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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4201–4300 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3861 697.8171 1393.6196
3862 697.8171 1393.6196
3863 697.8171 1393.6196
3864 697.8171 1393.6196
3865 697.8171 1393.6196
3866 697.8171 1393.6196
3867 697.8171 1393.6196
3868 697.8171 1393.6196
3869 697.8171 1393.6196
3870 697.8171 1393.6196
3871 697.8171 1393.6196
3872 697.8171 1393.6196
3873 697.8171 1393.6196
3874 697.8171 1393.6196
3875 697.8171 1393.6196
3876 697.8171 1393.6196
3877 697.8171 1393.6196
3878 697.8171 1393.6196
3879 697.8171 1393.6196
3880 697.8171 1393.6196
3881 697.8290 1393.6434
3882 697.8291 1393.6436
3883 697.8291 1393.6436
3884 697.8292 1393.6438
3885 697.8293 1393.6440
3886 697.8295 1393.6444
3887 697.8297 1393.6448
3888 697.8304 1393.6462
3889 711.3099 1420.6052
3890 711.3107 1420.6068
3891 711.3135 1420.6124
3892 711.3160 1420.6174
3893 711.3204 1420.6262
3894 711.3232 1420.6318
3895 711.3232 1420.6318
3896 711.3232 1420.6318
3897 711.3232 1420.6318
3898 711.3232 1420.6318
3899 711.3232 1420.6318
3900 711.3232 1420.6318
3901 711.3232 1420.6318
3902 711.3232 1420.6318
3903 711.3232 1420.6318
3904 711.3232 1420.6318
3905 711.3232 1420.6318
3906 711.3232 1420.6318
3907 711.3232 1420.6318
3908 711.3232 1420.6318
3909 711.3232 1420.6318
3910 711.3232 1420.6318
3911 711.3232 1420.6318
3912 711.3232 1420.6318
3913 711.3232 1420.6318
3914 711.3232 1420.6318
3915 711.3232 1420.6318
3916 711.3232 1420.6318
3917 711.3232 1420.6318
3918 711.3232 1420.6318
3919 711.3232 1420.6318
3920 711.3232 1420.6318
3921 711.3232 1420.6318
3922 711.3232 1420.6318
3923 711.3232 1420.6318
3924 711.3232 1420.6318
3925 711.3232 1420.6318
3927 711.3232 1420.6318
3929 711.3232 1420.6318
3931 711.3232 1420.6318
3932 711.3232 1420.6318
3933 711.3232 1420.6318
3935 711.3232 1420.6318
3936 711.3232 1420.6318
3937 711.3232 1420.6318
3938 711.3232 1420.6318
3939 711.3232 1420.6318
3940 711.3232 1420.6318
3941 711.3232 1420.6318
3942 711.3232 1420.6318
3943 711.3232 1420.6318
3944 711.3232 1420.6318
3945 711.3232 1420.6318
3946 711.3232 1420.6318
3947 711.3232 1420.6318
3948 711.3232 1420.6318
3949 711.3232 1420.6318
3950 711.3232 1420.6318
3951 711.3232 1420.6318
3952 711.3232 1420.6318
3953 711.3232 1420.6318
3954 711.3232 1420.6318
3955 711.3232 1420.6318
3956 711.3232 1420.6318
3957 711.3232 1420.6318
3958 711.3232 1420.6318
3959 711.3232 1420.6318
3960 711.3232 1420.6318
3961 711.3232 1420.6318
3963 711.3232 1420.6318
3964 711.3232 1420.6318
3965 711.3232 1420.6318
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