MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2691 603.2741 1204.5336
2692 603.2741 1204.5336
2693 603.2741 1204.5336
2694 603.2741 1204.5336
2695 603.2741 1204.5336
2696 603.2741 1204.5336
2697 603.2741 1204.5336
2699 603.2741 1204.5336
2700 603.2741 1204.5336
2701 603.2741 1204.5336
2702 603.2741 1204.5336
2703 603.2741 1204.5336
2704 603.2741 1204.5336
2705 603.2741 1204.5336
2706 603.2741 1204.5336
2707 603.2741 1204.5336
2708 603.2741 1204.5336
2709 603.2741 1204.5336
2710 603.2741 1204.5336
2711 603.2741 1204.5336
2712 603.2741 1204.5336
2713 603.2741 1204.5336
2714 603.2741 1204.5336
2715 603.2741 1204.5336
2716 603.2741 1204.5336
2717 603.2741 1204.5336
2718 603.2741 1204.5336
2719 603.2741 1204.5336
2720 603.2741 1204.5336
2721 603.2741 1204.5336
2722 603.2741 1204.5336
2723 603.2741 1204.5336
2724 603.2741 1204.5336
2725 603.2741 1204.5336
2726 603.2741 1204.5336
2727 603.2741 1204.5336
2728 603.2741 1204.5336
2729 603.2741 1204.5336
2730 603.2741 1204.5336
2731 603.2741 1204.5336
2732 603.2741 1204.5336
2735 603.2741 1204.5336
2736 603.2741 1204.5336
2737 603.2741 1204.5336
2738 603.2741 1204.5336
2739 603.2741 1204.5336
2740 603.2741 1204.5336
2741 603.2741 1204.5336
2742 603.2741 1204.5336
2743 603.2741 1204.5336
2744 603.2741 1204.5336
2745 603.2741 1204.5336
2746 603.2741 1204.5336
2747 603.2741 1204.5336
2748 603.2741 1204.5336
2749 603.2741 1204.5336
2750 603.2741 1204.5336
2751 603.2787 1204.5428
2752 603.2795 1204.5444
2754 603.2818 1204.5490
2755 616.7802 1231.5458
2756 616.7802 1231.5458
2757 616.7802 1231.5458
2758 616.7802 1231.5458
2759 616.7802 1231.5458
2760 616.7802 1231.5458
2761 616.7802 1231.5458
2762 616.7802 1231.5458
2763 616.7802 1231.5458
2764 616.7802 1231.5458
2765 616.7802 1231.5458
2766 616.7802 1231.5458
2767 616.7802 1231.5458
2768 616.7802 1231.5458
2769 616.7802 1231.5458
2770 616.7802 1231.5458
2771 616.7802 1231.5458
2772 616.7802 1231.5458
2773 616.7802 1231.5458
2774 616.7802 1231.5458
2775 616.7802 1231.5458
2776 616.7802 1231.5458
2777 616.7802 1231.5458
2778 616.7802 1231.5458
2779 616.7802 1231.5458
2780 616.7802 1231.5458
2781 616.7802 1231.5458
2782 616.7802 1231.5458
2783 616.7802 1231.5458
2784 616.7802 1231.5458
2787 616.7802 1231.5458
2790 616.7802 1231.5458
2791 616.7802 1231.5458
2792 616.7802 1231.5458
2793 616.7802 1231.5458
2794 616.7802 1231.5458
2795 616.7802 1231.5458
2796 616.7802 1231.5458
2798 616.7802 1231.5458
2799 616.7802 1231.5458
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