MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3401 657.3086 1312.6026
3402 657.3099 1312.6052
3403 670.7943 1339.5740
3404 670.8048 1339.5950
3405 670.8048 1339.5950
3406 670.8048 1339.5950
3407 670.8048 1339.5950
3408 670.8048 1339.5950
3409 670.8048 1339.5950
3410 670.8048 1339.5950
3411 670.8048 1339.5950
3412 670.8048 1339.5950
3413 670.8048 1339.5950
3414 670.8048 1339.5950
3415 670.8048 1339.5950
3416 670.8048 1339.5950
3417 670.8048 1339.5950
3418 670.8048 1339.5950
3419 670.8048 1339.5950
3420 670.8048 1339.5950
3421 670.8048 1339.5950
3422 670.8048 1339.5950
3423 670.8048 1339.5950
3424 670.8048 1339.5950
3425 670.8048 1339.5950
3426 670.8048 1339.5950
3427 670.8048 1339.5950
3428 670.8048 1339.5950
3429 670.8048 1339.5950
3430 670.8048 1339.5950
3431 670.8048 1339.5950
3432 670.8048 1339.5950
3433 670.8048 1339.5950
3434 670.8048 1339.5950
3435 670.8048 1339.5950
3436 670.8048 1339.5950
3437 670.8048 1339.5950
3438 670.8048 1339.5950
3439 670.8048 1339.5950
3440 670.8048 1339.5950
3441 670.8048 1339.5950
3442 670.8048 1339.5950
3443 670.8048 1339.5950
3444 670.8048 1339.5950
3445 670.8048 1339.5950
3446 670.8048 1339.5950
3447 670.8048 1339.5950
3448 670.8048 1339.5950
3449 670.8048 1339.5950
3450 670.8048 1339.5950
3451 670.8048 1339.5950
3452 670.8048 1339.5950
3453 670.8048 1339.5950
3454 670.8048 1339.5950
3455 670.8048 1339.5950
3456 670.8048 1339.5950
3457 670.8048 1339.5950
3458 670.8048 1339.5950
3459 670.8048 1339.5950
3460 670.8048 1339.5950
3462 670.8048 1339.5950
3463 670.8048 1339.5950
3464 670.8048 1339.5950
3465 670.8048 1339.5950
3466 670.8048 1339.5950
3467 670.8048 1339.5950
3468 670.8048 1339.5950
3469 670.8048 1339.5950
3471 670.8048 1339.5950
3472 670.8048 1339.5950
3473 670.8048 1339.5950
3475 670.8048 1339.5950
3476 670.8048 1339.5950
3477 670.8048 1339.5950
3478 670.8048 1339.5950
3479 670.8048 1339.5950
3480 670.8048 1339.5950
3482 670.8048 1339.5950
3483 670.8048 1339.5950
3484 670.8048 1339.5950
3485 670.8048 1339.5950
3486 670.8048 1339.5950
3487 670.8048 1339.5950
3488 670.8048 1339.5950
3490 670.8048 1339.5950
3491 670.8048 1339.5950
3493 670.8048 1339.5950
3495 670.8048 1339.5950
3496 670.8048 1339.5950
3497 670.8048 1339.5950
3498 670.8048 1339.5950
3499 670.8048 1339.5950
3500 670.8048 1339.5950
3501 670.8048 1339.5950
3502 670.8048 1339.5950
3503 670.8048 1339.5950
3504 670.8048 1339.5950
3506 670.8048 1339.5950
3507 670.8048 1339.5950
3508 670.8048 1339.5950
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