGCRTGQIR + 2 Deamidated (NQ)
700 441.1945 880.3744 880.3596 16.9 0 2 0.71 +1Score > 13 indicates identity LNSQMNR + 3 Deamidated (NQ); Oxidation (M)
3399 657.3034 1312.5922 1312.6160 -18.1 0 2 1.4 +1Score > 16 indicates identity VQGNHNPYVQR + 2 Deamidated (NQ)
1092 468.2127 934.4108 934.4290 -19.5 1 2 1.2 +1Score > 15 indicates identity LNRSCER + Deamidated (NQ)
3968 711.3232 1420.6318 1420.6503 -13.0 1 2 1.6 +1Score > 16 indicates identity AEAQIAKMEEQR + 2 Deamidated (NQ); Oxidation (M)
4046 711.3232 1420.6318 1420.6512 -13.6 1 2 1.6 +1Score > 16 indicates identity QMMGMEPKQIAR + 2 Deamidated (NQ)
5618 846.3846 1690.7546 1690.7879 -19.7 1 2 1.4 +1Score > 16 indicates identity RIAAGCGMQVQDVNR + Deamidated (NQ); Oxidation (M)
6463 913.9153 1825.8160 1825.8482 -17.6 1 2 1.8 +1Score > 17 indicates identity EDAQLLYGFNNKQER + 2 Deamidated (NQ)
1281 481.7188 961.4230 961.4215 1.59 0 2 1 +1Score > 15 indicates identity GSMTFFEK + Oxidation (M)
3111 643.7925 1285.5704 1285.5761 -4.41 0 2 1.1 +1Score > 15 indicates identity MQAYFDQLDR
3781 697.8171 1393.6196 1393.6474 -19.9 0 2 1.6 +1Score > 16 indicates identity GDGSLADFGEALSR
7046 967.9399 1933.8652 1933.9017 -18.9 1 2 1.7 +1Score > 17 indicates identity QIYEDQLSSLHNDSKR + 2 Deamidated (NQ)
4768 778.8539 1555.6932 1555.6647 18.4 0 2 1.2 +1Score > 15 indicates identity CMFIDEQNVINR + 2 Deamidated (NQ); Oxidation (M)
4694 765.3541 1528.6936 1528.7045 -7.13 0 2 1.6 +1Score > 16 indicates identity EQDHFVALDTQPK + 2 Deamidated (NQ)
6546 927.4214 1852.8282 1852.8625 -18.5 1 2 1.7 +1Score > 17 indicates identity LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
6576 927.4214 1852.8282 1852.8241 2.25 1 2 1.7 +1Score > 17 indicates identity EWYEKHGNSHQGEPR
1808 535.7434 1069.4722 1069.4862 -13.1 0 2 0.65 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
1906 535.7434 1069.4722 1069.4902 -16.8 0 2 0.66 +1Score > 13 indicates identity YQLNMWSK + Deamidated (NQ)
5465 832.8785 1663.7424 1663.7478 -3.21 0 2 1.4 +1Score > 16 indicates identity LTGDEGPEYWAAQAR + Deamidated (NQ)
6241 900.4092 1798.8038 1798.8308 -15.0 0 2 1.2 +1Score > 15 indicates identity IYDSDSILSMHHEPR
2946 630.2830 1258.5514 1258.5621 -8.43 0 2 0.66 +1Score > 13 indicates identity ISMMPGMPSHR + Oxidation (M)
3131 643.7925 1285.5704 1285.5761 -4.41 0 2 1.1 +1Score > 15 indicates identity MQAYFDQLDR
7057 967.9399 1933.8652 1933.9017 -18.9 1 2 1.8 +1Score > 17 indicates identity QDIDRWQSTSVNDVLR + 3 Deamidated (NQ)
3061 630.2864 1258.5582 1258.5387 15.5 0 2 0.89 +1Score > 14 indicates identity YDSSATLDEMK
2828 616.7802 1231.5458 1231.5615 -12.7 1 2 1.2 +1Score > 15 indicates identity IREMSDNDPR
6243 900.4092 1798.8038 1798.8308 -15.0 0 2 1.2 +1Score > 15 indicates identity IYDSDSILSMHHEPR
4844 778.8539 1555.6932 1555.7114 -11.7 0 2 1.3 +1Score > 15 indicates identity SDATADQHQLQALR + 3 Deamidated (NQ)
3799 697.8171 1393.6196 1393.6031 11.9 0 2 1.6 +1Score > 16 indicates identity TEQVNQQLMER + 3 Deamidated (NQ); Oxidation (M)
6542 927.4214 1852.8282 1852.8625 -18.5 1 2 1.7 +1Score > 17 indicates identity LNNKEMNAQLDYLNR + 2 Deamidated (NQ); Oxidation (M)
623 427.6943 853.3740 853.3851 -13.0 1 2 0.67 +1Score > 13 indicates identity MSSKEQK + Deamidated (NQ); Oxidation (M)
3344 657.2986 1312.5826 1312.5757 5.26 0 2 1.5 +1Score > 16 indicates identity EYTWANLMER + Deamidated (NQ)
4764 778.8539 1555.6932 1555.7123 -12.2 1 2 1.3 +1Score > 15 indicates identity FVENKSQLMGACR + Deamidated (NQ); Oxidation (M)
5263 819.3723 1636.7300 1636.7337 -2.26 0 2 1.7 +1Score > 16 indicates identity YMGNNGPMQLEVPR + 2 Oxidation (M)
