MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4701–4800 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4435 751.8416 1501.6686
4436 751.8416 1501.6686
4437 751.8416 1501.6686
4438 751.8416 1501.6686
4439 751.8416 1501.6686
4441 751.8416 1501.6686
4442 751.8416 1501.6686
4443 751.8416 1501.6686
4444 751.8416 1501.6686
4445 751.8416 1501.6686
4446 751.8416 1501.6686
4447 751.8416 1501.6686
4452 751.8416 1501.6686
4453 751.8416 1501.6686
4454 751.8416 1501.6686
4457 751.8416 1501.6686
4459 751.8416 1501.6686
4460 751.8416 1501.6686
4461 751.8416 1501.6686
4462 751.8416 1501.6686
4463 751.8416 1501.6686
4464 751.8416 1501.6686
4465 751.8416 1501.6686
4467 751.8416 1501.6686
4468 751.8416 1501.6686
4469 751.8416 1501.6686
4470 751.8416 1501.6686
4473 751.8416 1501.6686
4474 751.8416 1501.6686
4475 751.8416 1501.6686
4476 751.8416 1501.6686
4477 751.8416 1501.6686
4478 751.8416 1501.6686
4480 751.8416 1501.6686
4482 751.8416 1501.6686
4483 751.8416 1501.6686
4484 751.8416 1501.6686
4485 751.8416 1501.6686
4486 751.8416 1501.6686
4487 751.8416 1501.6686
4488 751.8416 1501.6686
4489 751.8416 1501.6686
4490 751.8416 1501.6686
4491 751.8416 1501.6686
4495 751.8416 1501.6686
4496 751.8416 1501.6686
4497 751.8416 1501.6686
4498 751.8416 1501.6686
4499 751.8416 1501.6686
4501 751.8416 1501.6686
4502 751.8416 1501.6686
4504 751.8416 1501.6686
4506 751.8416 1501.6686
4507 751.8416 1501.6686
4508 751.8416 1501.6686
4509 751.8416 1501.6686
4510 751.8416 1501.6686
4511 751.8416 1501.6686
4512 751.8416 1501.6686
4514 751.8416 1501.6686
4515 751.8416 1501.6686
4516 751.8416 1501.6686
4517 751.8416 1501.6686
4518 751.8416 1501.6686
4519 751.8416 1501.6686
4520 751.8416 1501.6686
4521 751.8416 1501.6686
4522 751.8416 1501.6686
4523 751.8416 1501.6686
4524 751.8416 1501.6686
4525 751.8416 1501.6686
4526 751.8416 1501.6686
4527 751.8416 1501.6686
4528 751.8416 1501.6686
4529 751.8416 1501.6686
4530 751.8416 1501.6686
4531 751.8416 1501.6686
4532 751.8416 1501.6686
4533 751.8416 1501.6686
4534 751.8416 1501.6686
4536 751.8481 1501.6816
4537 765.3334 1528.6522
4538 765.3336 1528.6526
4539 765.3342 1528.6538
4540 765.3347 1528.6548
4541 765.3361 1528.6576
4542 765.3364 1528.6582
4543 765.3372 1528.6598
4544 765.3425 1528.6704
4545 765.3478 1528.6810
4546 765.3478 1528.6810
4547 765.3478 1528.6810
4548 765.3478 1528.6810
4549 765.3478 1528.6810
4550 765.3478 1528.6810
4551 765.3478 1528.6810
4552 765.3478 1528.6810
4553 765.3478 1528.6810
4554 765.3478 1528.6810
4555 765.3478 1528.6810
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