MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2919 630.2784 1258.5422
2920 630.2787 1258.5428
2921 630.2788 1258.5430
2922 630.2792 1258.5438
2923 630.2795 1258.5444
2924 630.2802 1258.5458
2925 630.2802 1258.5458
2926 630.2806 1258.5466
2928 630.2809 1258.5472
2929 630.2809 1258.5472
2932 630.2816 1258.5486
2934 630.2819 1258.5492
2935 630.2820 1258.5494
2936 630.2821 1258.5496
2937 630.2821 1258.5496
2938 630.2825 1258.5504
2939 630.2825 1258.5504
2940 630.2827 1258.5508
2941 630.2827 1258.5508
2942 630.2827 1258.5508
2943 630.2828 1258.5510
2944 630.2828 1258.5510
2945 630.2829 1258.5512
2948 630.2831 1258.5516
2949 630.2831 1258.5516
2950 630.2831 1258.5516
2951 630.2832 1258.5518
2952 630.2833 1258.5520
2953 630.2833 1258.5520
2954 630.2833 1258.5520
2956 630.2834 1258.5522
2957 630.2834 1258.5522
2959 630.2834 1258.5522
2960 630.2835 1258.5524
2961 630.2836 1258.5526
2963 630.2836 1258.5526
2964 630.2836 1258.5526
2965 630.2836 1258.5526
2966 630.2838 1258.5530
2967 630.2839 1258.5532
2968 630.2840 1258.5534
2970 630.2841 1258.5536
2973 630.2842 1258.5538
2974 630.2843 1258.5540
2975 630.2843 1258.5540
2977 630.2844 1258.5542
2979 630.2844 1258.5542
2980 630.2844 1258.5542
2981 630.2844 1258.5542
2982 630.2844 1258.5542
2983 630.2844 1258.5542
2984 630.2845 1258.5544
2986 630.2846 1258.5546
2987 630.2846 1258.5546
2988 630.2847 1258.5548
2990 630.2849 1258.5552
2991 630.2850 1258.5554
2993 630.2852 1258.5558
2994 630.2852 1258.5558
2995 630.2853 1258.5560
2997 630.2858 1258.5570
2998 630.2861 1258.5576
2999 630.2864 1258.5582
3000 630.2864 1258.5582
3001 630.2864 1258.5582
3002 630.2864 1258.5582
3003 630.2864 1258.5582
3004 630.2864 1258.5582
3005 630.2864 1258.5582
3006 630.2864 1258.5582
3007 630.2864 1258.5582
3008 630.2864 1258.5582
3009 630.2864 1258.5582
3010 630.2864 1258.5582
3011 630.2864 1258.5582
3012 630.2864 1258.5582
3013 630.2864 1258.5582
3014 630.2864 1258.5582
3015 630.2864 1258.5582
3016 630.2864 1258.5582
3018 630.2864 1258.5582
3019 630.2864 1258.5582
3020 630.2864 1258.5582
3021 630.2864 1258.5582
3022 630.2864 1258.5582
3023 630.2864 1258.5582
3024 630.2864 1258.5582
3025 630.2864 1258.5582
3026 630.2864 1258.5582
3028 630.2864 1258.5582
3029 630.2864 1258.5582
3030 630.2864 1258.5582
3031 630.2864 1258.5582
3032 630.2864 1258.5582
3033 630.2864 1258.5582
3034 630.2864 1258.5582
3035 630.2864 1258.5582
3036 630.2864 1258.5582
3037 630.2864 1258.5582
3038 630.2864 1258.5582
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