664 441.1931 880.3716 880.3562 17.5 0 2 0.81 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
1687 522.2372 1042.4598 1042.4432 16.0 1 2 0.68 +1Score > 13 indicates identity MMMMQSRK + Deamidated (NQ)
3351 657.2986 1312.5826 1312.5830 -0.24 0 2 1.5 +1Score > 16 indicates identity NHCEILQGNAR + 2 Deamidated (NQ)
6559 927.4214 1852.8282 1852.8625 -18.5 1 2 1.7 +1Score > 17 indicates identity NQKIGSLGMDVYENER + Deamidated (NQ)
3546 670.8048 1339.5950 1339.6143 -14.4 0 2 1.6 +1Score > 16 indicates identity TGVSNTLENEFK + 2 Deamidated (NQ)
6581 927.4214 1852.8282 1852.8056 12.2 0 2 1.7 +1Score > 17 indicates identity FYQNWQPAWAPGTQR + 4 Deamidated (NQ)
4829 778.8539 1555.6932 1555.7049 -7.48 0 2 1.3 +1Score > 15 indicates identity EGHIQQQMGGINAR + 2 Deamidated (NQ); Oxidation (M)
4951 792.3600 1582.7054 1582.7110 -3.53 0 2 1.1 +1Score > 15 indicates identity ALNNELQQEVHQR + 5 Deamidated (NQ)
3665 684.3109 1366.6072 1366.6153 -5.91 1 2 1.5 +1Score > 16 indicates identity FQQQKDWETR + 2 Deamidated (NQ)
6402 913.9153 1825.8160 1825.8304 -7.88 0 2 1.9 +1Score > 17 indicates identity QMYGLNAIEHSNYLR + 2 Deamidated (NQ); Oxidation (M)
6554 927.4214 1852.8282 1852.8241 2.25 1 2 1.7 +1Score > 17 indicates identity EWYEKHGNSHQGEPR
701 441.1945 880.3744 880.3596 16.9 0 2 0.76 +1Score > 13 indicates identity LNSQMNR + 3 Deamidated (NQ); Oxidation (M)
2978 630.2844 1258.5542 1258.5621 -6.20 0 2 0.72 +1Score > 13 indicates identity ISMMPGMPSHR + Oxidation (M)
3121 643.7925 1285.5704 1285.5761 -4.41 0 2 1.1 +1Score > 15 indicates identity MQAYFDQLDR
3242 657.2872 1312.5598 1312.5605 -0.50 0 2 0.93 +1Score > 14 indicates identity MVINGYQSDQR + 3 Deamidated (NQ)
4690 765.3518 1528.6890 1528.7045 -10.1 0 2 1.3 +1Score > 15 indicates identity EQDHFVALDTQPK + 2 Deamidated (NQ)
658 441.1930 880.3714 880.3562 17.3 0 2 0.83 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
4820 778.8539 1555.6932 1555.7163 -14.8 0 2 1.3 +1Score > 15 indicates identity DMFWQLMVDLSR + Oxidation (M)
2837 616.7802 1231.5458 1231.5503 -3.61 0 2 1.2 +1Score > 15 indicates identity IGMENGTTHQK + Deamidated (NQ); Oxidation (M)
3559 670.8048 1339.5950 1339.5900 3.74 0 2 1.7 +1Score > 16 indicates identity MMLEHYQQTK + 2 Oxidation (M)
3654 684.3109 1366.6072 1366.6153 -5.91 1 2 1.5 +1Score > 16 indicates identity FQQQKDWETR + 2 Deamidated (NQ)
6684 940.9276 1879.8406 1879.8046 19.2 1 2 2.1 +1Score > 17 indicates identity VNYYLERGFNMDDSR + 2 Deamidated (NQ)
1621 522.2269 1042.4392 1042.4277 11.1 0 2 0.69 +1Score > 13 indicates identity FSLGNMDDK + Deamidated (NQ); Oxidation (M)
7067 967.9399 1933.8652 1933.8476 9.14 0 2 1.8 +1Score > 17 indicates identity MSTQYETQGYTINNAGR + Deamidated (NQ)
690 441.1942 880.3738 880.3562 20.0 0 2 0.87 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
678 441.1936 880.3726 880.3562 18.6 0 2 0.87 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
3535 670.8048 1339.5950 1339.6125 -13.0 1 2 1.7 +1Score > 16 indicates identity AANQAFMMERR + Oxidation (M)
656 441.1928 880.3710 880.3562 16.8 0 2 0.84 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
3542 670.8048 1339.5950 1339.5827 9.25 0 2 1.7 +1Score > 16 indicates identity MQQNQSGFDLR + Deamidated (NQ); Oxidation (M)
3550 670.8048 1339.5950 1339.5900 3.74 0 2 1.7 +1Score > 16 indicates identity MMLEHYQQTK + 2 Oxidation (M)
4842 778.8539 1555.6932 1555.7114 -11.7 0 2 1.3 +1Score > 15 indicates identity SDATADQHQLQALR + 3 Deamidated (NQ)
4281 738.3355 1474.6564 1474.6582 -1.22 1 2 1.4 +1Score > 15 indicates identity AECQNQQGNLRR + 2 Deamidated (NQ)
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