MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4401–4500 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4083 724.8293 1447.6440
4085 724.8293 1447.6440
4086 724.8293 1447.6440
4087 724.8293 1447.6440
4088 724.8293 1447.6440
4089 724.8293 1447.6440
4091 724.8293 1447.6440
4092 724.8293 1447.6440
4093 724.8293 1447.6440
4094 724.8293 1447.6440
4095 724.8293 1447.6440
4097 724.8293 1447.6440
4098 724.8293 1447.6440
4100 724.8293 1447.6440
4101 724.8293 1447.6440
4102 724.8293 1447.6440
4104 724.8293 1447.6440
4107 724.8293 1447.6440
4108 724.8293 1447.6440
4110 724.8293 1447.6440
4112 724.8293 1447.6440
4114 724.8293 1447.6440
4116 724.8293 1447.6440
4118 724.8293 1447.6440
4120 724.8293 1447.6440
4121 724.8293 1447.6440
4122 724.8293 1447.6440
4123 724.8293 1447.6440
4124 724.8293 1447.6440
4125 724.8293 1447.6440
4126 724.8293 1447.6440
4127 724.8293 1447.6440
4128 724.8293 1447.6440
4129 724.8293 1447.6440
4130 724.8293 1447.6440
4131 724.8293 1447.6440
4132 724.8293 1447.6440
4133 724.8293 1447.6440
4134 724.8293 1447.6440
4135 724.8293 1447.6440
4136 724.8293 1447.6440
4137 724.8293 1447.6440
4138 724.8293 1447.6440
4139 724.8293 1447.6440
4143 724.8293 1447.6440
4144 724.8293 1447.6440
4145 724.8293 1447.6440
4146 724.8293 1447.6440
4147 724.8293 1447.6440
4148 724.8293 1447.6440
4149 724.8293 1447.6440
4150 724.8293 1447.6440
4151 724.8293 1447.6440
4152 724.8293 1447.6440
4153 724.8293 1447.6440
4154 724.8293 1447.6440
4155 724.8293 1447.6440
4156 724.8293 1447.6440
4157 724.8293 1447.6440
4158 724.8293 1447.6440
4159 724.8293 1447.6440
4160 724.8293 1447.6440
4161 724.8293 1447.6440
4162 724.8293 1447.6440
4163 724.8293 1447.6440
4164 724.8293 1447.6440
4165 724.8293 1447.6440
4166 724.8293 1447.6440
4167 724.8293 1447.6440
4168 724.8293 1447.6440
4169 724.8293 1447.6440
4170 724.8293 1447.6440
4171 724.8293 1447.6440
4172 724.8293 1447.6440
4173 724.8293 1447.6440
4175 724.8293 1447.6440
4176 724.8293 1447.6440
4177 724.8293 1447.6440
4178 724.8293 1447.6440
4179 724.8293 1447.6440
4180 724.8293 1447.6440
4181 724.8293 1447.6440
4182 724.8293 1447.6440
4183 724.8293 1447.6440
4184 724.8293 1447.6440
4185 724.8293 1447.6440
4186 724.8293 1447.6440
4187 724.8293 1447.6440
4188 724.8293 1447.6440
4189 724.8293 1447.6440
4190 724.8293 1447.6440
4191 724.8293 1447.6440
4193 724.8293 1447.6440
4194 724.8293 1447.6440
4195 724.8293 1447.6440
4197 724.8293 1447.6440
4198 724.8293 1447.6440
4199 724.8293 1447.6440
4200 724.8293 1447.6440
4201 724.8293 1447.6440
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