MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3163 643.7925 1285.5704
3164 643.7925 1285.5704
3165 643.7925 1285.5704
3166 643.7925 1285.5704
3167 643.7925 1285.5704
3168 643.7925 1285.5704
3169 643.7925 1285.5704
3170 643.7925 1285.5704
3171 643.7925 1285.5704
3172 643.7925 1285.5704
3174 643.7925 1285.5704
3176 643.7925 1285.5704
3177 643.7925 1285.5704
3178 643.7925 1285.5704
3179 643.7925 1285.5704
3181 643.7925 1285.5704
3182 643.7925 1285.5704
3183 643.7925 1285.5704
3184 643.7925 1285.5704
3185 643.7925 1285.5704
3186 643.7925 1285.5704
3187 643.7925 1285.5704
3188 643.7925 1285.5704
3189 643.7925 1285.5704
3190 643.7925 1285.5704
3191 643.7925 1285.5704
3192 643.7925 1285.5704
3193 643.7925 1285.5704
3194 643.7925 1285.5704
3195 643.7925 1285.5704
3196 643.7925 1285.5704
3197 643.7925 1285.5704
3198 643.7925 1285.5704
3199 643.7925 1285.5704
3200 643.7925 1285.5704
3203 643.7925 1285.5704
3204 643.7925 1285.5704
3205 643.7925 1285.5704
3206 643.7925 1285.5704
3207 643.7925 1285.5704
3208 643.7925 1285.5704
3209 643.7925 1285.5704
3210 643.7925 1285.5704
3211 643.7925 1285.5704
3212 643.7925 1285.5704
3213 643.7925 1285.5704
3214 643.7925 1285.5704
3215 643.7925 1285.5704
3217 643.7925 1285.5704
3218 643.7925 1285.5704
3219 643.7925 1285.5704
3220 643.7925 1285.5704
3221 643.7925 1285.5704
3222 643.7925 1285.5704
3223 643.7925 1285.5704
3224 643.7925 1285.5704
3225 643.7925 1285.5704
3226 643.7925 1285.5704
3227 643.7925 1285.5704
3228 643.7925 1285.5704
3229 643.7925 1285.5704
3230 643.7925 1285.5704
3231 643.7925 1285.5704
3232 643.7925 1285.5704
3233 643.7925 1285.5704
3234 643.7925 1285.5704
3235 643.7986 1285.5826
3236 643.7993 1285.5840
3237 643.8005 1285.5864
3238 643.8012 1285.5878
3239 643.8025 1285.5904
3240 643.8037 1285.5928
3241 657.2859 1312.5572
3243 657.2886 1312.5626
3244 657.2891 1312.5636
3245 657.2904 1312.5662
3247 657.2911 1312.5676
3248 657.2913 1312.5680
3249 657.2916 1312.5686
3250 657.2917 1312.5688
3251 657.2926 1312.5706
3252 657.2933 1312.5720
3253 657.2943 1312.5740
3254 657.2950 1312.5754
3255 657.2950 1312.5754
3256 657.2962 1312.5778
3257 657.2965 1312.5784
3258 657.2965 1312.5784
3259 657.2968 1312.5790
3260 657.2969 1312.5792
3262 657.2972 1312.5798
3263 657.2982 1312.5818
3264 657.2985 1312.5824
3265 657.2985 1312.5824
3266 657.2986 1312.5826
3267 657.2986 1312.5826
3268 657.2986 1312.5826
3269 657.2986 1312.5826
3270 657.2986 1312.5826
3271 657.2986 1312.5826
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