MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3509 670.8048 1339.5950
3510 670.8048 1339.5950
3511 670.8048 1339.5950
3512 670.8048 1339.5950
3513 670.8048 1339.5950
3514 670.8048 1339.5950
3515 670.8048 1339.5950
3516 670.8048 1339.5950
3517 670.8048 1339.5950
3518 670.8048 1339.5950
3519 670.8048 1339.5950
3520 670.8048 1339.5950
3521 670.8048 1339.5950
3522 670.8048 1339.5950
3523 670.8048 1339.5950
3524 670.8048 1339.5950
3525 670.8048 1339.5950
3526 670.8048 1339.5950
3527 670.8048 1339.5950
3528 670.8048 1339.5950
3529 670.8048 1339.5950
3530 670.8048 1339.5950
3531 670.8048 1339.5950
3532 670.8048 1339.5950
3533 670.8048 1339.5950
3534 670.8048 1339.5950
3536 670.8048 1339.5950
3537 670.8048 1339.5950
3538 670.8048 1339.5950
3539 670.8048 1339.5950
3540 670.8048 1339.5950
3541 670.8048 1339.5950
3543 670.8048 1339.5950
3544 670.8048 1339.5950
3545 670.8048 1339.5950
3547 670.8048 1339.5950
3548 670.8048 1339.5950
3549 670.8048 1339.5950
3551 670.8048 1339.5950
3552 670.8048 1339.5950
3555 670.8048 1339.5950
3556 670.8048 1339.5950
3557 670.8048 1339.5950
3558 670.8048 1339.5950
3560 670.8048 1339.5950
3561 670.8048 1339.5950
3562 670.8048 1339.5950
3563 670.8048 1339.5950
3565 684.2974 1366.5802
3566 684.2976 1366.5806
3567 684.3026 1366.5906
3568 684.3032 1366.5918
3570 684.3035 1366.5924
3571 684.3043 1366.5940
3572 684.3045 1366.5944
3573 684.3054 1366.5962
3574 684.3058 1366.5970
3575 684.3059 1366.5972
3576 684.3063 1366.5980
3577 684.3066 1366.5986
3578 684.3066 1366.5986
3579 684.3072 1366.5998
3580 684.3076 1366.6006
3581 684.3109 1366.6072
3582 684.3109 1366.6072
3583 684.3109 1366.6072
3584 684.3109 1366.6072
3585 684.3109 1366.6072
3586 684.3109 1366.6072
3587 684.3109 1366.6072
3588 684.3109 1366.6072
3589 684.3109 1366.6072
3590 684.3109 1366.6072
3591 684.3109 1366.6072
3592 684.3109 1366.6072
3593 684.3109 1366.6072
3594 684.3109 1366.6072
3595 684.3109 1366.6072
3596 684.3109 1366.6072
3597 684.3109 1366.6072
3598 684.3109 1366.6072
3599 684.3109 1366.6072
3600 684.3109 1366.6072
3601 684.3109 1366.6072
3602 684.3109 1366.6072
3603 684.3109 1366.6072
3604 684.3109 1366.6072
3605 684.3109 1366.6072
3606 684.3109 1366.6072
3607 684.3109 1366.6072
3608 684.3109 1366.6072
3609 684.3109 1366.6072
3610 684.3109 1366.6072
3611 684.3109 1366.6072
3612 684.3109 1366.6072
3614 684.3109 1366.6072
3615 684.3109 1366.6072
3616 684.3109 1366.6072
3617 684.3109 1366.6072
3618 684.3109 1366.6072
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