MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
5913 873.3969 1744.7792 1744.7735 3.28 0 3 1.8 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
3703 684.3109 1366.6072 1366.5823 18.3 1 3 1.1 +1Score > 16 indicates identity MNTRWQQAGQK + 4 Deamidated (NQ); Oxidation (M)
6244 900.4092 1798.8038 1798.8308 -15.0 0 3 0.89 +1Score > 15 indicates identity IYDSDSILSMHHEPR
6849 954.4337 1906.8528 1906.8690 -8.48 1 3 1.5 +1Score > 17 indicates identity LSAEDNARVNVAMNQTR + 3 Deamidated (NQ); Oxidation (M)
4985 792.3600 1582.7054 1582.6875 11.4 0 3 0.84 +1Score > 15 indicates identity VYFCDPQSPWQR + Deamidated (NQ)
5321 819.3723 1636.7300 1636.7371 -4.31 1 3 1.3 +1Score > 16 indicates identity CVELVREMMGEQR + Deamidated (NQ)
4115 724.8293 1447.6440 1447.6725 -19.7 1 3 0.64 +1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
5279 819.3723 1636.7300 1636.7119 11.1 1 3 1.3 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
3398 657.3029 1312.5912 1312.5686 17.3 1 3 1.2 +1Score > 16 indicates identity IMDRMTMNGGR + 2 Oxidation (M)
4481 751.8416 1501.6686 1501.6620 4.44 1 3 1.4 +1Score > 17 indicates identity FDDRGDLSFMQR + Oxidation (M)
1249 481.7188 961.4230 961.4253 -2.38 0 3 0.83 +1Score > 15 indicates identity SGNDLHYR + Deamidated (NQ)
4987 792.3600 1582.7054 1582.6875 11.4 0 3 0.86 +1Score > 15 indicates identity VYFCDPQSPWQR + Deamidated (NQ)
5736 859.8907 1717.7668 1717.7875 -12.0 1 3 0.96 +1Score > 15 indicates identity AEDMAAANAMASHLRK + 2 Oxidation (M)
5262 819.3723 1636.7300 1636.7119 11.1 1 3 1.3 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
6144 886.9030 1771.7914 1771.7968 -3.04 0 3 1.2 +1Score > 16 indicates identity SQSTVGAGDSMVGAMTLK + Deamidated (NQ); 2 Oxidation (M)
2384 576.2618 1150.5090 1150.5117 -2.32 0 3 0.97 +1Score > 15 indicates identity YMTWDNPPK
4995 792.3600 1582.7054 1582.6875 11.4 0 3 0.87 +1Score > 15 indicates identity VYFCDPQSPWQR + Deamidated (NQ)
6767 940.9276 1879.8406 1879.8258 7.90 0 3 1.6 +1Score > 17 indicates identity MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M)
5603 846.3846 1690.7546 1690.7484 3.72 0 3 1.1 +1Score > 16 indicates identity QYLMQFCPDAVFR + Deamidated (NQ); Oxidation (M)
7082 967.9399 1933.8652 1933.8298 18.3 1 3 1.4 +1Score > 17 indicates identity QSMGFDTNMEGGFLARR + 2 Deamidated (NQ); Oxidation (M)
692 441.1942 880.3738 880.3562 20.0 0 3 0.67 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
6915 954.4337 1906.8528 1906.8877 -18.3 1 3 1.5 +1Score > 17 indicates identity EQQLEVMTGCRTGQIR + 2 Deamidated (NQ)
3324 657.2986 1312.5826 1312.6081 -19.4 1 3 1.2 +1Score > 16 indicates identity AGGTIMFSNNKR + 2 Deamidated (NQ); Oxidation (M)
5258 819.3723 1636.7300 1636.7119 11.1 1 3 1.3 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
