MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2582 589.7679 1177.5212
2583 589.7679 1177.5212
2584 589.7679 1177.5212
2585 589.7679 1177.5212
2586 589.7679 1177.5212
2587 589.7679 1177.5212
2588 589.7679 1177.5212
2589 589.7679 1177.5212
2590 589.7679 1177.5212
2591 589.7737 1177.5328
2592 589.7789 1177.5432
2593 603.2741 1204.5336
2594 603.2741 1204.5336
2595 603.2741 1204.5336
2596 603.2741 1204.5336
2597 603.2741 1204.5336
2598 603.2741 1204.5336
2599 603.2741 1204.5336
2600 603.2741 1204.5336
2601 603.2741 1204.5336
2602 603.2741 1204.5336
2603 603.2741 1204.5336
2604 603.2741 1204.5336
2605 603.2741 1204.5336
2606 603.2741 1204.5336
2607 603.2741 1204.5336
2608 603.2741 1204.5336
2609 603.2741 1204.5336
2610 603.2741 1204.5336
2611 603.2741 1204.5336
2612 603.2741 1204.5336
2613 603.2741 1204.5336
2614 603.2741 1204.5336
2615 603.2741 1204.5336
2616 603.2741 1204.5336
2618 603.2741 1204.5336
2619 603.2741 1204.5336
2620 603.2741 1204.5336
2621 603.2741 1204.5336
2622 603.2741 1204.5336
2623 603.2741 1204.5336
2624 603.2741 1204.5336
2625 603.2741 1204.5336
2626 603.2741 1204.5336
2627 603.2741 1204.5336
2628 603.2741 1204.5336
2629 603.2741 1204.5336
2630 603.2741 1204.5336
2631 603.2741 1204.5336
2632 603.2741 1204.5336
2633 603.2741 1204.5336
2635 603.2741 1204.5336
2636 603.2741 1204.5336
2637 603.2741 1204.5336
2638 603.2741 1204.5336
2639 603.2741 1204.5336
2641 603.2741 1204.5336
2643 603.2741 1204.5336
2644 603.2741 1204.5336
2645 603.2741 1204.5336
2646 603.2741 1204.5336
2647 603.2741 1204.5336
2648 603.2741 1204.5336
2649 603.2741 1204.5336
2650 603.2741 1204.5336
2651 603.2741 1204.5336
2652 603.2741 1204.5336
2653 603.2741 1204.5336
2655 603.2741 1204.5336
2657 603.2741 1204.5336
2658 603.2741 1204.5336
2659 603.2741 1204.5336
2660 603.2741 1204.5336
2661 603.2741 1204.5336
2662 603.2741 1204.5336
2663 603.2741 1204.5336
2664 603.2741 1204.5336
2665 603.2741 1204.5336
2666 603.2741 1204.5336
2667 603.2741 1204.5336
2668 603.2741 1204.5336
2670 603.2741 1204.5336
2671 603.2741 1204.5336
2672 603.2741 1204.5336
2673 603.2741 1204.5336
2674 603.2741 1204.5336
2676 603.2741 1204.5336
2677 603.2741 1204.5336
2678 603.2741 1204.5336
2680 603.2741 1204.5336
2681 603.2741 1204.5336
2682 603.2741 1204.5336
2683 603.2741 1204.5336
2684 603.2741 1204.5336
2685 603.2741 1204.5336
2686 603.2741 1204.5336
2687 603.2741 1204.5336
2688 603.2741 1204.5336
2689 603.2741 1204.5336
2690 603.2741 1204.5336
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