MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4301–4400 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3967 711.3232 1420.6318
3970 711.3232 1420.6318
3971 711.3232 1420.6318
3972 711.3232 1420.6318
3975 711.3232 1420.6318
3976 711.3232 1420.6318
3977 711.3232 1420.6318
3978 711.3232 1420.6318
3979 711.3232 1420.6318
3981 711.3232 1420.6318
3982 711.3232 1420.6318
3983 711.3232 1420.6318
3985 711.3232 1420.6318
3986 711.3232 1420.6318
3987 711.3232 1420.6318
3988 711.3232 1420.6318
3989 711.3232 1420.6318
3992 711.3232 1420.6318
3993 711.3232 1420.6318
3994 711.3232 1420.6318
3995 711.3232 1420.6318
3996 711.3232 1420.6318
3997 711.3232 1420.6318
3998 711.3232 1420.6318
3999 711.3232 1420.6318
4000 711.3232 1420.6318
4001 711.3232 1420.6318
4002 711.3232 1420.6318
4003 711.3232 1420.6318
4004 711.3232 1420.6318
4005 711.3232 1420.6318
4006 711.3232 1420.6318
4007 711.3232 1420.6318
4008 711.3232 1420.6318
4009 711.3232 1420.6318
4010 711.3232 1420.6318
4011 711.3232 1420.6318
4012 711.3232 1420.6318
4013 711.3232 1420.6318
4014 711.3232 1420.6318
4015 711.3232 1420.6318
4016 711.3232 1420.6318
4017 711.3232 1420.6318
4019 711.3232 1420.6318
4020 711.3232 1420.6318
4021 711.3232 1420.6318
4022 711.3232 1420.6318
4023 711.3232 1420.6318
4024 711.3232 1420.6318
4025 711.3232 1420.6318
4026 711.3232 1420.6318
4027 711.3232 1420.6318
4028 711.3232 1420.6318
4029 711.3232 1420.6318
4030 711.3232 1420.6318
4031 711.3232 1420.6318
4032 711.3232 1420.6318
4033 711.3232 1420.6318
4034 711.3232 1420.6318
4035 711.3232 1420.6318
4036 711.3232 1420.6318
4038 711.3232 1420.6318
4039 711.3232 1420.6318
4041 711.3232 1420.6318
4043 711.3232 1420.6318
4044 711.3232 1420.6318
4045 711.3232 1420.6318
4047 711.3232 1420.6318
4048 711.3232 1420.6318
4049 711.3232 1420.6318
4051 724.8293 1447.6440
4052 724.8293 1447.6440
4053 724.8293 1447.6440
4054 724.8293 1447.6440
4055 724.8293 1447.6440
4056 724.8293 1447.6440
4057 724.8293 1447.6440
4058 724.8293 1447.6440
4059 724.8293 1447.6440
4060 724.8293 1447.6440
4061 724.8293 1447.6440
4062 724.8293 1447.6440
4063 724.8293 1447.6440
4064 724.8293 1447.6440
4065 724.8293 1447.6440
4066 724.8293 1447.6440
4067 724.8293 1447.6440
4068 724.8293 1447.6440
4070 724.8293 1447.6440
4071 724.8293 1447.6440
4072 724.8293 1447.6440
4073 724.8293 1447.6440
4075 724.8293 1447.6440
4076 724.8293 1447.6440
4077 724.8293 1447.6440
4078 724.8293 1447.6440
4079 724.8293 1447.6440
4080 724.8293 1447.6440
4081 724.8293 1447.6440
4082 724.8293 1447.6440
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