3974 711.3232 1420.6318 1420.6187 9.25 1 3 1.3 +1Score > 16 indicates identity MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M)
4037 711.3232 1420.6318 1420.6187 9.25 1 3 1.3 +1Score > 16 indicates identity MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M)
5656 846.3858 1690.7570 1690.7475 5.67 0 3 1.2 +1Score > 16 indicates identity ESSVPFSYYDNQQK
6771 940.9276 1879.8406 1879.8469 -3.35 0 3 1.6 +1Score > 17 indicates identity NEVVADGNDSVTMTATVR + 2 Deamidated (NQ)
914 454.7066 907.3986 907.3957 3.29 0 3 0.73 +1Score > 14 indicates identity ELMEGNAK + Deamidated (NQ); Oxidation (M)
3145 643.7925 1285.5704 1285.5761 -4.41 0 3 0.89 +1Score > 15 indicates identity MQAYFDQLDR
4615 765.3478 1528.6810 1528.6576 15.3 0 3 0.87 +1Score > 15 indicates identity HQEMGQPNVSTER + Deamidated (NQ); Oxidation (M)
6525 927.4214 1852.8282 1852.8326 -2.36 1 3 1.4 +1Score > 17 indicates identity TLNSNQFDNEDAIKDK + 2 Deamidated (NQ)
4106 724.8293 1447.6440 1447.6579 -9.59 0 3 0.68 +1Score > 13 indicates identity TLWVSDDAEEQR
6857 954.4337 1906.8528 1906.8877 -18.3 1 3 1.6 +1Score > 17 indicates identity EQQLEVMTGCRTGQIR + 2 Deamidated (NQ)
2467 589.7679 1177.5212 1177.5363 -12.8 1 3 0.79 +1Score > 14 indicates identity EGENPYANKR + Deamidated (NQ)
1810 535.7434 1069.4722 1069.4862 -13.1 0 3 0.55 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
3345 657.2986 1312.5826 1312.5870 -3.33 0 3 1.2 +1Score > 16 indicates identity SYITGEFGHMR + Oxidation (M)
4286 738.3355 1474.6564 1474.6663 -6.70 0 3 1.1 +1Score > 15 indicates identity SIGPWQHFQMAR + 2 Deamidated (NQ); Oxidation (M)
5310 819.3723 1636.7300 1636.7403 -6.24 0 3 1.3 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
1433 495.2250 988.4354 988.4461 -10.8 0 3 0.55 +1Score > 13 indicates identity EELDQEAR
5272 819.3723 1636.7300 1636.7119 11.1 1 3 1.4 +1Score > 16 indicates identity RMGELMAESHASMR + 2 Oxidation (M)
5299 819.3723 1636.7300 1636.7337 -2.26 0 3 1.4 +1Score > 16 indicates identity YMGNNGPMQLEVPR + 2 Oxidation (M)
2698 603.2741 1204.5336 1204.5394 -4.76 1 3 1.2 +1Score > 16 indicates identity ADEPKCGATEK
3716 684.3109 1366.6072 1366.6266 -14.1 1 3 1.2 +1Score > 16 indicates identity DWRETNIDYR
4269 738.3355 1474.6564 1474.6582 -1.22 1 3 1.1 +1Score > 15 indicates identity AECQNQQGNLRR + 2 Deamidated (NQ)
6418 913.9153 1825.8160 1825.8482 -17.6 1 3 1.6 +1Score > 17 indicates identity EDAQLLYGFNNKQER + 2 Deamidated (NQ)
3292 657.2986 1312.5826 1312.5830 -0.24 0 3 1.2 +1Score > 16 indicates identity NHCEILQGNAR + 2 Deamidated (NQ)
5294 819.3723 1636.7300 1636.7403 -6.24 0 3 1.4 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
6577 927.4214 1852.8282 1852.8546 -14.2 1 3 1.4 +1Score > 17 indicates identity MSKSIGNTVSPQDVMNK + 2 Deamidated (NQ); Oxidation (M)
6750 940.9276 1879.8406 1879.8258 7.90 0 3 1.7 +1Score > 17 indicates identity MSQLVYFSSSSENTQR + Deamidated (NQ); Oxidation (M)
