MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3619 684.3109 1366.6072
3620 684.3109 1366.6072
3622 684.3109 1366.6072
3623 684.3109 1366.6072
3624 684.3109 1366.6072
3625 684.3109 1366.6072
3626 684.3109 1366.6072
3627 684.3109 1366.6072
3628 684.3109 1366.6072
3629 684.3109 1366.6072
3630 684.3109 1366.6072
3634 684.3109 1366.6072
3635 684.3109 1366.6072
3636 684.3109 1366.6072
3637 684.3109 1366.6072
3639 684.3109 1366.6072
3644 684.3109 1366.6072
3645 684.3109 1366.6072
3646 684.3109 1366.6072
3647 684.3109 1366.6072
3648 684.3109 1366.6072
3649 684.3109 1366.6072
3651 684.3109 1366.6072
3652 684.3109 1366.6072
3653 684.3109 1366.6072
3656 684.3109 1366.6072
3657 684.3109 1366.6072
3658 684.3109 1366.6072
3660 684.3109 1366.6072
3661 684.3109 1366.6072
3662 684.3109 1366.6072
3663 684.3109 1366.6072
3664 684.3109 1366.6072
3666 684.3109 1366.6072
3667 684.3109 1366.6072
3668 684.3109 1366.6072
3669 684.3109 1366.6072
3670 684.3109 1366.6072
3671 684.3109 1366.6072
3672 684.3109 1366.6072
3673 684.3109 1366.6072
3675 684.3109 1366.6072
3676 684.3109 1366.6072
3678 684.3109 1366.6072
3680 684.3109 1366.6072
3681 684.3109 1366.6072
3682 684.3109 1366.6072
3683 684.3109 1366.6072
3684 684.3109 1366.6072
3685 684.3109 1366.6072
3686 684.3109 1366.6072
3687 684.3109 1366.6072
3688 684.3109 1366.6072
3689 684.3109 1366.6072
3690 684.3109 1366.6072
3691 684.3109 1366.6072
3692 684.3109 1366.6072
3693 684.3109 1366.6072
3694 684.3109 1366.6072
3695 684.3109 1366.6072
3696 684.3109 1366.6072
3697 684.3109 1366.6072
3698 684.3109 1366.6072
3699 684.3109 1366.6072
3700 684.3109 1366.6072
3701 684.3109 1366.6072
3702 684.3109 1366.6072
3704 684.3109 1366.6072
3706 684.3109 1366.6072
3707 684.3109 1366.6072
3709 684.3109 1366.6072
3710 684.3109 1366.6072
3711 684.3109 1366.6072
3712 684.3109 1366.6072
3713 684.3109 1366.6072
3717 684.3109 1366.6072
3718 684.3109 1366.6072
3719 684.3109 1366.6072
3720 684.3109 1366.6072
3723 684.3109 1366.6072
3724 684.3162 1366.6178
3725 684.3239 1366.6332
3726 684.3243 1366.6340
3727 697.8171 1393.6196
3728 697.8171 1393.6196
3729 697.8171 1393.6196
3730 697.8171 1393.6196
3731 697.8171 1393.6196
3732 697.8171 1393.6196
3733 697.8171 1393.6196
3734 697.8171 1393.6196
3735 697.8171 1393.6196
3736 697.8171 1393.6196
3737 697.8171 1393.6196
3738 697.8171 1393.6196
3739 697.8171 1393.6196
3740 697.8171 1393.6196
3741 697.8171 1393.6196
3742 697.8171 1393.6196
3743 697.8171 1393.6196
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