MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4501–4600 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4202 724.8293 1447.6440
4203 724.8293 1447.6440
4204 724.8293 1447.6440
4205 724.8293 1447.6440
4207 724.8293 1447.6440
4208 724.8293 1447.6440
4209 724.8293 1447.6440
4210 724.8293 1447.6440
4211 724.8293 1447.6440
4212 724.8293 1447.6440
4213 738.3218 1474.6290
4214 738.3348 1474.6550
4215 738.3355 1474.6564
4216 738.3355 1474.6564
4217 738.3355 1474.6564
4218 738.3355 1474.6564
4219 738.3355 1474.6564
4220 738.3355 1474.6564
4221 738.3355 1474.6564
4222 738.3355 1474.6564
4223 738.3355 1474.6564
4224 738.3355 1474.6564
4225 738.3355 1474.6564
4226 738.3355 1474.6564
4227 738.3355 1474.6564
4228 738.3355 1474.6564
4229 738.3355 1474.6564
4230 738.3355 1474.6564
4231 738.3355 1474.6564
4232 738.3355 1474.6564
4233 738.3355 1474.6564
4234 738.3355 1474.6564
4235 738.3355 1474.6564
4236 738.3355 1474.6564
4237 738.3355 1474.6564
4238 738.3355 1474.6564
4239 738.3355 1474.6564
4240 738.3355 1474.6564
4241 738.3355 1474.6564
4242 738.3355 1474.6564
4243 738.3355 1474.6564
4244 738.3355 1474.6564
4245 738.3355 1474.6564
4246 738.3355 1474.6564
4247 738.3355 1474.6564
4248 738.3355 1474.6564
4249 738.3355 1474.6564
4250 738.3355 1474.6564
4251 738.3355 1474.6564
4252 738.3355 1474.6564
4253 738.3355 1474.6564
4254 738.3355 1474.6564
4255 738.3355 1474.6564
4256 738.3355 1474.6564
4257 738.3355 1474.6564
4258 738.3355 1474.6564
4259 738.3355 1474.6564
4260 738.3355 1474.6564
4262 738.3355 1474.6564
4263 738.3355 1474.6564
4265 738.3355 1474.6564
4268 738.3355 1474.6564
4270 738.3355 1474.6564
4271 738.3355 1474.6564
4272 738.3355 1474.6564
4273 738.3355 1474.6564
4276 738.3355 1474.6564
4277 738.3355 1474.6564
4278 738.3355 1474.6564
4283 738.3355 1474.6564
4284 738.3355 1474.6564
4285 738.3355 1474.6564
4287 738.3355 1474.6564
4288 738.3355 1474.6564
4289 738.3355 1474.6564
4290 738.3355 1474.6564
4291 738.3355 1474.6564
4292 738.3355 1474.6564
4294 738.3355 1474.6564
4296 738.3355 1474.6564
4297 738.3355 1474.6564
4298 738.3355 1474.6564
4299 738.3355 1474.6564
4300 738.3355 1474.6564
4301 738.3355 1474.6564
4302 738.3355 1474.6564
4303 738.3355 1474.6564
4304 738.3355 1474.6564
4306 738.3355 1474.6564
4307 738.3355 1474.6564
4310 738.3355 1474.6564
4312 738.3355 1474.6564
4313 738.3355 1474.6564
4317 738.3355 1474.6564
4319 738.3355 1474.6564
4320 738.3355 1474.6564
4321 738.3355 1474.6564
4323 738.3355 1474.6564
4325 738.3355 1474.6564
4326 738.3355 1474.6564
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