1222 481.7188 961.4230 961.4175 5.82 0 2 0.9 +1Score > 15 indicates identity NLQPNMSR + 3 Deamidated (NQ)
6276 900.4092 1798.8038 1798.7870 9.35 0 2 0.98 +1Score > 15 indicates identity TQADAWDINQGHQSAR + 2 Deamidated (NQ)
2811 616.7802 1231.5458 1231.5615 -12.7 1 2 0.99 +1Score > 15 indicates identity IREMSDNDPR
3161 643.7925 1285.5704 1285.5761 -4.41 0 2 0.93 +1Score > 15 indicates identity MQAYFDQLDR
149 387.1811 772.3476 772.3537 -7.89 1 2 0.56 +1Score > 13 indicates identity HKDMNK + Deamidated (NQ)
2506 589.7679 1177.5212 1177.5363 -12.8 1 2 0.82 +1Score > 14 indicates identity EGENPYANKR + Deamidated (NQ)
3638 684.3109 1366.6072 1366.6252 -13.1 0 2 1.2 +1Score > 16 indicates identity YQVDINNLTQR + 4 Deamidated (NQ)
4458 751.8416 1501.6686 1501.6871 -12.3 1 2 1.5 +1Score > 17 indicates identity MNKTEFYADLNR + Deamidated (NQ)
2634 603.2741 1204.5336 1204.5394 -4.75 0 2 1.2 +1Score > 16 indicates identity ADCEQLAELR + Deamidated (NQ)
2669 603.2741 1204.5336 1204.5506 -14.1 0 2 1.2 +1Score > 16 indicates identity GCGITVDQAER
3048 630.2864 1258.5582 1258.5830 -19.6 1 2 0.76 +1Score > 14 indicates identity QVRSFTYDDK + Deamidated (NQ)
3553 670.8048 1339.5950 1339.6125 -13.0 1 2 1.4 +1Score > 16 indicates identity AANQAFMMERR + Oxidation (M)
5796 859.8907 1717.7668 1717.7875 -12.0 1 2 1 +1Score > 15 indicates identity AEDMAAANAMASHLRK + 2 Oxidation (M)
110 387.1803 772.3460 772.3351 14.1 0 2 0.57 +1Score > 13 indicates identity DPNGDVR + Deamidated (NQ)
1022 468.2127 934.4108 934.4178 -7.46 1 2 1.1 +1Score > 15 indicates identity LDEDCKR
1811 535.7434 1069.4722 1069.4862 -13.1 0 2 0.57 +1Score > 13 indicates identity YCVNSASLR + Deamidated (NQ)
6870 954.4337 1906.8528 1906.8690 -8.48 1 2 1.6 +1Score > 17 indicates identity LSAEDNARVNVAMNQTR + 3 Deamidated (NQ); Oxidation (M)
2255 562.7557 1123.4968 1123.5114 -13.0 0 2 0.88 +1Score > 14 indicates identity ISGTGMCNIR + Oxidation (M)
6521 927.4214 1852.8282 1852.8241 2.25 1 2 1.5 +1Score > 17 indicates identity EWYEKHGNSHQGEPR
705 441.1952 880.3758 880.3596 18.5 0 2 1 +1Score > 15 indicates identity LNSQMNR + 3 Deamidated (NQ); Oxidation (M)
2958 630.2834 1258.5522 1258.5621 -7.79 0 2 0.57 +1Score > 13 indicates identity ISMMPGMPSHR + Oxidation (M)
2041 549.2496 1096.4846 1096.4978 -12.0 0 2 0.97 +1Score > 15 indicates identity YYGYTGAFR
4535 751.8458 1501.6770 1501.6909 -9.25 1 2 1.7 +1Score > 17 indicates identity QNRSGAEHFGLQR + 3 Deamidated (NQ)
5597 846.3846 1690.7546 1690.7733 -11.0 0 2 1.2 +1Score > 16 indicates identity MAQEYTVEQLNHGR + Oxidation (M)
3640 684.3109 1366.6072 1366.6299 -16.6 1 2 1.3 +1Score > 16 indicates identity SRDAFIDEMQR
4206 724.8293 1447.6440 1447.6725 -19.7 1 2 0.73 +1Score > 13 indicates identity EVGEARQVDMAAR + Deamidated (NQ); Oxidation (M)
