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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2800 616.7802 1231.5458
2801 616.7802 1231.5458
2802 616.7802 1231.5458
2804 616.7802 1231.5458
2805 616.7802 1231.5458
2806 616.7802 1231.5458
2807 616.7802 1231.5458
2809 616.7802 1231.5458
2810 616.7802 1231.5458
2812 616.7802 1231.5458
2813 616.7802 1231.5458
2814 616.7802 1231.5458
2815 616.7802 1231.5458
2816 616.7802 1231.5458
2817 616.7802 1231.5458
2818 616.7802 1231.5458
2819 616.7802 1231.5458
2820 616.7802 1231.5458
2821 616.7802 1231.5458
2822 616.7802 1231.5458
2824 616.7802 1231.5458
2825 616.7802 1231.5458
2826 616.7802 1231.5458
2827 616.7802 1231.5458
2829 616.7802 1231.5458
2830 616.7802 1231.5458
2831 616.7802 1231.5458
2832 616.7802 1231.5458
2833 616.7802 1231.5458
2835 616.7802 1231.5458
2836 616.7802 1231.5458
2838 616.7802 1231.5458
2839 616.7802 1231.5458
2840 616.7802 1231.5458
2841 616.7802 1231.5458
2842 616.7802 1231.5458
2843 616.7802 1231.5458
2844 616.7802 1231.5458
2846 616.7802 1231.5458
2847 616.7802 1231.5458
2848 616.7802 1231.5458
2849 616.7802 1231.5458
2852 616.7802 1231.5458
2853 616.7802 1231.5458
2854 616.7802 1231.5458
2855 616.7802 1231.5458
2856 616.7802 1231.5458
2857 616.7802 1231.5458
2858 616.7802 1231.5458
2860 616.7802 1231.5458
2861 616.7802 1231.5458
2862 616.7802 1231.5458
2863 616.7802 1231.5458
2864 616.7802 1231.5458
2866 616.7802 1231.5458
2867 616.7802 1231.5458
2868 616.7802 1231.5458
2869 616.7802 1231.5458
2870 616.7802 1231.5458
2872 616.7802 1231.5458
2873 616.7802 1231.5458
2874 616.7802 1231.5458
2875 616.7802 1231.5458
2877 616.7802 1231.5458
2878 616.7802 1231.5458
2879 616.7802 1231.5458
2881 616.7802 1231.5458
2882 616.7802 1231.5458
2883 616.7802 1231.5458
2884 616.7802 1231.5458
2885 616.7802 1231.5458
2886 616.7802 1231.5458
2887 616.7802 1231.5458
2888 616.7802 1231.5458
2889 616.7802 1231.5458
2890 616.7802 1231.5458
2891 616.7802 1231.5458
2893 616.7802 1231.5458
2895 616.7802 1231.5458
2896 616.7802 1231.5458
2897 616.7802 1231.5458
2898 616.7802 1231.5458
2899 616.7802 1231.5458
2900 616.7802 1231.5458
2901 616.7802 1231.5458
2902 616.7802 1231.5458
2903 616.7802 1231.5458
2906 616.7802 1231.5458
2907 616.7802 1231.5458
2908 616.7802 1231.5458
2909 616.7802 1231.5458
2910 616.7802 1231.5458
2911 616.7802 1231.5458
2912 616.7802 1231.5458
2913 616.7802 1231.5458
2914 616.7802 1231.5458
2915 616.7802 1231.5458
2916 616.7921 1231.5696
2917 630.2772 1258.5398
2918 630.2783 1258.5420
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