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Search metadata
User : Jennifer
E-mail : [email protected]
Search title : PRT1377_JBEI_2
MS data file : PRT1377_JBEI_2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 23 Jan 2026 at 21:31:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 7,287

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 15 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 7204)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2464 589.7679 1177.5212
2465 589.7679 1177.5212
2466 589.7679 1177.5212
2468 589.7679 1177.5212
2469 589.7679 1177.5212
2471 589.7679 1177.5212
2473 589.7679 1177.5212
2474 589.7679 1177.5212
2476 589.7679 1177.5212
2477 589.7679 1177.5212
2478 589.7679 1177.5212
2479 589.7679 1177.5212
2480 589.7679 1177.5212
2481 589.7679 1177.5212
2482 589.7679 1177.5212
2483 589.7679 1177.5212
2484 589.7679 1177.5212
2485 589.7679 1177.5212
2486 589.7679 1177.5212
2489 589.7679 1177.5212
2490 589.7679 1177.5212
2491 589.7679 1177.5212
2493 589.7679 1177.5212
2494 589.7679 1177.5212
2495 589.7679 1177.5212
2496 589.7679 1177.5212
2497 589.7679 1177.5212
2498 589.7679 1177.5212
2499 589.7679 1177.5212
2500 589.7679 1177.5212
2501 589.7679 1177.5212
2503 589.7679 1177.5212
2504 589.7679 1177.5212
2507 589.7679 1177.5212
2508 589.7679 1177.5212
2509 589.7679 1177.5212
2511 589.7679 1177.5212
2512 589.7679 1177.5212
2513 589.7679 1177.5212
2515 589.7679 1177.5212
2516 589.7679 1177.5212
2517 589.7679 1177.5212
2518 589.7679 1177.5212
2519 589.7679 1177.5212
2520 589.7679 1177.5212
2522 589.7679 1177.5212
2523 589.7679 1177.5212
2524 589.7679 1177.5212
2525 589.7679 1177.5212
2526 589.7679 1177.5212
2528 589.7679 1177.5212
2529 589.7679 1177.5212
2531 589.7679 1177.5212
2532 589.7679 1177.5212
2533 589.7679 1177.5212
2534 589.7679 1177.5212
2535 589.7679 1177.5212
2536 589.7679 1177.5212
2538 589.7679 1177.5212
2539 589.7679 1177.5212
2540 589.7679 1177.5212
2541 589.7679 1177.5212
2542 589.7679 1177.5212
2543 589.7679 1177.5212
2544 589.7679 1177.5212
2545 589.7679 1177.5212
2546 589.7679 1177.5212
2547 589.7679 1177.5212
2548 589.7679 1177.5212
2549 589.7679 1177.5212
2551 589.7679 1177.5212
2552 589.7679 1177.5212
2553 589.7679 1177.5212
2554 589.7679 1177.5212
2555 589.7679 1177.5212
2556 589.7679 1177.5212
2557 589.7679 1177.5212
2558 589.7679 1177.5212
2559 589.7679 1177.5212
2560 589.7679 1177.5212
2561 589.7679 1177.5212
2562 589.7679 1177.5212
2563 589.7679 1177.5212
2564 589.7679 1177.5212
2565 589.7679 1177.5212
2566 589.7679 1177.5212
2567 589.7679 1177.5212
2568 589.7679 1177.5212
2569 589.7679 1177.5212
2571 589.7679 1177.5212
2572 589.7679 1177.5212
2573 589.7679 1177.5212
2574 589.7679 1177.5212
2575 589.7679 1177.5212
2576 589.7679 1177.5212
2577 589.7679 1177.5212
2578 589.7679 1177.5212
2579 589.7679 1177.5212
2580 589.7679 1177.5212
2581 589.7679 1177.5212
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