MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4601–4700 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4327 738.3355 1474.6564
4328 738.3355 1474.6564
4329 738.3355 1474.6564
4330 738.3355 1474.6564
4331 738.3355 1474.6564
4332 738.3355 1474.6564
4333 738.3355 1474.6564
4334 738.3355 1474.6564
4335 738.3355 1474.6564
4336 738.3355 1474.6564
4337 738.3355 1474.6564
4338 738.3355 1474.6564
4339 738.3355 1474.6564
4340 738.3355 1474.6564
4341 738.3355 1474.6564
4342 738.3355 1474.6564
4343 738.3355 1474.6564
4344 738.3355 1474.6564
4346 738.3355 1474.6564
4347 738.3355 1474.6564
4348 738.3355 1474.6564
4349 738.3355 1474.6564
4350 738.3355 1474.6564
4351 738.3355 1474.6564
4353 738.3355 1474.6564
4354 738.3355 1474.6564
4355 738.3355 1474.6564
4356 738.3355 1474.6564
4357 738.3355 1474.6564
4358 738.3355 1474.6564
4359 738.3355 1474.6564
4360 738.3355 1474.6564
4361 738.3355 1474.6564
4363 738.3355 1474.6564
4364 738.3355 1474.6564
4365 738.3355 1474.6564
4366 738.3355 1474.6564
4367 738.3355 1474.6564
4368 738.3355 1474.6564
4369 738.3355 1474.6564
4370 738.3355 1474.6564
4371 738.3355 1474.6564
4372 738.3355 1474.6564
4373 738.3355 1474.6564
4374 738.3355 1474.6564
4375 751.8416 1501.6686
4376 751.8416 1501.6686
4377 751.8416 1501.6686
4378 751.8416 1501.6686
4379 751.8416 1501.6686
4380 751.8416 1501.6686
4381 751.8416 1501.6686
4382 751.8416 1501.6686
4383 751.8416 1501.6686
4384 751.8416 1501.6686
4385 751.8416 1501.6686
4386 751.8416 1501.6686
4387 751.8416 1501.6686
4388 751.8416 1501.6686
4389 751.8416 1501.6686
4390 751.8416 1501.6686
4391 751.8416 1501.6686
4392 751.8416 1501.6686
4393 751.8416 1501.6686
4394 751.8416 1501.6686
4395 751.8416 1501.6686
4396 751.8416 1501.6686
4397 751.8416 1501.6686
4398 751.8416 1501.6686
4399 751.8416 1501.6686
4400 751.8416 1501.6686
4402 751.8416 1501.6686
4405 751.8416 1501.6686
4406 751.8416 1501.6686
4407 751.8416 1501.6686
4408 751.8416 1501.6686
4409 751.8416 1501.6686
4410 751.8416 1501.6686
4411 751.8416 1501.6686
4412 751.8416 1501.6686
4413 751.8416 1501.6686
4414 751.8416 1501.6686
4415 751.8416 1501.6686
4416 751.8416 1501.6686
4417 751.8416 1501.6686
4418 751.8416 1501.6686
4419 751.8416 1501.6686
4420 751.8416 1501.6686
4421 751.8416 1501.6686
4422 751.8416 1501.6686
4424 751.8416 1501.6686
4425 751.8416 1501.6686
4426 751.8416 1501.6686
4427 751.8416 1501.6686
4428 751.8416 1501.6686
4429 751.8416 1501.6686
4430 751.8416 1501.6686
4431 751.8416 1501.6686
4433 751.8416 1501.6686
4434 751.8416 1501.6686
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