6569 927.4214 1852.8282 1852.8241 2.25 1 2 1.5 +1Score > 17 indicates identity EWYEKHGNSHQGEPR
3991 711.3232 1420.6318 1420.6187 9.25 1 2 1.4 +1Score > 16 indicates identity MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M)
5962 873.3969 1744.7792 1744.7735 3.28 0 2 2 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
3363 657.2986 1312.5826 1312.5870 -3.32 0 2 1.3 +1Score > 16 indicates identity FYEAVMSSHAR + Oxidation (M)
3679 684.3109 1366.6072 1366.6266 -14.1 1 2 1.3 +1Score > 16 indicates identity DWRETNIDYR
3984 711.3232 1420.6318 1420.6187 9.25 1 2 1.4 +1Score > 16 indicates identity MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M)
6728 940.9276 1879.8406 1879.8729 -17.2 0 2 1.8 +1Score > 17 indicates identity DLALNQAMIPLGSCTMK + 2 Deamidated (NQ); Oxidation (M)
2492 589.7679 1177.5212 1177.5363 -12.8 1 2 0.86 +1Score > 14 indicates identity EGENPYANKR + Deamidated (NQ)
3973 711.3232 1420.6318 1420.6187 9.25 1 2 1.5 +1Score > 16 indicates identity MRAQNCVGPNTR + 2 Deamidated (NQ); Oxidation (M)
657 441.1929 880.3712 880.3562 17.0 0 2 0.71 +1Score > 13 indicates identity EDEAFNR + Deamidated (NQ)
5918 873.3969 1744.7792 1744.7735 3.28 0 2 2.1 +1Score > 18 indicates identity GTLFPMCGMNLAFDR + Oxidation (M)
5587 846.3846 1690.7546 1690.7403 8.49 0 2 1.3 +1Score > 16 indicates identity SEMGDLAQSVSHMQR + Oxidation (M)
4309 738.3355 1474.6564 1474.6582 -1.22 1 2 1.2 +1Score > 15 indicates identity AECQNQQGNLRR + 2 Deamidated (NQ)
5296 819.3723 1636.7300 1636.7403 -6.24 0 2 1.5 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
1279 481.7188 961.4230 961.4352 -12.7 0 2 0.97 +1Score > 15 indicates identity VNDQEISR + 2 Deamidated (NQ)
2423 576.2618 1150.5090 1150.5077 1.19 1 2 1.1 +1Score > 15 indicates identity DTANMQRWK + 2 Deamidated (NQ)
5728 859.8907 1717.7668 1717.7907 -13.9 1 2 1.1 +1Score > 15 indicates identity SVVNNDPQGYPNRVR + 4 Deamidated (NQ)
6142 886.9030 1771.7914 1771.7968 -3.04 0 2 1.4 +1Score > 16 indicates identity SQSTVGAGDSMVGAMTLK + Deamidated (NQ); 2 Oxidation (M)
2172 562.7557 1123.4968 1123.5067 -8.75 0 2 0.94 +1Score > 14 indicates identity QQETAVATMK + 2 Deamidated (NQ); Oxidation (M)
7109 967.9399 1933.8652 1933.9017 -18.9 1 2 1.6 +1Score > 17 indicates identity QDIDRWQSTSVNDVLR + 3 Deamidated (NQ)
1624 522.2295 1042.4444 1042.4277 16.0 0 2 0.64 +1Score > 13 indicates identity FSLGNMDDK + Deamidated (NQ); Oxidation (M)
2365 576.2618 1150.5090 1150.5117 -2.32 0 2 1.1 +1Score > 15 indicates identity YMTWDNPPK
2457 589.7679 1177.5212 1177.5332 -10.1 1 2 0.88 +1Score > 14 indicates identity ANNPTSRMMR + Deamidated (NQ)
5328 819.3723 1636.7300 1636.7403 -6.24 0 2 1.5 +1Score > 16 indicates identity GFNQSDLLCQPLSR + 3 Deamidated (NQ)
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