MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3272 657.2986 1312.5826
3273 657.2986 1312.5826
3274 657.2986 1312.5826
3275 657.2986 1312.5826
3276 657.2986 1312.5826
3277 657.2986 1312.5826
3278 657.2986 1312.5826
3279 657.2986 1312.5826
3280 657.2986 1312.5826
3281 657.2986 1312.5826
3282 657.2986 1312.5826
3283 657.2986 1312.5826
3284 657.2986 1312.5826
3285 657.2986 1312.5826
3286 657.2986 1312.5826
3287 657.2986 1312.5826
3288 657.2986 1312.5826
3289 657.2986 1312.5826
3290 657.2986 1312.5826
3291 657.2986 1312.5826
3293 657.2986 1312.5826
3294 657.2986 1312.5826
3295 657.2986 1312.5826
3296 657.2986 1312.5826
3297 657.2986 1312.5826
3298 657.2986 1312.5826
3299 657.2986 1312.5826
3300 657.2986 1312.5826
3301 657.2986 1312.5826
3302 657.2986 1312.5826
3305 657.2986 1312.5826
3307 657.2986 1312.5826
3308 657.2986 1312.5826
3309 657.2986 1312.5826
3313 657.2986 1312.5826
3315 657.2986 1312.5826
3316 657.2986 1312.5826
3317 657.2986 1312.5826
3320 657.2986 1312.5826
3321 657.2986 1312.5826
3322 657.2986 1312.5826
3323 657.2986 1312.5826
3326 657.2986 1312.5826
3328 657.2986 1312.5826
3329 657.2986 1312.5826
3330 657.2986 1312.5826
3331 657.2986 1312.5826
3333 657.2986 1312.5826
3334 657.2986 1312.5826
3335 657.2986 1312.5826
3336 657.2986 1312.5826
3337 657.2986 1312.5826
3338 657.2986 1312.5826
3340 657.2986 1312.5826
3342 657.2986 1312.5826
3347 657.2986 1312.5826
3348 657.2986 1312.5826
3349 657.2986 1312.5826
3350 657.2986 1312.5826
3352 657.2986 1312.5826
3353 657.2986 1312.5826
3354 657.2986 1312.5826
3355 657.2986 1312.5826
3356 657.2986 1312.5826
3358 657.2986 1312.5826
3360 657.2986 1312.5826
3361 657.2986 1312.5826
3362 657.2986 1312.5826
3364 657.2986 1312.5826
3365 657.2986 1312.5826
3366 657.2986 1312.5826
3367 657.2986 1312.5826
3369 657.2986 1312.5826
3370 657.2986 1312.5826
3371 657.2986 1312.5826
3372 657.2986 1312.5826
3373 657.2986 1312.5826
3374 657.2986 1312.5826
3375 657.2986 1312.5826
3376 657.2986 1312.5826
3377 657.2986 1312.5826
3378 657.2986 1312.5826
3379 657.2986 1312.5826
3380 657.2986 1312.5826
3381 657.2986 1312.5826
3382 657.2986 1312.5826
3383 657.2986 1312.5826
3384 657.2986 1312.5826
3386 657.2986 1312.5826
3387 657.2986 1312.5826
3388 657.2986 1312.5826
3389 657.2986 1312.5826
3390 657.2986 1312.5826
3392 657.2986 1312.5826
3393 657.2986 1312.5826
3394 657.2986 1312.5826
3395 657.2986 1312.5826
3396 657.2995 1312.5844
3397 657.3015 1312.5884
3400 657.3055 1312.5964
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