MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3744 697.8171 1393.6196
3745 697.8171 1393.6196
3746 697.8171 1393.6196
3747 697.8171 1393.6196
3748 697.8171 1393.6196
3749 697.8171 1393.6196
3750 697.8171 1393.6196
3751 697.8171 1393.6196
3752 697.8171 1393.6196
3753 697.8171 1393.6196
3754 697.8171 1393.6196
3755 697.8171 1393.6196
3756 697.8171 1393.6196
3757 697.8171 1393.6196
3758 697.8171 1393.6196
3759 697.8171 1393.6196
3760 697.8171 1393.6196
3761 697.8171 1393.6196
3762 697.8171 1393.6196
3763 697.8171 1393.6196
3764 697.8171 1393.6196
3765 697.8171 1393.6196
3767 697.8171 1393.6196
3768 697.8171 1393.6196
3769 697.8171 1393.6196
3770 697.8171 1393.6196
3771 697.8171 1393.6196
3772 697.8171 1393.6196
3774 697.8171 1393.6196
3775 697.8171 1393.6196
3776 697.8171 1393.6196
3777 697.8171 1393.6196
3779 697.8171 1393.6196
3780 697.8171 1393.6196
3782 697.8171 1393.6196
3783 697.8171 1393.6196
3784 697.8171 1393.6196
3785 697.8171 1393.6196
3786 697.8171 1393.6196
3787 697.8171 1393.6196
3788 697.8171 1393.6196
3789 697.8171 1393.6196
3790 697.8171 1393.6196
3792 697.8171 1393.6196
3793 697.8171 1393.6196
3794 697.8171 1393.6196
3797 697.8171 1393.6196
3798 697.8171 1393.6196
3800 697.8171 1393.6196
3801 697.8171 1393.6196
3802 697.8171 1393.6196
3803 697.8171 1393.6196
3804 697.8171 1393.6196
3807 697.8171 1393.6196
3808 697.8171 1393.6196
3809 697.8171 1393.6196
3810 697.8171 1393.6196
3811 697.8171 1393.6196
3812 697.8171 1393.6196
3813 697.8171 1393.6196
3815 697.8171 1393.6196
3816 697.8171 1393.6196
3817 697.8171 1393.6196
3818 697.8171 1393.6196
3819 697.8171 1393.6196
3820 697.8171 1393.6196
3821 697.8171 1393.6196
3822 697.8171 1393.6196
3823 697.8171 1393.6196
3824 697.8171 1393.6196
3825 697.8171 1393.6196
3826 697.8171 1393.6196
3828 697.8171 1393.6196
3829 697.8171 1393.6196
3830 697.8171 1393.6196
3831 697.8171 1393.6196
3832 697.8171 1393.6196
3833 697.8171 1393.6196
3835 697.8171 1393.6196
3836 697.8171 1393.6196
3837 697.8171 1393.6196
3838 697.8171 1393.6196
3839 697.8171 1393.6196
3840 697.8171 1393.6196
3841 697.8171 1393.6196
3842 697.8171 1393.6196
3843 697.8171 1393.6196
3844 697.8171 1393.6196
3845 697.8171 1393.6196
3846 697.8171 1393.6196
3847 697.8171 1393.6196
3848 697.8171 1393.6196
3849 697.8171 1393.6196
3850 697.8171 1393.6196
3851 697.8171 1393.6196
3853 697.8171 1393.6196
3854 697.8171 1393.6196
3855 697.8171 1393.6196
3856 697.8171 1393.6196
3857 697.8171 1393.6196
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